FastQCFastQC Report
Thu 26 May 2016
SRR1511865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969176
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67890.34476349498470427No Hit
GTCCTACAGTGGACATTTCTAAATT59620.3027662331858605No Hit
CTGTAGGACGTGGAATATGGCAAGA57930.29418396324147766No Hit
GTCCTAAAGTGTGTATTTCTCATTT55470.2816914282928494No Hit
CTTTAGGACGTGAAATATGGCGAGG50040.2541164426135602No Hit
GGTATCAACGCAGAGTACTTTTTTT45800.2325845937590139No Hit
TATCAACGCAGAGTACTTTTTTTTT43180.2192795362121009No Hit
CTGAAGGACCTGGAATATGGCGAGA27140.13782414573405322No Hit
GTCCTACAGTGTGCATTTCTCATTT26880.13650379651184047No Hit
CTGTAGGACCTGGAATATGGCGAGA22150.11248359720004712No Hit
ATTTAGAAATGTCCACTGTAGGACG21540.10938585479408647No Hit
GTCCTTCAGTGTGCATTTCTCATTT20940.1063388950505186No Hit
TTTCTAAATTTTCCACCTTTTTCAG20670.10496776316591305No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20620.10471384985394908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.013.49991
TCCAACG1405.820766E-1112.1452818
ACCTTTT8400.011.3575115
GTGGTAT3750.011.1845761
GCCCTAA905.7314337E-511.0958091
GGCGAGG14550.011.03694819
ATTCCGT600.00613196711.0201586
GTATCAA23050.010.9176991
TAGGACC9000.010.7055684
GTCCTAC30200.010.6475681
CCACCTT8350.010.63365213
AATGTCC8500.010.5565898
AATCCCG2250.010.49592119
CACCTTT8650.010.48325514
GTCCTAA16300.010.476351
TGGTATC3450.010.4204122
CGGTAGG1353.2502066E-710.3560881
TCCTACA33250.010.2415162
CCTACAC2900.010.0975453
CCTACAG33850.010.0181573
Produced by FastQC (version 0.11.5)