FastQCFastQC Report
Thu 26 May 2016
SRR1511865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969176
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86490.4392192470353082No Hit
GGTATCAACGCAGAGTACTTTTTTT59490.3021060585747541No Hit
CTGTAGGACGTGGAATATGGCAAGA59480.3020552759123613No Hit
TATCAACGCAGAGTACTTTTTTTTT59370.3014966666260405No Hit
GTCCTACAGTGGACATTTCTAAATT58780.29850048954486547No Hit
GTCCTAAAGTGTGTATTTCTCATTT55710.2829102121902765No Hit
CTTTAGGACGTGAAATATGGCGAGG55390.28128516699370704No Hit
CTGAAGGACCTGGAATATGGCGAGA30350.15412538036214132No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28700.1457462410673297No Hit
GTCCTACAGTGTGCATTTCTCATTT26240.1332537061187014No Hit
TTTCTAAATTTTCCACCTTTTTCAG24770.12578865474696016No Hit
CTGTAGGACCTGGAATATGGCGAGA23130.1174602981145413No Hit
ATTTAGAAATGTCCACTGTAGGACG22910.11634307954189976No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21810.11075698667869201No Hit
GAATATGGCAAGAAAACTGAAAATC21010.10669437368726817No Hit
GTCCTTCAGTGTGCATTTCTCATTT20090.10202236874713078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.014.3836491
TCCAACG1700.012.85022718
TAGGACC9100.012.2138784
TTATCCG550.003069714612.0905055
CCAACGA1201.0053554E-811.8721919
AACCGTT658.044462E-411.6889497
ATTGCGA752.079404E-411.39701417
TTGCGAG855.3357675E-511.1741118
CGGTTTC803.780779E-410.684713
ACGAAAT2250.010.55279112
TCTACAC2900.010.4824093
GACGTGG17350.010.4552097
CGAAATC2400.010.28897113
CCACCTT8050.010.26439113
AGGACCT18700.010.2616885
GATATAC6000.010.1727841
GGCGAGG14300.010.16193119
ATTACCC750.002648841610.1329953
AATGCCG2250.010.13119318
TGTAGGA33900.010.0321132
Produced by FastQC (version 0.11.5)