##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511865_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1969176 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.425766919767455 31.0 31.0 34.0 27.0 34.0 2 30.635678070421335 31.0 31.0 34.0 27.0 34.0 3 30.734290891215412 31.0 31.0 34.0 27.0 34.0 4 34.32833733500713 37.0 35.0 37.0 30.0 37.0 5 33.907696417181604 37.0 35.0 37.0 30.0 37.0 6 33.8456080106603 37.0 35.0 37.0 29.0 37.0 7 33.668432379838066 37.0 35.0 37.0 28.0 37.0 8 33.559864633735124 37.0 35.0 37.0 28.0 37.0 9 34.999032590281416 38.0 35.0 39.0 28.0 39.0 10 34.763762609335075 38.0 35.0 39.0 28.0 39.0 11 34.74255526169322 38.0 35.0 39.0 27.0 39.0 12 34.56411412692415 38.0 35.0 39.0 27.0 39.0 13 34.5490062848623 38.0 35.0 39.0 27.0 39.0 14 35.46089531865105 39.0 35.0 41.0 27.0 41.0 15 35.39959962948969 39.0 35.0 41.0 27.0 41.0 16 35.28881623582656 39.0 35.0 40.0 27.0 41.0 17 35.23485711790109 39.0 35.0 40.0 27.0 41.0 18 35.15451437555607 39.0 35.0 40.0 26.0 41.0 19 35.177669746127314 39.0 35.0 40.0 26.0 41.0 20 35.040592105530436 39.0 34.0 40.0 25.0 41.0 21 34.90294925390113 39.0 34.0 40.0 25.0 41.0 22 34.99584750169614 39.0 35.0 40.0 25.0 41.0 23 34.870499132632126 39.0 35.0 40.0 25.0 41.0 24 34.732547522415466 39.0 34.0 41.0 24.0 41.0 25 34.273154354918 38.0 34.0 40.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34309389582248073 1101 2 0.24077989875397066 1101 3 0.3793169526179554 1101 4 0.320755390751323 1101 5 0.38102084813021975 1101 6 0.31213447623848367 1101 7 0.382650650005651 1101 8 0.41848073590418267 1101 9 0.43871222856316905 1101 10 0.41154022550990277 1101 11 0.05403059596365978 1101 12 0.30578655606598204 1101 13 0.36654228751947926 1101 14 0.41483604393861384 1101 15 0.3088270150211798 1101 16 0.2319026339399386 1101 17 0.17083620882930717 1101 18 0.3849225568136774 1101 19 0.12009525595276216 1101 20 0.3050082846711888 1101 21 0.2602119932920104 1101 22 0.22831782017195223 1101 23 0.02762498304349492 1101 24 -0.13250334178369627 1101 25 -0.021297647372591655 1102 1 0.5560358464629971 1102 2 0.39022978590242374 1102 3 0.6738256640193434 1102 4 0.5262866540121465 1102 5 0.5436070242770299 1102 6 0.5515089035245708 1102 7 0.6290421679973264 1102 8 0.6556104321582481 1102 9 0.7505084438851455 1102 10 0.8422579859448334 1102 11 0.8432165273425412 1102 12 0.9152833058184413 1102 13 1.0260565646238362 1102 14 1.2625018311627088 1102 15 1.3773743357963184 1102 16 1.1679739241375344 1102 17 1.2439221912527572 1102 18 1.1452037324377429 1102 19 1.139086295478478 1102 20 1.1598818109898943 1102 21 1.250212177790452 1102 22 1.0937446573151774 1102 23 1.4425927265660619 1102 24 1.339874166626359 1102 25 1.5773997936340294 1103 1 0.48957346988826345 1103 2 0.4823821279424081 1103 3 0.4993269043520421 1103 4 0.671918250879699 1103 5 0.7464087169663642 1103 6 0.6223917285647005 1103 7 0.642311295873192 1103 8 0.558804167262096 1103 9 0.7305269272744184 1103 10 0.8764892228726993 1103 11 0.9435404730597412 1103 12 0.7776559893147237 1103 13 0.695793419529231 1103 14 0.991807731255129 1103 15 1.0442044345365247 1103 16 0.8276811083390996 1103 17 0.8465390500493868 1103 18 0.9363959110456506 1103 19 0.9430320126826217 1103 20 0.9661875651608156 1103 21 0.8221466103990451 1103 22 1.0595523327857705 1103 23 1.0769796130877793 1103 24 1.1203304144983335 1103 25 1.0803055769892538 1104 1 0.5266778817219162 1104 2 0.5200604063427079 1104 3 0.5753792274801803 1104 4 0.4670685157772567 1104 5 0.6187702904109713 1104 6 0.5817109652846568 1104 7 0.7553592587406541 1104 8 0.6948367988903996 1104 9 0.8203482972401943 1104 10 0.7240882664284172 1104 11 0.8238274063430708 1104 12 0.9181632798581134 1104 13 0.9685807044464809 1104 14 0.9072374279122641 1104 15 0.9039653234670908 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-0.8499623114715433 2313 25 -1.0297728899154777 2314 1 -0.5880405827257924 2314 2 -0.5624547018944241 2314 3 -0.6372981372729996 2314 4 -0.5894220754606252 2314 5 -0.6645719690143181 2314 6 -0.6739818978089289 2314 7 -0.8186777151158182 2314 8 -0.7884052899473559 2314 9 -0.7756419038944102 2314 10 -0.7086073472238752 2314 11 -0.8253639558446864 2314 12 -0.8165714499127148 2314 13 -0.7282958245600142 2314 14 -0.7843660949186955 2314 15 -0.7598648295327379 2314 16 -0.7860018753438851 2314 17 -0.6766544231440861 2314 18 -0.8581145784648356 2314 19 -0.8663053166547101 2314 20 -0.8101710761978254 2314 21 -0.6490655108130738 2314 22 -0.6278619196284794 2314 23 -0.5827756316674666 2314 24 -0.9473269281590078 2314 25 -0.9026131709294916 2315 1 -0.618886842362663 2315 2 -0.7203985931415637 2315 3 -0.7296749241443692 2315 4 -0.8967788282144937 2315 5 -0.9513689566235826 2315 6 -1.0427733049577341 2315 7 -0.8900765329045299 2315 8 -1.0027521644927901 2315 9 -1.077795075632423 2315 10 -1.0303599027309431 2315 11 -1.139684713306238 2315 12 -1.1530718929113561 2315 13 -1.1830438442899833 2315 14 -1.378734722680825 2315 15 -1.4327417420305864 2315 16 -1.2508396849470387 2315 17 -1.3773146233530227 2315 18 -1.3379416106384383 2315 19 -1.4500089175063238 2315 20 -1.300446594902951 2315 21 -1.3618470133057698 2315 22 -1.450234342632669 2315 23 -1.3311986887547818 2315 24 -1.3271073462968275 2315 25 -1.4241515002295557 2316 1 -1.64540219077249 2316 2 -1.3232954828815124 2316 3 -1.2510505212878051 2316 4 -1.1131799208046331 2316 5 -1.1145138254238987 2316 6 -1.170476694234054 2316 7 -1.2574431995153006 2316 8 -1.2834445825946403 2316 9 -1.532119576245286 2316 10 -1.4606232327163013 2316 11 -1.4400501676047455 2316 12 -1.4260083630537608 2316 13 -1.3735093353099828 2316 14 -1.6151146543017205 2316 15 -1.7503874668273198 2316 16 -1.6204117370412519 2316 17 -1.7044999713101134 2316 18 -1.752241990764567 2316 19 -1.4802727086063072 2316 20 -1.6721519309213662 2316 21 -2.0804513954037915 2316 22 -1.9060972893060182 2316 23 -1.6932367567012818 2316 24 -1.6761878837384927 2316 25 -1.7087906403094735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41384.0 3 2.0 4 779.0 5 6433.0 6 8052.0 7 6232.0 8 4985.0 9 4542.0 10 4251.0 11 4015.0 12 3705.0 13 3538.0 14 3511.0 15 3676.0 16 3667.0 17 3772.0 18 4183.0 19 4545.0 20 5766.0 21 7167.0 22 9204.0 23 11557.0 24 13719.0 25 16490.0 26 18750.0 27 21514.0 28 25390.0 29 30532.0 30 38463.0 31 49308.0 32 62987.0 33 82057.0 34 107025.0 35 144187.0 36 213225.0 37 376555.0 38 624008.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.5049901023816 21.273634610414142 14.166670490109265 24.054704797095 2 14.236767053833685 22.13382653455049 42.22329542915412 21.406110982461698 3 18.329087902757298 26.713965638419317 31.229559978386902 23.72738648043649 4 12.312662758432968 18.890083974210533 39.65826315169391 29.138990115662594 5 11.91885420190648 39.25865083452958 36.84019639814818 11.98229856541576 6 29.52371956595043 39.51221221465222 18.74322051457056 12.22084770482679 7 26.258597504743104 32.61115309144535 23.797771250512906 17.332478153298638 8 23.35733321958017 39.37550528749081 21.406009417136914 15.86115207579211 9 25.861984911455348 15.239470722779478 21.665204125989753 37.23334023977542 10 14.324316363798866 30.205578373898522 35.83742641592219 19.632678846380415 11 32.50770880815123 22.677759631439752 26.889064258349688 17.92546730205934 12 22.932587031326808 25.514733066013402 33.45866494411876 18.094014958541035 13 28.577283086935857 23.223673252162325 26.730774699671333 21.468268961230486 14 19.429700544796404 24.254612081398513 29.08104709787241 27.234640275932676 15 21.36457076462439 34.07668994543911 24.127756990741307 20.430982299195197 16 19.963152044030664 29.43575805680862 30.459538090355696 20.141551808805016 17 20.02517804401435 30.346652610025714 29.574502228343224 20.05366711761671 18 19.63563328712097 28.19822564836962 32.97785361345135 19.18828745105806 19 22.484531601035155 27.441021015897004 29.44226417547238 20.63218320759546 20 22.60950773318383 28.458451656936706 30.129404380309328 18.802636229570137 21 21.336183256346818 27.58651334365237 29.02183451352241 22.055468886478405 22 21.822833510057 29.88498742621279 29.20648027398262 19.08569878974759 23 20.217085725196732 29.550177333057075 30.123564374134155 20.10917256761204 24 21.98882923291628 27.81661171681222 30.87628258648254 19.318276463788962 25 21.12064303136393 28.827076138053894 30.322932520403235 19.729348310178942 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 239.0 2 551.0 3 863.0 4 863.0 5 863.0 6 2200.0 7 3537.0 8 3537.0 9 3537.0 10 5061.5 11 6586.0 12 6586.0 13 6586.0 14 9980.0 15 13374.0 16 13374.0 17 13374.0 18 23097.5 19 32821.0 20 32821.0 21 32821.0 22 51348.0 23 69875.0 24 69875.0 25 69875.0 26 99828.5 27 129782.0 28 129782.0 29 129782.0 30 164732.0 31 199682.0 32 199682.0 33 199682.0 34 217615.0 35 235548.0 36 235548.0 37 235548.0 38 241571.5 39 247595.0 40 247595.0 41 247595.0 42 251136.5 43 254678.0 44 254678.0 45 254678.0 46 249943.0 47 245208.0 48 245208.0 49 245208.0 50 225974.5 51 206741.0 52 206741.0 53 206741.0 54 173563.0 55 140385.0 56 140385.0 57 140385.0 58 114984.5 59 89584.0 60 89584.0 61 89584.0 62 70446.0 63 51308.0 64 51308.0 65 51308.0 66 38252.0 67 25196.0 68 25196.0 69 25196.0 70 17912.0 71 10628.0 72 10628.0 73 10628.0 74 7219.5 75 3811.0 76 3811.0 77 3811.0 78 2559.0 79 1307.0 80 1307.0 81 1307.0 82 814.5 83 322.0 84 322.0 85 322.0 86 202.0 87 82.0 88 82.0 89 82.0 90 52.0 91 22.0 92 22.0 93 22.0 94 12.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3855927555485137 2 0.0 3 0.0 4 0.0 5 0.026508549769040456 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.523479871783934E-4 17 0.0 18 3.046959743567868E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005484527538422162 25 1.523479871783934E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1969176.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.15478095837615 #Duplication Level Percentage of deduplicated Percentage of total 1 88.59876905658453 65.7002231257278 2 8.415241613825975 12.48060797170154 3 1.5887836560583755 3.534477120157706 4 0.5039249893390118 1.4947378881554583 5 0.22753623840469678 0.8436449959496573 6 0.12887886528052428 0.5734190415024808 7 0.09475503565683674 0.4918577239685095 8 0.06522589931564503 0.3869449821251803 9 0.05019892770187831 0.3350241439270332 >10 0.2787733462500798 3.7925760980014753 >50 0.022587146214928282 1.1642065723093322 >100 0.020207154815572826 3.1083032588636326 >500 0.0023884329243296226 1.2115590549482609 >1k 0.0022519510429393477 2.682615012150904 >5k 4.7768658486592225E-4 2.199803010511053 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8649 0.4392192470353082 No Hit GGTATCAACGCAGAGTACTTTTTTT 5949 0.3021060585747541 No Hit CTGTAGGACGTGGAATATGGCAAGA 5948 0.3020552759123613 No Hit TATCAACGCAGAGTACTTTTTTTTT 5937 0.3014966666260405 No Hit GTCCTACAGTGGACATTTCTAAATT 5878 0.29850048954486547 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5571 0.2829102121902765 No Hit CTTTAGGACGTGAAATATGGCGAGG 5539 0.28128516699370704 No Hit CTGAAGGACCTGGAATATGGCGAGA 3035 0.15412538036214132 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2870 0.1457462410673297 No Hit GTCCTACAGTGTGCATTTCTCATTT 2624 0.1332537061187014 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2477 0.12578865474696016 No Hit CTGTAGGACCTGGAATATGGCGAGA 2313 0.1174602981145413 No Hit ATTTAGAAATGTCCACTGTAGGACG 2291 0.11634307954189976 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2181 0.11075698667869201 No Hit GAATATGGCAAGAAAACTGAAAATC 2101 0.10669437368726817 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2009 0.10202236874713078 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.07826623927978E-5 0.0 9 0.0 0.0 0.0 1.015653247855956E-4 0.0 10 0.0 0.0 0.0 1.015653247855956E-4 0.0 11 0.0 0.0 0.0 1.015653247855956E-4 0.0 12 0.0 0.0 0.0 1.015653247855956E-4 0.0 13 0.0 0.0 0.0 1.015653247855956E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1525 0.0 14.383649 1 TCCAACG 170 0.0 12.850227 18 TAGGACC 910 0.0 12.213878 4 TTATCCG 55 0.0030697146 12.090505 5 CCAACGA 120 1.0053554E-8 11.87219 19 AACCGTT 65 8.044462E-4 11.688949 7 ATTGCGA 75 2.079404E-4 11.397014 17 TTGCGAG 85 5.3357675E-5 11.17411 18 CGGTTTC 80 3.780779E-4 10.6847 13 ACGAAAT 225 0.0 10.552791 12 TCTACAC 290 0.0 10.482409 3 GACGTGG 1735 0.0 10.455209 7 CGAAATC 240 0.0 10.288971 13 CCACCTT 805 0.0 10.264391 13 AGGACCT 1870 0.0 10.261688 5 GATATAC 600 0.0 10.172784 1 GGCGAGG 1430 0.0 10.161931 19 ATTACCC 75 0.0026488416 10.132995 3 AATGCCG 225 0.0 10.131193 18 TGTAGGA 3390 0.0 10.032113 2 >>END_MODULE