FastQCFastQC Report
Thu 26 May 2016
SRR1511864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886530
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77850.4126624013400264No Hit
TATCAACGCAGAGTACTTTTTTTTT50560.26800527953438325No Hit
GGTATCAACGCAGAGTACTTTTTTT48970.2595771071756081No Hit
GTCCTACAGTGGACATTTCTAAATT44940.23821513572537942No Hit
CTGTAGGACGTGGAATATGGCAAGA39600.20990919836949318No Hit
GTCCTAAAGTGTGTATTTCTCATTT38490.20402537993034833No Hit
CTTTAGGACGTGAAATATGGCGAGG36210.19193969881210476No Hit
GTCCTACAGTGTGCATTTCTCATTT25220.13368459552723785No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22710.12037974482250481No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20360.10792301209098186No Hit
CTGTAGGACCTGGAATATGGCGAGA19240.10198618627851135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG751.6916601E-917.308881
CCAACGA1004.3655746E-1115.11433119
GCGAACC457.0635875E-414.69332111
CGTGTAG400.00548423214.1685610
GGTATCA12850.013.5994631
AGCGTAC602.6851217E-413.3145231
TAGGACC9850.013.138034
TCCAACG1800.013.1200818
CCGGTTT1001.554854E-712.27941812
CGCCCTA550.003201522412.02276416
TAAGACG550.003202793212.0221284
GCCGGTT1052.9308467E-711.69468511
GTCTAAG1757.2759576E-1211.4124481
TGCCGGT1105.351776E-711.16310910
GGACCTG16250.011.1604666
TACACCG855.642473E-511.112895
AGGACCT17300.011.0839845
GGCGAGG10750.010.896378519
GTCCCGG650.0073532910.7540371
GTAGGAC28400.010.7098523