FastQCFastQC Report
Thu 26 May 2016
SRR1511864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886530
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99860.5293316300297372No Hit
TATCAACGCAGAGTACTTTTTTTTT71150.3771474612118546No Hit
GGTATCAACGCAGAGTACTTTTTTT68940.36543283170689045No Hit
CTGTAGGACGTGGAATATGGCAAGA41370.21929150344812964No Hit
GTCCTACAGTGGACATTTCTAAATT40890.21674714952849944No Hit
CTTTAGGACGTGAAATATGGCGAGG38980.20662274122330415No Hit
GTCCTAAAGTGTGTATTTCTCATTT37090.19660434766476018No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32360.17153186008173738No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24970.1323594111940971No Hit
GTCCTACAGTGTGCATTTCTCATTT23950.12695265911488288No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21600.11449592638335991No Hit
CTGAAGGACCTGGAATATGGCGAGA20340.10781699734433059No Hit
CTGTAGGACCTGGAATATGGCGAGA20240.10728692361107431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.014.7500761
TAGGACC8600.011.7089264
CTAGTAC1251.8331775E-811.3996343
AATCCCG2200.011.22512619
GGCGAGG11250.011.14453619
GCGCCAC600.005887137711.08062713
ACGAAAT1803.6379788E-1211.08062712
CGTGCGC700.001494699210.85449310
GTCCTAT1855.456968E-1210.8210161
TACACTG10900.010.5455335
AGGACCT16000.010.5090385
GTGCAAA1451.449007E-810.480211
GAACAGT4200.010.4022236
ACTGTTC6250.010.3334778
GACGTGA13150.010.3282777
GGACCTG15500.010.2942616
GTATCAA31700.010.2545271
TATGTCG1303.8546568E-710.228271516
TTAGGAC15500.010.1125793
CTAGACT1701.5606929E-910.05854
Produced by FastQC (version 0.11.5)