##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511864_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886530 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.05868870359867 31.0 30.0 34.0 26.0 34.0 2 30.27593571265763 31.0 30.0 34.0 26.0 34.0 3 30.361479541804265 31.0 30.0 34.0 26.0 34.0 4 33.97529591366159 37.0 35.0 37.0 30.0 37.0 5 33.52814161449858 35.0 35.0 37.0 28.0 37.0 6 33.46152194770293 36.0 35.0 37.0 28.0 37.0 7 33.266715080067634 36.0 35.0 37.0 27.0 37.0 8 33.15362808966727 36.0 35.0 37.0 27.0 37.0 9 34.52571387680026 38.0 35.0 39.0 27.0 39.0 10 34.28255421329107 38.0 34.0 39.0 27.0 39.0 11 34.26850036840124 38.0 34.0 39.0 27.0 39.0 12 34.059401122696165 37.0 34.0 39.0 26.0 39.0 13 34.03880881830663 37.0 34.0 39.0 26.0 39.0 14 34.87729058111984 38.0 34.0 40.0 25.0 41.0 15 34.80052901358579 38.0 34.0 40.0 25.0 41.0 16 34.66882424345226 38.0 34.0 40.0 25.0 41.0 17 34.620852040518834 38.0 34.0 40.0 25.0 41.0 18 34.53431273290115 38.0 34.0 40.0 24.0 41.0 19 34.56595336411295 39.0 34.0 40.0 24.0 41.0 20 34.41044828335622 38.0 34.0 40.0 23.0 41.0 21 34.26779112974615 38.0 34.0 40.0 21.0 41.0 22 34.347844985237444 39.0 34.0 40.0 22.0 41.0 23 34.211364780841016 38.0 34.0 40.0 19.0 41.0 24 34.04017693861216 38.0 34.0 40.0 18.0 41.0 25 33.549909092354746 38.0 33.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4470773516569544 1101 2 0.3303541222225981 1101 3 0.48666287205433534 1101 4 0.4759938879772392 1101 5 0.6460236261438723 1101 6 0.6669453815906508 1101 7 0.7180037776022488 1101 8 0.6681954622525836 1101 9 0.6604125846040958 1101 10 0.533991495580409 1101 11 0.27422815743872064 1101 12 0.4969712790480685 1101 13 0.4117098433594677 1101 14 0.45751014172917337 1101 15 0.2571598615366213 1101 16 0.4240444634050249 1101 17 0.5003945680388142 1101 18 0.6094005578610862 1101 19 0.4946344877683231 1101 20 0.5121974401204525 1101 21 0.5000237237184493 1101 22 0.2966750114360508 1101 23 0.39441471144343154 1101 24 0.3807727507481147 1101 25 0.25579050089533695 1102 1 0.6368889316830426 1102 2 0.6750654673825913 1102 3 0.7263366092200556 1102 4 0.7495237071450589 1102 5 0.8124724042517997 1102 6 0.7223672211952561 1102 7 0.8611280605006684 1102 8 0.8934956029761025 1102 9 0.8878358363472074 1102 10 0.9652710990048448 1102 11 1.0754862110411452 1102 12 1.2834052493147041 1102 13 1.3123468379560776 1102 14 1.3706219458341806 1102 15 1.3578464403368926 1102 16 1.2905241320428047 1102 17 1.3600271423476968 1102 18 1.4636806123853248 1102 19 1.3718299218538164 1102 20 1.3544825915821121 1102 21 1.5213204149994795 1102 22 1.2260867797472699 1102 23 1.4984355061636023 1102 24 1.5571331598760239 1102 25 1.5783231811146123 1103 1 0.49506982599198324 1103 2 0.5406552548594163 1103 3 0.6038790425391269 1103 4 0.652833684323781 1103 5 0.7255705714176841 1103 6 0.6312505830551913 1103 7 0.7511170064427546 1103 8 0.5459818745493479 1103 9 0.8812863070361274 1103 10 0.8798160997180133 1103 11 0.7635460455868639 1103 12 0.9027864003630555 1103 13 0.7109071360590562 1103 14 0.935897940699391 1103 15 0.9640670222795507 1103 16 0.9700250948155897 1103 17 0.9413440015689218 1103 18 1.1087669053535194 1103 19 1.3113321326653988 1103 20 1.2193654542265264 1103 21 1.1285073355528894 1103 22 1.1360220600211264 1103 23 1.1678425094441636 1103 24 1.384290484080843 1103 25 1.2287332510047833 1104 1 0.4984274771693329 1104 2 0.5535887136858832 1104 3 0.5362743004885644 1104 4 0.6058330980266646 1104 5 0.5601493247484299 1104 6 0.55597826720998 1104 7 0.7333003882556213 1104 8 0.8554093334127231 1104 9 0.9288164062239659 1104 10 0.858970611011685 1104 11 0.8987041399650835 1104 12 0.979417846614659 1104 13 1.1840128498296565 1104 14 1.2278747557006469 1104 15 1.2529434186391128 1104 16 1.2463907908979053 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-1.2561977092933816 2315 16 -1.4666286475118184 2315 17 -1.5228721787552502 2315 18 -1.386079300279178 2315 19 -1.2604846394644014 2315 20 -1.3274589921414162 2315 21 -1.4313950665824535 2315 22 -1.1735352319543253 2315 23 -1.1587390752827176 2315 24 -1.1380881213289342 2315 25 -1.3211482429487091 2316 1 -1.6908257147816066 2316 2 -1.2625475044409065 2316 3 -1.1516272397964258 2316 4 -1.1476220929612637 2316 5 -1.0796893448178437 2316 6 -1.188002927661735 2316 7 -1.23637991398099 2316 8 -1.2305640727283063 2316 9 -1.5775582359547613 2316 10 -1.769112207746879 2316 11 -1.5512482387196158 2316 12 -1.5250776055497255 2316 13 -1.5274890206297798 2316 14 -1.7897980435331746 2316 15 -1.802846010487137 2316 16 -1.8397556393553884 2316 17 -1.7622283068814326 2316 18 -1.7477494779921372 2316 19 -1.5224816145629845 2316 20 -1.607648588641176 2316 21 -1.8616872936661295 2316 22 -1.8079542888332725 2316 23 -1.6652075028741393 2316 24 -1.473568594509942 2316 25 -1.5596752239890534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47099.0 3 1.0 4 892.0 5 6983.0 6 8387.0 7 6793.0 8 5541.0 9 5039.0 10 4543.0 11 4438.0 12 4045.0 13 3883.0 14 3799.0 15 3913.0 16 3906.0 17 4116.0 18 4522.0 19 4949.0 20 6231.0 21 7850.0 22 10052.0 23 12260.0 24 15001.0 25 17832.0 26 19975.0 27 23056.0 28 26671.0 29 32279.0 30 40257.0 31 51782.0 32 66463.0 33 86220.0 34 111260.0 35 149756.0 36 220079.0 37 372549.0 38 494108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.24299003753617 21.85308638427802 14.665925947210967 23.237997630974842 2 14.594149046132317 22.821423460003288 41.56345247623944 21.020975017624952 3 18.420698319136193 27.31650172539 31.458868928667975 22.80393102680583 4 12.574727144545806 19.51742087324347 39.72414962921342 28.183702352997305 5 11.908821757904292 39.44712374553568 36.80739359852051 11.836660898039515 6 29.08318447095991 40.19299984627862 18.741816986742858 11.981998696018616 7 25.867068109173985 33.04310029525107 23.965163554250395 17.124668041324547 8 23.324834484476792 39.25890391353437 21.51866124577929 15.897600356209548 9 25.748437607671228 15.437019289383153 21.769439128982842 37.04510397396278 10 13.94851923902615 30.77942041738006 35.874330119319595 19.397730224274195 11 32.49876757857018 23.307713102892613 26.48741339920383 17.70610591933338 12 22.32299512862239 26.1901480495937 33.56877441652134 17.918082405262574 13 28.339544030574654 23.465622068029663 26.89201867979836 21.302815221597324 14 19.482276984728575 24.36404403852576 29.518003954350053 26.635675022395617 15 21.566579911265656 33.59278675663785 24.527518777862 20.31311455423449 16 19.972987413915934 30.06925950741256 30.064064779319473 19.893688299352036 17 19.980069227629563 30.4504566585212 29.541857272346583 20.027616841502653 18 19.455910252013357 28.740696528594604 32.762372338164255 19.04102088122778 19 22.048947008528884 27.863166766497223 29.920594954758208 20.16729127021569 20 22.019262879466535 28.699623117575655 30.638897870693814 18.642216132263997 21 21.3009069561576 27.92089179604883 29.465579662131002 21.312621585662566 22 21.443814834643497 29.711374852241946 29.850996273581654 18.9938140395329 23 20.398350410542108 29.367675043598563 30.305587507222253 19.928387038637073 24 21.44654558138376 28.177428477676724 31.116617075876825 19.259408865062692 25 21.018994162288692 28.918695571279923 30.49593247273959 19.566377793691796 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 292.0 1 292.0 2 732.5 3 1173.0 4 1173.0 5 1173.0 6 2806.0 7 4439.0 8 4439.0 9 4439.0 10 6376.0 11 8313.0 12 8313.0 13 8313.0 14 12282.0 15 16251.0 16 16251.0 17 16251.0 18 26373.5 19 36496.0 20 36496.0 21 36496.0 22 55296.0 23 74096.0 24 74096.0 25 74096.0 26 102236.5 27 130377.0 28 130377.0 29 130377.0 30 161594.5 31 192812.0 32 192812.0 33 192812.0 34 208895.5 35 224979.0 36 224979.0 37 224979.0 38 230861.0 39 236743.0 40 236743.0 41 236743.0 42 237938.0 43 239133.0 44 239133.0 45 239133.0 46 234432.0 47 229731.0 48 229731.0 49 229731.0 50 211734.0 51 193737.0 52 193737.0 53 193737.0 54 162425.0 55 131113.0 56 131113.0 57 131113.0 58 107347.0 59 83581.0 60 83581.0 61 83581.0 62 65104.5 63 46628.0 64 46628.0 65 46628.0 66 34584.0 67 22540.0 68 22540.0 69 22540.0 70 15881.5 71 9223.0 72 9223.0 73 9223.0 74 6305.0 75 3387.0 76 3387.0 77 3387.0 78 2225.5 79 1064.0 80 1064.0 81 1064.0 82 693.0 83 322.0 84 322.0 85 322.0 86 202.0 87 82.0 88 82.0 89 82.0 90 49.0 91 16.0 92 16.0 93 16.0 94 9.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3856816483172809 2 0.0 3 0.0 4 0.0 5 0.024701435969743394 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.060147466512592E-4 17 0.0 18 1.5902211997688879E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004823670972632293 25 1.5902211997688879E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1886530.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.62470385294537 #Duplication Level Percentage of deduplicated Percentage of total 1 88.94333981437322 68.15257072968232 2 8.289043369422922 12.702909868125037 3 1.54014776679626 3.5404009956161575 4 0.46960391478970565 1.4393304359577996 5 0.1982371937860607 0.7594933133247922 6 0.11634662358299207 0.5349015345802127 7 0.07893804458537386 0.42340230023594094 8 0.05081923963166734 0.31152073494467 9 0.039577311755215436 0.27293398132851693 >10 0.2325016844995955 3.233820326198969 >50 0.021379025299026022 1.146475367466533 >100 0.01634337229597331 2.6106063631673457 >500 0.0018613195910194985 1.0960318231543764 >1k 0.0016545063031284403 2.508105787999079 >5k 2.0681328789105504E-4 1.2674964382181904 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9986 0.5293316300297372 No Hit TATCAACGCAGAGTACTTTTTTTTT 7115 0.3771474612118546 No Hit GGTATCAACGCAGAGTACTTTTTTT 6894 0.36543283170689045 No Hit CTGTAGGACGTGGAATATGGCAAGA 4137 0.21929150344812964 No Hit GTCCTACAGTGGACATTTCTAAATT 4089 0.21674714952849944 No Hit CTTTAGGACGTGAAATATGGCGAGG 3898 0.20662274122330415 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3709 0.19660434766476018 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3236 0.17153186008173738 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2497 0.1323594111940971 No Hit GTCCTACAGTGTGCATTTCTCATTT 2395 0.12695265911488288 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2160 0.11449592638335991 No Hit CTGAAGGACCTGGAATATGGCGAGA 2034 0.10781699734433059 No Hit CTGTAGGACCTGGAATATGGCGAGA 2024 0.10728692361107431 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0601474665125919E-4 0.0 10 0.0 0.0 0.0 1.0601474665125919E-4 0.0 11 0.0 0.0 0.0 1.0601474665125919E-4 0.0 12 0.0 0.0 0.0 1.5902211997688879E-4 0.0 13 0.0 0.0 0.0 1.5902211997688879E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1480 0.0 14.750076 1 TAGGACC 860 0.0 11.708926 4 CTAGTAC 125 1.8331775E-8 11.399634 3 AATCCCG 220 0.0 11.225126 19 GGCGAGG 1125 0.0 11.144536 19 GCGCCAC 60 0.0058871377 11.080627 13 ACGAAAT 180 3.6379788E-12 11.080627 12 CGTGCGC 70 0.0014946992 10.854493 10 GTCCTAT 185 5.456968E-12 10.821016 1 TACACTG 1090 0.0 10.545533 5 AGGACCT 1600 0.0 10.509038 5 GTGCAAA 145 1.449007E-8 10.4802 11 GAACAGT 420 0.0 10.402223 6 ACTGTTC 625 0.0 10.333477 8 GACGTGA 1315 0.0 10.328277 7 GGACCTG 1550 0.0 10.294261 6 GTATCAA 3170 0.0 10.254527 1 TATGTCG 130 3.8546568E-7 10.2282715 16 TTAGGAC 1550 0.0 10.112579 3 CTAGACT 170 1.5606929E-9 10.0585 4 >>END_MODULE