FastQCFastQC Report
Thu 26 May 2016
SRR1511863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2322300
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77170.3322998751237997No Hit
TATCAACGCAGAGTACTTTTTTTTT50170.21603582655126383No Hit
GGTATCAACGCAGAGTACTTTTTTT49710.21405503164965764No Hit
GTCCTACAGTGGACATTTCTAAATT34590.1489471644490376No Hit
CTGTAGGACGTGGAATATGGCAAGA32010.13783748869655083No Hit
GTCCTAAAGTGTGTATTTCTCATTT29880.12866554708693967No Hit
CTTTAGGACGTGAAATATGGCGAGG29060.12513456487103303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.012.8571571
CCGTCCC604.298559E-412.593429
TAGGACC8650.012.3389064
CGTGCGC854.2098945E-612.22302510
CGTTAGG802.3621052E-411.2416851
GGCGAGG9300.011.17206419
TCCAACG2150.010.98310718
CGGTAGG1103.304116E-610.9010281
CCAACGA1409.14406E-910.79482519
CTACGGC700.001565531810.7945933
CCCGTCC700.001565812910.794368
TGGCGAG21700.010.7077718
GTCCTAT3500.010.5636151
GTATCAA29600.010.2626781
GTAGGAC23950.010.25373653
GTGGTAT5300.010.1811491
TGTAGGA23050.010.144342
AGGACGT21450.010.1277935
CAAGTCG1401.0548138E-710.119936
ATAGGAC2350.010.0481583
Produced by FastQC (version 0.11.5)