##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511863_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2322300 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.69319984498127 31.0 30.0 34.0 25.0 34.0 2 26.931493777720362 31.0 27.0 34.0 10.0 34.0 3 28.686131421435647 31.0 28.0 34.0 16.0 34.0 4 33.0983003918529 35.0 33.0 37.0 28.0 37.0 5 33.46669379494467 35.0 35.0 37.0 28.0 37.0 6 33.67777031391293 37.0 35.0 37.0 29.0 37.0 7 33.66976531886492 37.0 35.0 37.0 28.0 37.0 8 33.68939069026396 37.0 35.0 37.0 29.0 37.0 9 35.26040261809413 39.0 35.0 39.0 29.0 39.0 10 35.14182190070189 39.0 35.0 39.0 29.0 39.0 11 35.14849847134307 39.0 35.0 39.0 29.0 39.0 12 35.0318994100676 39.0 35.0 39.0 28.0 39.0 13 35.02798432588382 39.0 35.0 39.0 28.0 39.0 14 36.13847909400164 40.0 36.0 41.0 27.0 41.0 15 36.07469534513198 40.0 36.0 41.0 27.0 41.0 16 36.02707918873531 40.0 36.0 41.0 27.0 41.0 17 35.989495327907676 40.0 36.0 41.0 27.0 41.0 18 35.96007191146708 40.0 36.0 41.0 27.0 41.0 19 35.99318262067778 40.0 36.0 41.0 27.0 41.0 20 35.9295758515265 40.0 36.0 41.0 27.0 41.0 21 35.828892046677865 40.0 36.0 41.0 27.0 41.0 22 35.73341773242044 40.0 36.0 41.0 27.0 41.0 23 35.6286229169358 40.0 36.0 41.0 27.0 41.0 24 35.51368557033975 40.0 36.0 41.0 27.0 41.0 25 35.41281617362098 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8123749566410758 1101 2 3.4360820801317864 1101 3 2.024876931607306 1101 4 1.1058948276452156 1101 5 0.4633668256274177 1101 6 0.5785196603206515 1101 7 0.3892526574215438 1101 8 0.4493108424417329 1101 9 0.5021232727534013 1101 10 0.4046306581114649 1101 11 0.44130108891808106 1101 12 0.40545039721640563 1101 13 0.27221546262602914 1101 14 0.40859280237000917 1101 15 0.49333163593770735 1101 16 0.4583168823272814 1101 17 0.5828608398452175 1101 18 0.5403508432397146 1101 19 0.5625449179533035 1101 20 0.43883393197282317 1101 21 0.4963608675674678 1101 22 0.4868168657380991 1101 23 0.4251614692570911 1101 24 0.46262743906129344 1101 25 0.6029668932201702 1102 1 1.1512946490840363 1102 2 3.8901838126074075 1102 3 2.1569824592004103 1102 4 1.2938881704887777 1102 5 0.9164601155367151 1102 6 0.8078241579386045 1102 7 0.6799842268775791 1102 8 0.6733526425655469 1102 9 0.6721629285909714 1102 10 0.7107591448195834 1102 11 0.6768948947121416 1102 12 0.8342340755196389 1102 13 0.8708851528260126 1102 14 0.9340914162100731 1102 15 0.9789635723448171 1102 16 1.0304537218826297 1102 17 0.9863410433281175 1102 18 1.0575762355364375 1102 19 1.0392412816386027 1102 20 1.1410511149728535 1102 21 0.8716161183771831 1102 22 0.9808622097659452 1102 23 1.0526359217463437 1102 24 0.7038203889352417 1102 25 0.8820737845434365 1103 1 0.9149623895350274 1103 2 3.3646085710059346 1103 3 1.9252981517912993 1103 4 1.0974546684179032 1103 5 0.671510736797643 1103 6 0.6047178532906585 1103 7 0.6548806833893153 1103 8 0.3372619844891531 1103 9 0.4134652755767121 1103 10 0.7538217311058233 1103 11 0.7103279365897279 1103 12 0.5900555205265405 1103 13 0.7232206337279905 1103 14 0.8678926127126019 1103 15 0.576593576946749 1103 16 0.6486773758172859 1103 17 0.6621348767381505 1103 18 0.8224289736864563 1103 19 0.8327525563739897 1103 20 0.8235292557919962 1103 21 0.8775871262004671 1103 22 0.7615340919564701 1103 23 0.9340326083547197 1103 24 0.8654057547888954 1103 25 0.8913581839912084 1104 1 1.0776542495279884 1104 2 3.6507803931667517 1104 3 2.0277270093609623 1104 4 1.2637354987330482 1104 5 0.9434155340284676 1104 6 0.7973993542853322 1104 7 0.6959304613414048 1104 8 0.7590947357444549 1104 9 0.7860986742849008 1104 10 0.8451350714829147 1104 11 0.8585043871336282 1104 12 0.7924298037605269 1104 13 0.8148945656073963 1104 14 1.0985232180248516 1104 15 0.834912856854821 1104 16 0.9189558950405328 1104 17 0.8700283797058432 1104 18 0.7881442642746492 1104 19 0.8499782914072256 1104 20 0.7687648088329411 1104 21 0.9673479600120061 1104 22 0.767586036557212 1104 23 1.0107945834538654 1104 24 1.0765053191654346 1104 25 0.9871455453520426 1105 1 1.176013628584112 1105 2 3.866703404940335 1105 3 2.182147740887036 1105 4 1.4568625747253137 1105 5 1.0884101063775233 1105 6 0.9290326709862882 1105 7 0.7088414934432166 1105 8 0.7383374882748868 1105 9 0.9444913603716856 1105 10 0.7859389979395317 1105 11 0.8035822666916914 1105 12 0.6585392263131595 1105 13 0.7634749544879895 1105 14 0.9106513922296529 1105 15 0.9936424179542414 1105 16 0.9600978458480611 1105 17 1.0264416278507582 1105 18 1.041711275254002 1105 19 0.913933616232022 1105 20 0.9944429283252845 1105 21 1.1398166712589912 1105 22 1.0409498048965915 1105 23 1.1719314137612002 1105 24 1.202065572035707 1105 25 1.204731530717396 1106 1 0.9462454603313759 1106 2 3.6956753330537744 1106 3 2.114628740677542 1106 4 1.0442772755471381 1106 5 0.871177096668788 1106 6 0.6483039318171748 1106 7 0.6774623856030786 1106 8 0.5944377317660496 1106 9 0.5786639197076298 1106 10 0.6768427656991065 1106 11 0.47395825276220904 1106 12 0.5285333193343362 1106 13 0.45416648312481556 1106 14 0.6680720343732034 1106 15 0.6178061683363012 1106 16 0.6499878970533217 1106 17 0.4502570553683469 1106 18 0.6294633950695072 1106 19 0.45591223090811184 1106 20 0.5060426721272435 1106 21 0.6217776336844238 1106 22 0.5382680536834314 1106 23 0.5739210696796846 1106 24 0.7018565096312201 1106 25 0.5823325761152773 1107 1 1.000670606329777 1107 2 3.475124504105434 1107 3 2.0056138014626086 1107 4 1.228603196395511 1107 5 0.929683141573797 1107 6 0.7524945395334228 1107 7 0.6933710030368658 1107 8 0.7541547737611651 1107 9 0.7610409551168686 1107 10 0.6963450606797181 1107 11 0.7284020475781787 1107 12 0.6407840804077054 1107 13 0.7428871406384374 1107 14 1.0132650076331515 1107 15 0.9384692262047594 1107 16 0.9899919628315672 1107 17 0.9632205036859176 1107 18 0.8111600006486981 1107 19 0.9082477012951387 1107 20 0.9330224709216282 1107 21 0.8167728622752506 1107 22 1.015774387561109 1107 23 0.8716301212445003 1107 24 1.073850857863448 1107 25 1.044156262465684 1108 1 0.8687344095516956 1108 2 3.589440422717825 1108 3 1.977555784295859 1108 4 1.1658864912276528 1108 5 0.8702325122859165 1108 6 0.7636587982440446 1108 7 0.6495111821870552 1108 8 0.6358136314285616 1108 9 0.5433406292706024 1108 10 0.622832794677791 1108 11 0.7112429136592766 1108 12 0.7148782147398904 1108 13 0.6536089618971914 1108 14 0.8564596496590511 1108 15 0.8049723171763361 1108 16 0.668706022945635 1108 17 0.7359568727405303 1108 18 0.7936593589621239 1108 19 0.6768424729634788 1108 20 0.5216219845449572 1108 21 0.5689883123476847 1108 22 0.4839260692149594 1108 23 0.5877186174357121 1108 24 0.7065353264382068 1108 25 0.7443494209992494 1109 1 0.5545825988952018 1109 2 2.6345032168112716 1109 3 1.4622741935890282 1109 4 0.9146330726943503 1109 5 0.6022944499771512 1109 6 0.45949345496511995 1109 7 0.41126627211976796 1109 8 0.33008695266534716 1109 9 0.2771892714485631 1109 10 0.34903146950806274 1109 11 0.4092494986270623 1109 12 0.4583847048430485 1109 13 0.36513663878302793 1109 14 0.43045563075321525 1109 15 0.3499302884779567 1109 16 0.39295813853163253 1109 17 0.3733533471307524 1109 18 0.3221517661231985 1109 19 0.4108200471753918 1109 20 0.4251776784665964 1109 21 0.4655743388405966 1109 22 0.48823271361726484 1109 23 0.27162181987682743 1109 24 0.34492447266705284 1109 25 0.3300478185732274 1110 1 0.6660601879497712 1110 2 3.2323244580108437 1110 3 1.7015730807550398 1110 4 0.9164119997469626 1110 5 0.6735496619402355 1110 6 0.4422215650650969 1110 7 0.2905906902950761 1110 8 0.3128507620285177 1110 9 0.39252238092854697 1110 10 0.2436312057474197 1110 11 0.2439448364585033 1110 12 0.21544121608685174 1110 13 0.26967335978673646 1110 14 0.32309242489729684 1110 15 0.4101378719327471 1110 16 0.35496325749554813 1110 17 0.3256313654042913 1110 18 0.39699811701177623 1110 19 0.35130799574223914 1110 20 0.35093097172362775 1110 21 0.3799997557502124 1110 22 0.5998224018074865 1110 23 0.45459054645996133 1110 24 0.31085168169693134 1110 25 0.4062883377888298 1111 1 -0.194956965241726 1111 2 2.483927315930554 1111 3 0.9671991725906537 1111 4 0.2136711421081401 1111 5 -0.19121856276360205 1111 6 -0.4406843625515151 1111 7 -0.5378851781055687 1111 8 -0.7085550967484053 1111 9 -0.8930983559398484 1111 10 -0.9286264664192743 1111 11 -0.9571461170369986 1111 12 -0.7817422372028204 1111 13 -0.7366801289328961 1111 14 -1.0974044731653052 1111 15 -1.1132152162021285 1111 16 -1.0835539008848727 1111 17 -1.1296069202589933 1111 18 -1.2382084055320135 1111 19 -1.1425097499549324 1111 20 -1.0615042445886829 1111 21 -1.0993488546911436 1111 22 -1.0084643407742604 1111 23 -1.208074539084599 1111 24 -1.1194109400092316 1111 25 -1.312430420578302 1112 1 0.3333445829862107 1112 2 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-0.8169109422804937 2315 15 -0.8068756572149312 2315 16 -0.847156060237765 2315 17 -0.6115292958452869 2315 18 -0.7212928120826163 2315 19 -0.7767445112634661 2315 20 -0.771802658612053 2315 21 -1.063260060868103 2315 22 -0.8744612403688237 2315 23 -0.739780407993095 2315 24 -0.8960364169946828 2315 25 -0.9839035851780338 2316 1 -1.5249362650683125 2316 2 -1.578071818244883 2316 3 -0.9387195344446688 2316 4 -1.1535037818212075 2316 5 -1.4125412341806936 2316 6 -1.3956204333500892 2316 7 -1.463340295800485 2316 8 -1.6753545812344726 2316 9 -1.7038852113297267 2316 10 -1.8002965872615135 2316 11 -1.911254305299103 2316 12 -1.6704365090767084 2316 13 -1.6122539243047171 2316 14 -1.8741249884801974 2316 15 -2.290385189008269 2316 16 -2.063284151750075 2316 17 -1.817668742123054 2316 18 -1.990598131080553 2316 19 -1.828126488234581 2316 20 -2.0222218091336615 2316 21 -1.9882670467101278 2316 22 -2.0616285812246886 2316 23 -2.118039358741804 2316 24 -1.8924137589150547 2316 25 -1.8322803194410398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76301.0 3 1.0 4 717.0 5 3495.0 6 4232.0 7 3698.0 8 3566.0 9 3399.0 10 3311.0 11 3121.0 12 3109.0 13 3089.0 14 3140.0 15 3250.0 16 3485.0 17 3624.0 18 4174.0 19 5136.0 20 6753.0 21 8406.0 22 10787.0 23 12940.0 24 14774.0 25 17148.0 26 19631.0 27 22306.0 28 25880.0 29 31526.0 30 39466.0 31 50866.0 32 66000.0 33 87470.0 34 119784.0 35 173378.0 36 268181.0 37 364417.0 38 851739.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.687809499203375 19.432459199931102 13.32993153339362 24.549799767471903 2 15.744070030334878 21.532432899060343 40.6100594807766 22.11343758982818 3 18.377987340136933 25.457778926064677 30.109977177797877 26.054256556000517 4 11.813934461525212 17.462817034836153 39.61637170046936 31.10687680316927 5 12.646649869977086 38.26600806189426 36.25867303855731 12.828669029571332 6 30.244243778716513 38.23935201333178 18.824124671119208 12.692279536832496 7 26.796882401067908 32.1048098867502 23.137880549455282 17.960427162726607 8 24.039228351203548 37.668475218533345 21.285578951901133 17.006717478361967 9 25.836024630753997 15.631830512853636 21.075485510054687 37.45665934633769 10 14.383585238771907 29.652801102355426 35.27115359772639 20.692460061146278 11 33.14287559746803 22.970072772682254 25.57158851138957 18.315463118460148 12 22.619687378891616 25.225250828919606 32.78788270249322 19.36717908969556 13 28.314976484458903 22.661730168531633 26.474942449147264 22.5483508978622 14 20.295698230202817 22.96115919562503 28.756620591654823 27.98652198251733 15 22.30443956422512 31.65654738836498 24.253800111957975 21.785212935451923 16 21.16350170089997 28.96658485122508 28.77139043189941 21.09852301597554 17 20.861972597595983 29.143668415237105 28.710645323255875 21.283713663911037 18 20.491710803944365 28.260862076389788 30.90772079404039 20.339706325625457 19 22.552811086657247 27.455994887798106 28.70153430648322 21.289659719061422 20 22.55832419718914 27.94510390228905 29.395877668392846 20.100694232128973 21 22.299498077744552 27.235870733488582 28.325105758498577 22.13952543026829 22 22.128368558242645 28.52591086021584 28.72542226612746 20.620298315414054 23 21.62432281486821 28.141964615206412 28.8180194006193 21.41569316930608 24 22.03825265562103 27.390360358343052 29.70045352684731 20.870933459188613 25 21.925281986049903 27.816221830461295 29.178978571902242 21.079517611586553 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 317.0 1 317.0 2 573.5 3 830.0 4 830.0 5 830.0 6 2294.5 7 3759.0 8 3759.0 9 3759.0 10 5344.5 11 6930.0 12 6930.0 13 6930.0 14 10765.0 15 14600.0 16 14600.0 17 14600.0 18 25121.5 19 35643.0 20 35643.0 21 35643.0 22 54232.5 23 72822.0 24 72822.0 25 72822.0 26 101940.0 27 131058.0 28 131058.0 29 131058.0 30 166421.0 31 201784.0 32 201784.0 33 201784.0 34 223114.0 35 244444.0 36 244444.0 37 244444.0 38 258302.0 39 272160.0 40 272160.0 41 272160.0 42 281371.5 43 290583.0 44 290583.0 45 290583.0 46 292491.5 47 294400.0 48 294400.0 49 294400.0 50 280090.0 51 265780.0 52 265780.0 53 265780.0 54 231903.5 55 198027.0 56 198027.0 57 198027.0 58 166887.0 59 135747.0 60 135747.0 61 135747.0 62 108855.5 63 81964.0 64 81964.0 65 81964.0 66 62059.0 67 42154.0 68 42154.0 69 42154.0 70 30350.0 71 18546.0 72 18546.0 73 18546.0 74 12937.0 75 7328.0 76 7328.0 77 7328.0 78 4886.5 79 2445.0 80 2445.0 81 2445.0 82 1579.0 83 713.0 84 713.0 85 713.0 86 445.5 87 178.0 88 178.0 89 178.0 90 119.0 91 60.0 92 60.0 93 60.0 94 42.5 95 25.0 96 25.0 97 25.0 98 14.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.47461568272833 3 0.0 4 0.0 5 7.320328984196701E-4 6 0.001291822761917065 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.58364552383413E-4 14 0.0 15 0.0 16 0.0 17 9.473366920725142E-4 18 0.0 19 5.597898634973948E-4 20 9.473366920725142E-4 21 0.0012057012444559273 22 0.005468716358782242 23 1.291822761917065E-4 24 0.0024114024889118546 25 9.473366920725142E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2322300.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.56722384584779 #Duplication Level Percentage of deduplicated Percentage of total 1 86.79668107661402 66.45780909069768 2 9.591999232209611 14.68865504683587 3 1.9995244354342898 4.592941050994191 4 0.6591046837544472 2.018632634354939 5 0.29145723763883824 1.1158035777892692 6 0.15957411145505235 0.7330888027068755 7 0.10102829816970609 0.5414819424507458 8 0.07354432524178238 0.45048678507035084 9 0.04860660114253999 0.3349505259060031 >10 0.24757195147782882 3.2956741991718013 >50 0.015969965247972954 0.836853657598006 >100 0.012101646680687705 1.8545606043273906 >500 0.0015316748639735156 0.7839255227644886 >1k 0.0011913026719778358 1.7420277601927672 >5k 1.1345739733122247E-4 0.5531087991396474 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7717 0.3322998751237997 No Hit TATCAACGCAGAGTACTTTTTTTTT 5017 0.21603582655126383 No Hit GGTATCAACGCAGAGTACTTTTTTT 4971 0.21405503164965764 No Hit GTCCTACAGTGGACATTTCTAAATT 3459 0.1489471644490376 No Hit CTGTAGGACGTGGAATATGGCAAGA 3201 0.13783748869655083 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2988 0.12866554708693967 No Hit CTTTAGGACGTGAAATATGGCGAGG 2906 0.12513456487103303 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.612151746113767E-5 0.0 10 0.0 0.0 0.0 8.612151746113767E-5 0.0 11 0.0 0.0 0.0 8.612151746113767E-5 0.0 12 0.0 0.0 0.0 8.612151746113767E-5 8.612151746113767E-5 13 0.0 0.0 0.0 1.291822761917065E-4 8.612151746113767E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1500 0.0 12.857157 1 CCGTCCC 60 4.298559E-4 12.59342 9 TAGGACC 865 0.0 12.338906 4 CGTGCGC 85 4.2098945E-6 12.223025 10 CGTTAGG 80 2.3621052E-4 11.241685 1 GGCGAGG 930 0.0 11.172064 19 TCCAACG 215 0.0 10.983107 18 CGGTAGG 110 3.304116E-6 10.901028 1 CCAACGA 140 9.14406E-9 10.794825 19 CTACGGC 70 0.0015655318 10.794593 3 CCCGTCC 70 0.0015658129 10.79436 8 TGGCGAG 2170 0.0 10.70777 18 GTCCTAT 350 0.0 10.563615 1 GTATCAA 2960 0.0 10.262678 1 GTAGGAC 2395 0.0 10.2537365 3 GTGGTAT 530 0.0 10.181149 1 TGTAGGA 2305 0.0 10.14434 2 AGGACGT 2145 0.0 10.127793 5 CAAGTCG 140 1.0548138E-7 10.11993 6 ATAGGAC 235 0.0 10.048158 3 >>END_MODULE