FastQCFastQC Report
Thu 26 May 2016
SRR1511863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2322300
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT100460.4325883822072945No Hit
TATCAACGCAGAGTACTTTTTTTTT66810.2876889290789304No Hit
GGTATCAACGCAGAGTACTTTTTTT65890.283727339275718No Hit
GTCCTACAGTGGACATTTCTAAATT36990.1592817465443741No Hit
CTGTAGGACGTGGAATATGGCAAGA35050.15092795935064376No Hit
GTCCTAAAGTGTGTATTTCTCATTT32510.1399905266330793No Hit
CTTTAGGACGTGAAATATGGCGAGG32470.139818283598157No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32310.1391293114584679No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23490.1011497222581062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16900.014.2812071
TCCAACG2400.013.45452118
GGACCGT500.001503386113.2959466
TAGGACC8100.012.549614
GGCGAGG9950.012.50399419
CCAACGA1600.012.46521919
ATAGGAC3200.011.5783363
GCGTGCG600.00589049311.0799549
TTAGGAC15100.011.0101343
CGCTGAC700.001495682210.85383312
TATGTCG1053.4807072E-610.85383216
TGAGTTA7300.010.79807712
AGGACGT20950.010.7925585
TGGCGAG20800.010.63881218
GACGTGA11050.010.5714387
TGGTATC4700.010.5108292
GGACGTG21050.010.4671166
TAGGACG22800.010.3751894
TGTCGAG1106.0687E-610.360718
GTGGTAT4800.010.3345891
Produced by FastQC (version 0.11.5)