##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511863_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2322300 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0112177582569 31.0 31.0 34.0 28.0 34.0 2 31.207571373207596 31.0 31.0 34.0 28.0 34.0 3 31.31823881496792 33.0 31.0 34.0 28.0 34.0 4 34.896377298367995 37.0 35.0 37.0 32.0 37.0 5 34.543595573354004 37.0 35.0 37.0 31.0 37.0 6 34.49059596090083 37.0 35.0 37.0 30.0 37.0 7 34.34624854669939 37.0 35.0 37.0 30.0 37.0 8 34.26304008956638 37.0 35.0 37.0 30.0 37.0 9 35.78233647676872 39.0 35.0 39.0 30.0 39.0 10 35.60914309090126 39.0 35.0 39.0 30.0 39.0 11 35.583708823149465 39.0 35.0 39.0 30.0 39.0 12 35.4223437970977 39.0 35.0 39.0 30.0 39.0 13 35.40813417732421 39.0 35.0 39.0 30.0 39.0 14 36.454734099814836 40.0 36.0 41.0 30.0 41.0 15 36.393456056495715 40.0 36.0 41.0 29.0 41.0 16 36.2967196313999 40.0 36.0 41.0 29.0 41.0 17 36.25331223356156 40.0 36.0 41.0 29.0 41.0 18 36.186075873056886 40.0 36.0 41.0 29.0 41.0 19 36.21161348662964 40.0 36.0 41.0 29.0 41.0 20 36.08208586315291 40.0 36.0 41.0 28.0 41.0 21 35.97550273435818 39.0 36.0 41.0 27.0 41.0 22 36.05882056581837 40.0 36.0 41.0 29.0 41.0 23 35.956438875252985 40.0 36.0 41.0 28.0 41.0 24 35.839609008310724 40.0 36.0 41.0 27.0 41.0 25 35.409833354863714 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29269849437320516 1101 2 0.2804621273528731 1101 3 0.38483422741773055 1101 4 0.2544116714587048 1101 5 0.40136698828666084 1101 6 0.3249419215740872 1101 7 0.26637477150162425 1101 8 0.2404224060374105 1101 9 0.12407218073297344 1101 10 0.08376824258412086 1101 11 -0.052377949909867993 1101 12 0.27047619034522086 1101 13 0.07153351337890257 1101 14 0.17616507800412506 1101 15 -0.05696414812063466 1101 16 0.1143323457799923 1101 17 0.2711828042413913 1101 18 0.26942075068481586 1101 19 0.22371870306466946 1101 20 0.2872785371117317 1101 21 0.22009520301393337 1101 22 0.0177415709537172 1101 23 -0.15415393733066907 1101 24 -0.22593976436680663 1101 25 -0.13569701630535036 1102 1 0.38051912796941423 1102 2 0.467735250205223 1102 3 0.45760605382923814 1102 4 0.46756628436569514 1102 5 0.47310323210725613 1102 6 0.502432299201736 1102 7 0.5097357831740794 1102 8 0.5902559771263327 1102 9 0.5092866990760356 1102 10 0.6324834652220446 1102 11 0.6608260084963646 1102 12 0.7200318565543142 1102 13 0.8298881549959987 1102 14 1.0469101501831304 1102 15 1.1350281988410273 1102 16 0.8905630673906302 1102 17 1.018645324091743 1102 18 1.0274333382730774 1102 19 1.196027130378745 1102 20 1.0284827018718659 1102 21 1.0824986428438237 1102 22 0.7500500879868852 1102 23 0.9260369848918515 1102 24 0.8614634511653634 1102 25 0.9085558031170322 1103 1 0.44135667974115833 1103 2 0.46086686548363787 1103 3 0.49694107453793634 1103 4 0.46756628436569514 1103 5 0.5239315800133824 1103 6 0.5934125108906443 1103 7 0.620906975074675 1103 8 0.49789518559389023 1103 9 0.618640127515981 1103 10 0.8516505153831133 1103 11 0.7528646647319803 1103 12 0.6951008855464949 1103 13 0.5440390983922256 1103 14 0.9357159486184727 1103 15 0.9463834680540941 1103 16 0.7072680834973895 1103 17 0.7443816333416251 1103 18 0.9117982715450523 1103 19 0.9646994727625469 1103 20 0.7836023521249729 1103 21 0.6925423612055397 1103 22 0.886900064977219 1103 23 1.0383241454993026 1103 24 0.9483824571478721 1103 25 0.998466065427202 1104 1 0.3373982529450572 1104 2 0.33277091880075815 1104 3 0.36650985876184095 1104 4 0.31292047097768716 1104 5 0.4501274884466966 1104 6 0.4253584796119654 1104 7 0.4321673901247962 1104 8 0.44238889136072856 1104 9 0.5986011588366082 1104 10 0.5185197607715182 1104 11 0.5924371953879373 1104 12 0.6340144119846727 1104 13 0.6697603072271932 1104 14 0.6976385605160118 1104 15 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0.989373571175733 1107 15 0.9096127134563332 1107 16 0.9536533010400561 1107 17 1.1718527083261066 1107 18 1.202092803193075 1107 19 1.2072753788227146 1107 20 1.0300183684407713 1107 21 0.9882527080831807 1107 22 0.9420084091000618 1107 23 0.9119593624458631 1107 24 1.0151400894812639 1107 25 0.9258391804429351 1108 1 0.14890307676779102 1108 2 0.1174085071797002 1108 3 0.14813446661063168 1108 4 0.13011400865002543 1108 5 0.21594577761364775 1108 6 0.23905286539788762 1108 7 0.2828505749298529 1108 8 0.2561669762867069 1108 9 0.27845760350073334 1108 10 0.14513733148314856 1108 11 0.21284349815940118 1108 12 0.2262273003703612 1108 13 0.3916699144180811 1108 14 0.4031689623435142 1108 15 0.4411499769567655 1108 16 0.45534452581307505 1108 17 0.33107146036536506 1108 18 0.42184543845842626 1108 19 0.3463538734042686 1108 20 0.2761519349564807 1108 21 0.39380949625700623 1108 22 0.4404422588279431 1108 23 0.45075856022889127 1108 24 0.5347224059045246 1108 25 0.4934000754108112 1109 1 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-0.6973386385227229 2313 18 -0.5010267153950636 2313 19 -0.41428874796412885 2313 20 -0.5717640945044877 2313 21 -0.553867302409067 2313 22 -0.5188239670404755 2313 23 -0.5442108273957729 2313 24 -0.5375768629964384 2313 25 -0.43707910639680136 2314 1 -0.5736027609233645 2314 2 -0.6129513812226506 2314 3 -0.7254014373318931 2314 4 -0.5379365685765123 2314 5 -0.690723911099937 2314 6 -0.7105372110723565 2314 7 -0.7906414002893598 2314 8 -0.7791317779757065 2314 9 -0.813663733240972 2314 10 -0.8565230807925914 2314 11 -0.6993168136410688 2314 12 -0.9282940089548362 2314 13 -0.9649958020173344 2314 14 -0.9617178121510292 2314 15 -0.9352338167713938 2314 16 -1.0315673628260527 2314 17 -0.9415998889162722 2314 18 -1.0043734667817077 2314 19 -1.0093715814215827 2314 20 -0.8286032714539999 2314 21 -0.8726077736507278 2314 22 -0.921132570325156 2314 23 -0.9427206725450503 2314 24 -0.9411960860629094 2314 25 -0.8835075917091615 2315 1 -0.5139552655615844 2315 2 -0.5272206684830074 2315 3 -0.6229209565031972 2315 4 -0.5960708450143599 2315 5 -0.5548153999682128 2315 6 -0.5394758319752668 2315 7 -0.7161767280568299 2315 8 -0.6839999752546149 2315 9 -0.8511762950838886 2315 10 -0.8473500365749018 2315 11 -1.0882334030040894 2315 12 -0.8926029143351712 2315 13 -1.0752752547075062 2315 14 -1.0904438480199232 2315 15 -1.0368987441059616 2315 16 -1.2014208724117097 2315 17 -1.2512039009756393 2315 18 -1.223199804134829 2315 19 -1.1541062366473085 2315 20 -1.2068258673105277 2315 21 -1.3341820624571596 2315 22 -1.3882234407642429 2315 23 -1.262045618432495 2315 24 -1.1611317307753382 2315 25 -1.1951475886170186 2316 1 -1.8898334857020984 2316 2 -1.3659380900641587 2316 3 -1.0920108232621217 2316 4 -1.066141501816574 2316 5 -1.1493920579413839 2316 6 -1.1456584130553864 2316 7 -1.0784436448831727 2316 8 -1.1931270333058563 2316 9 -1.5304069431926095 2316 10 -1.397547746992899 2316 11 -1.3221338092917918 2316 12 -1.44424491987062 2316 13 -1.391743878297028 2316 14 -1.5760020057092277 2316 15 -1.6834256717548186 2316 16 -1.6549561520228053 2316 17 -1.7279726496450039 2316 18 -1.7566758482409952 2316 19 -1.6773785159390542 2316 20 -1.6949693409816646 2316 21 -1.8278892603139525 2316 22 -1.9415148812226874 2316 23 -1.9408471362452886 2316 24 -1.772155056834528 2316 25 -1.6868699202471333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32074.0 3 2.0 4 573.0 5 5271.0 6 6747.0 7 5450.0 8 4421.0 9 3901.0 10 3665.0 11 3521.0 12 3350.0 13 3267.0 14 3217.0 15 3376.0 16 3312.0 17 3515.0 18 3873.0 19 4399.0 20 5303.0 21 6891.0 22 8808.0 23 11039.0 24 13962.0 25 16261.0 26 18793.0 27 21363.0 28 25318.0 29 30748.0 30 38804.0 31 50458.0 32 65304.0 33 85465.0 34 111686.0 35 151615.0 36 224760.0 37 405584.0 38 936204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.60100754186418 20.201119260375037 13.990365575480034 24.20750762228075 2 14.511604874477888 21.373552081987686 41.38616888429574 22.728674159238686 3 18.306205055333074 25.944968350342336 30.594023166688196 25.154803427636395 4 12.211428325367093 18.169013478017483 39.27136890152004 30.34818929509538 5 12.63480123480666 38.38341461859817 36.21759267833907 12.764191468256106 6 30.163760065452355 38.73272187055936 18.527278990655816 12.57623907333247 7 26.70417258752099 32.22124617835767 23.151530809972872 17.923050424148474 8 24.087370279464324 37.84623003057314 21.086638246565904 16.979761443396633 9 25.840115402833398 15.46708004995048 20.977823709253755 37.714980837962365 10 14.301080825044139 29.935882530250186 35.209619773500414 20.553416871205272 11 33.23575765404986 22.995134134263445 25.426516815226286 18.342591396460406 12 22.49967704430952 25.50854756060802 32.731817594626015 19.259957800456444 13 28.389053955130688 22.695302071222496 26.43482754166128 22.48081643198553 14 20.31206131852043 23.022477716057356 28.738492012229255 27.926968953192954 15 22.309219308444213 31.79050940877578 24.259527192869136 21.640744089910864 16 21.10367450847157 29.137315339080228 28.7474857867017 21.0115243657465 17 20.819015631055418 29.297119235240928 28.66696809197778 21.216897041725876 18 20.458390586225118 28.229353756772923 30.949890604095337 20.362365052906625 19 22.494940360849156 27.465228437325067 28.876243379408344 21.163587822417433 20 22.572062179735607 27.8795590578306 29.505404125220686 20.042974637213106 21 22.188821427033545 27.213236877233776 28.509882444128664 22.088059251604015 22 22.09150411230246 28.530853033630454 28.92666752788184 20.450975326185247 23 21.55397666106877 28.18240537398269 28.95693924126943 21.306678723679113 24 21.978831180407795 27.432392453844194 29.812000659722354 20.776775706025653 25 21.875339103475003 27.897601515738707 29.28153124058046 20.94552814020583 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 271.0 1 271.0 2 615.0 3 959.0 4 959.0 5 959.0 6 2460.0 7 3961.0 8 3961.0 9 3961.0 10 5651.0 11 7341.0 12 7341.0 13 7341.0 14 11228.0 15 15115.0 16 15115.0 17 15115.0 18 25639.5 19 36164.0 20 36164.0 21 36164.0 22 55403.5 23 74643.0 24 74643.0 25 74643.0 26 104625.5 27 134608.0 28 134608.0 29 134608.0 30 168880.5 31 203153.0 32 203153.0 33 203153.0 34 224333.0 35 245513.0 36 245513.0 37 245513.0 38 259015.0 39 272517.0 40 272517.0 41 272517.0 42 282437.5 43 292358.0 44 292358.0 45 292358.0 46 292347.0 47 292336.0 48 292336.0 49 292336.0 50 277735.5 51 263135.0 52 263135.0 53 263135.0 54 229655.5 55 196176.0 56 196176.0 57 196176.0 58 164851.0 59 133526.0 60 133526.0 61 133526.0 62 107229.0 63 80932.0 64 80932.0 65 80932.0 66 61268.5 67 41605.0 68 41605.0 69 41605.0 70 29778.5 71 17952.0 72 17952.0 73 17952.0 74 12408.0 75 6864.0 76 6864.0 77 6864.0 78 4568.5 79 2273.0 80 2273.0 81 2273.0 82 1478.0 83 683.0 84 683.0 85 683.0 86 425.0 87 167.0 88 167.0 89 167.0 90 101.5 91 36.0 92 36.0 93 36.0 94 22.0 95 8.0 96 8.0 97 8.0 98 6.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40235972957843513 2 0.0 3 0.0 4 0.0 5 0.02760194634629462 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.291822761917065E-4 17 0.0 18 4.306075873056883E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00477974421909314 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2322300.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.5966638427006 #Duplication Level Percentage of deduplicated Percentage of total 1 85.87752762088687 64.06177059577533 2 10.155848859873574 15.151848868745263 3 2.1884174414709086 4.897459206867247 4 0.7023880372108884 2.0958321719581967 5 0.3253914959553441 1.2136560020527138 6 0.19064378167908555 0.8532834057369567 7 0.11158624152131331 0.5826772943765066 8 0.07968031537712143 0.4755108560853995 9 0.05424265530994811 0.364168901168251 >10 0.27865966600293696 3.5643520199582843 >50 0.018545397041316903 0.949439096933685 >100 0.013785835434494388 2.0238255355716936 >500 0.001900482873634865 1.0094430968001535 >1k 0.0012093981923103102 1.7550657034305102 >5k 1.1518078022002954E-4 0.5700859639322171 >10k+ 5.759039011001477E-5 0.43158128060761514 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10046 0.4325883822072945 No Hit TATCAACGCAGAGTACTTTTTTTTT 6681 0.2876889290789304 No Hit GGTATCAACGCAGAGTACTTTTTTT 6589 0.283727339275718 No Hit GTCCTACAGTGGACATTTCTAAATT 3699 0.1592817465443741 No Hit CTGTAGGACGTGGAATATGGCAAGA 3505 0.15092795935064376 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3251 0.1399905266330793 No Hit CTTTAGGACGTGAAATATGGCGAGG 3247 0.139818283598157 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3231 0.1391293114584679 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2349 0.1011497222581062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.3060758730568835E-5 0.0 5 0.0 0.0 0.0 4.3060758730568835E-5 0.0 6 0.0 0.0 0.0 4.3060758730568835E-5 0.0 7 0.0 0.0 0.0 4.3060758730568835E-5 0.0 8 0.0 0.0 0.0 4.3060758730568835E-5 0.0 9 0.0 0.0 0.0 4.3060758730568835E-5 0.0 10 0.0 0.0 0.0 4.3060758730568835E-5 0.0 11 0.0 0.0 0.0 8.612151746113767E-5 0.0 12 0.0 0.0 0.0 8.612151746113767E-5 8.612151746113767E-5 13 0.0 0.0 0.0 2.1530379365284415E-4 8.612151746113767E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1690 0.0 14.281207 1 TCCAACG 240 0.0 13.454521 18 GGACCGT 50 0.0015033861 13.295946 6 TAGGACC 810 0.0 12.54961 4 GGCGAGG 995 0.0 12.503994 19 CCAACGA 160 0.0 12.465219 19 ATAGGAC 320 0.0 11.578336 3 GCGTGCG 60 0.005890493 11.079954 9 TTAGGAC 1510 0.0 11.010134 3 CGCTGAC 70 0.0014956822 10.853833 12 TATGTCG 105 3.4807072E-6 10.853832 16 TGAGTTA 730 0.0 10.798077 12 AGGACGT 2095 0.0 10.792558 5 TGGCGAG 2080 0.0 10.638812 18 GACGTGA 1105 0.0 10.571438 7 TGGTATC 470 0.0 10.510829 2 GGACGTG 2105 0.0 10.467116 6 TAGGACG 2280 0.0 10.375189 4 TGTCGAG 110 6.0687E-6 10.3607 18 GTGGTAT 480 0.0 10.334589 1 >>END_MODULE