FastQCFastQC Report
Thu 26 May 2016
SRR1511862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347251
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73350.31249321014241765No Hit
GGTATCAACGCAGAGTACTTTTTTT46470.19797627096548262No Hit
TATCAACGCAGAGTACTTTTTTTTT46160.19665557709848672No Hit
GTCCTACAGTGGACATTTCTAAATT40890.17420378135955633No Hit
CTGTAGGACGTGGAATATGGCAAGA39300.1674298999127064No Hit
GTCCTAAAGTGTGTATTTCTCATTT32130.1368835288599302No Hit
CTTTAGGACGTGAAATATGGCGAGG29980.1277238778468941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG704.7353933E-714.8435147
GGTATCA13600.013.8781711
ATCGCGC707.707951E-613.4941048
GTATCGC852.895522E-713.335356
CGGTAGG602.6844072E-413.3152121
GCGCGCC802.133158E-612.98807511
CGCGCCT802.133158E-612.98807512
TGTATCG1251.1277734E-1012.8463875
TCGCGCG751.5688274E-512.5944979
ATACCGC550.003203407212.022026
GTCCTAA10650.011.1585241
GGCGAGG9600.010.9217919
TTAGGAC12600.010.8702523
TAGGACC7150.010.8330294
AGGACGT23000.010.7190125
GGACGTG22850.010.7067016
GTCCTAC22500.010.6965531
TCCAACG2400.010.62660718
GTATCAA28400.010.4786971
TCCTACA24500.010.2309742
Produced by FastQC (version 0.11.5)