##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511862_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2347251 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.865671374727288 31.0 30.0 34.0 25.0 34.0 2 27.10907781059631 31.0 27.0 34.0 10.0 34.0 3 28.86385265146335 31.0 28.0 34.0 16.0 34.0 4 33.28847617915596 35.0 33.0 37.0 28.0 37.0 5 33.6628340982707 35.0 35.0 37.0 28.0 37.0 6 33.870661467393134 37.0 35.0 37.0 30.0 37.0 7 33.87201709574306 37.0 35.0 37.0 30.0 37.0 8 33.89165197927277 37.0 35.0 37.0 30.0 37.0 9 35.48129322343456 39.0 35.0 39.0 30.0 39.0 10 35.364642724617006 39.0 35.0 39.0 30.0 39.0 11 35.37556315877595 39.0 35.0 39.0 30.0 39.0 12 35.25965480470559 39.0 35.0 39.0 29.0 39.0 13 35.257822022442426 39.0 35.0 39.0 29.0 39.0 14 36.387835387012295 40.0 36.0 41.0 29.0 41.0 15 36.334886639733035 40.0 36.0 41.0 29.0 41.0 16 36.29202756756734 40.0 36.0 41.0 29.0 41.0 17 36.251809883135635 40.0 36.0 41.0 29.0 41.0 18 36.22259869097936 40.0 36.0 41.0 29.0 41.0 19 36.25890499141336 40.0 37.0 41.0 29.0 41.0 20 36.19475931632365 40.0 36.0 41.0 29.0 41.0 21 36.09979929713524 40.0 36.0 41.0 28.0 41.0 22 36.00430844421837 40.0 36.0 41.0 27.0 41.0 23 35.902582212128145 40.0 36.0 41.0 27.0 41.0 24 35.79385630254285 40.0 36.0 41.0 27.0 41.0 25 35.70004315686733 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7676765129984453 1101 2 3.3711702802330024 1101 3 1.8927501549201828 1101 4 1.0883728558167007 1101 5 0.35303439411889315 1101 6 0.3599561266340743 1101 7 0.19991691950988155 1101 8 0.16379160398526693 1101 9 0.2603541402068572 1101 10 0.26502728303893264 1101 11 0.3016195453752033 1101 12 0.19647270590939314 1101 13 0.12947019353874367 1101 14 0.12081087835095872 1101 15 0.35054806426305163 1101 16 0.2679069478391298 1101 17 0.3327184777848444 1101 18 0.4765312481237274 1101 19 0.4283228299875006 1101 20 0.36702648029604745 1101 21 0.4242364496030149 1101 22 0.484642538543568 1101 23 0.3130637777082228 1101 24 0.31124510329317445 1101 25 0.3120176849435268 1102 1 1.0549899450737286 1102 2 3.8095832724760648 1102 3 2.116623103964745 1102 4 1.1524093207796255 1102 5 0.8171125207463064 1102 6 0.6293026601655001 1102 7 0.6497829968256994 1102 8 0.5813083364226017 1102 9 0.4985345282840399 1102 10 0.686137639774067 1102 11 0.635592411076054 1102 12 0.7486365137039996 1102 13 0.8999427538090501 1102 14 0.935613569400978 1102 15 0.7808323439758809 1102 16 0.7983490078576096 1102 17 0.8022327993896567 1102 18 0.8022333358337619 1102 19 0.9111882475244144 1102 20 0.9793989347612069 1102 21 0.9044415538091499 1102 22 0.9019572812083894 1102 23 0.8534275916583169 1102 24 0.5154645086197931 1102 25 0.578393073254091 1103 1 1.2086820448378575 1103 2 3.793068331426106 1103 3 2.3850540670146074 1103 4 1.4767650310319382 1103 5 1.1623157436421963 1103 6 1.0478786645940872 1103 7 1.0042418807607874 1103 8 0.8697783815204616 1103 9 0.9095389045172055 1103 10 1.0061428001374964 1103 11 1.2708590301825566 1103 12 1.0538994719554324 1103 13 1.121183260133165 1103 14 1.4025636345871177 1103 15 1.2186297143637361 1103 16 1.0488718107568147 1103 17 1.2159783717405404 1103 18 1.1626839311820447 1103 19 1.2177438649613066 1103 20 1.3453629398433407 1103 21 1.3532801658136364 1103 22 1.3070033342909895 1103 23 1.3461324538388837 1103 24 1.411142388145059 1103 25 1.3079728414684197 1104 1 1.1610975893734157 1104 2 3.7233803582736016 1104 3 2.100865412104241 1104 4 1.3386354886728355 1104 5 1.0387664277955295 1104 6 0.8530470584060197 1104 7 0.7237508021688939 1104 8 0.7462281150911636 1104 9 0.8382305682117206 1104 10 0.8752354043764896 1104 11 0.8453666473912946 1104 12 0.8405088805941787 1104 13 0.8701813460399777 1104 14 0.9673591490538485 1104 15 1.0226479031920306 1104 16 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7 -1.4131994421374756 2315 8 -1.338640150559172 2315 9 -1.389835602853168 2315 10 -1.483080832376423 2315 11 -1.4962124131239634 2315 12 -1.4086385021029315 2315 13 -1.3902630500415682 2315 14 -1.6415761475773536 2315 15 -1.6398954575400637 2315 16 -1.52394917880968 2315 17 -1.4935035347671786 2315 18 -1.321838847205349 2315 19 -1.5212697805012567 2315 20 -1.3234110448776661 2315 21 -1.460180944920431 2315 22 -1.3887627219909149 2315 23 -1.6282756682586736 2315 24 -1.5076601548149284 2315 25 -1.3678331896284774 2316 1 -1.3953957670679493 2316 2 -1.25079369956444 2316 3 -0.9217060358857019 2316 4 -1.0244467147457996 2316 5 -1.196388022199372 2316 6 -1.2980974687289262 2316 7 -1.136977780425319 2316 8 -1.2976825791692121 2316 9 -1.4066217782728003 2316 10 -1.499366152017302 2316 11 -1.5396868748841683 2316 12 -1.520542534772666 2316 13 -1.4516452078051287 2316 14 -1.5700459551022021 2316 15 -2.234532577319065 2316 16 -1.9563045221721396 2316 17 -1.7727117142104731 2316 18 -1.747715194681554 2316 19 -1.8752577095166671 2316 20 -1.8775482096976503 2316 21 -1.8329002194602637 2316 22 -1.8528440359866565 2316 23 -1.851659147224538 2316 24 -1.8811466275050748 2316 25 -1.9004551756357415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70331.0 3 3.0 4 666.0 5 3202.0 6 4065.0 7 3597.0 8 3299.0 9 3135.0 10 2947.0 11 3034.0 12 2965.0 13 2848.0 14 2862.0 15 3045.0 16 3087.0 17 3503.0 18 3858.0 19 4916.0 20 6226.0 21 7950.0 22 9771.0 23 11862.0 24 13657.0 25 15982.0 26 18295.0 27 20746.0 28 24375.0 29 30240.0 30 37627.0 31 48810.0 32 65014.0 33 86814.0 34 120765.0 35 177202.0 36 275508.0 37 376202.0 38 878842.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72734360321925 19.22223060081772 13.177414771577475 24.873011024385548 2 15.778986184497072 21.1072760988151 40.74683799157459 22.366899725113246 3 18.68164077893672 24.750910746230378 29.85543514519751 26.712013329635393 4 11.771813069842125 17.033414832925835 39.32161494446056 31.873157152771476 5 12.717138555423107 38.13126428809399 36.25901806125849 12.892579095224422 6 30.963191064868408 37.915601758499065 18.48260225142072 12.638604925211814 7 27.34530734037391 31.822757770685794 22.66050797294367 18.171426915996626 8 24.1482057095726 37.852577334081445 20.98505869206148 17.014158264284475 9 25.70653926657183 15.649753690593805 20.960391538868233 37.68331550396613 10 14.238613595222665 29.78381945518396 35.23555853208711 20.742008417506266 11 33.61067904540248 22.77830534527411 25.196623624827513 18.414391984495907 12 22.622676484108432 25.033752248907337 32.67933425100256 19.664237015981673 13 28.424648780188544 22.497324526424137 26.2505708813044 22.827455812082917 14 20.129249066248136 22.858441640881182 28.614834970780713 28.397474322089966 15 22.27622866067583 31.787184242332838 24.04216677296122 21.894420324030108 16 21.26683511903925 28.99898647396465 28.63937431489006 21.094804092106042 17 20.863918978678733 29.226016664721115 28.557229487984582 21.35283486861557 18 20.477614025939282 28.1835645186646 30.885065125118704 20.453756330277418 19 22.515412776106075 27.516475726182353 28.639198105350065 21.3289133923615 20 22.525888949432886 27.871493164090378 29.41181482739067 20.190803059086065 21 22.185256664238274 27.349556711528056 28.26650988612109 22.19867673811258 22 22.068484816466622 28.63888429702771 28.611148508743376 20.68148237776229 23 21.389942605127366 28.264418587213626 28.88740346141524 21.45823534624377 24 21.997113165940128 27.327275639823995 29.74747018585485 20.928141008381022 25 21.80116835489632 27.97554736813404 29.184758898992513 21.038525377977123 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 224.0 1 224.0 2 457.5 3 691.0 4 691.0 5 691.0 6 2020.5 7 3350.0 8 3350.0 9 3350.0 10 5030.5 11 6711.0 12 6711.0 13 6711.0 14 10352.0 15 13993.0 16 13993.0 17 13993.0 18 24202.0 19 34411.0 20 34411.0 21 34411.0 22 53040.5 23 71670.0 24 71670.0 25 71670.0 26 99966.5 27 128263.0 28 128263.0 29 128263.0 30 163182.0 31 198101.0 32 198101.0 33 198101.0 34 220593.0 35 243085.0 36 243085.0 37 243085.0 38 258799.0 39 274513.0 40 274513.0 41 274513.0 42 285364.5 43 296216.0 44 296216.0 45 296216.0 46 298267.0 47 300318.0 48 300318.0 49 300318.0 50 286229.5 51 272141.0 52 272141.0 53 272141.0 54 238261.0 55 204381.0 56 204381.0 57 204381.0 58 172564.5 59 140748.0 60 140748.0 61 140748.0 62 112957.0 63 85166.0 64 85166.0 65 85166.0 66 64481.0 67 43796.0 68 43796.0 69 43796.0 70 31565.0 71 19334.0 72 19334.0 73 19334.0 74 13083.0 75 6832.0 76 6832.0 77 6832.0 78 4602.5 79 2373.0 80 2373.0 81 2373.0 82 1507.0 83 641.0 84 641.0 85 641.0 86 420.5 87 200.0 88 200.0 89 200.0 90 131.5 91 63.0 92 63.0 93 63.0 94 40.5 95 18.0 96 18.0 97 18.0 98 15.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.413311145676368 3 0.0 4 0.0 5 7.24251475449366E-4 6 9.37266615287415E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.2780908390282931E-4 14 0.0 15 0.0 16 0.0 17 7.24251475449366E-4 18 0.0 19 4.260302796760977E-4 20 5.964423915465368E-4 21 8.946635873198052E-4 22 0.004388111880663807 23 1.2780908390282931E-4 24 0.0018319302026072201 25 8.094575313845856E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2347251.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.1183124206806 #Duplication Level Percentage of deduplicated Percentage of total 1 84.35394983574035 62.521724078438226 2 11.155835170319484 16.53703352934712 3 2.5742112796952963 5.72388587595888 4 0.8528368812001598 2.5284332161868925 5 0.3624532067244726 1.3432210006940999 6 0.193277680367692 0.8595249298462224 7 0.11106509226515035 0.5762370045278091 8 0.0652221266586922 0.38673231683441195 9 0.05097660173807333 0.3400469724390416 >10 0.24707028139391282 3.2385755503202454 >50 0.01685455874150046 0.8652416903895541 >100 0.013176013684003795 1.8949534942819497 >500 0.0016225585131241153 0.8004677948823331 >1k 0.0013907644398174455 2.0688808934708787 >5k 5.79485183257269E-5 0.3150416523824222 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7335 0.31249321014241765 No Hit GGTATCAACGCAGAGTACTTTTTTT 4647 0.19797627096548262 No Hit TATCAACGCAGAGTACTTTTTTTTT 4616 0.19665557709848672 No Hit GTCCTACAGTGGACATTTCTAAATT 4089 0.17420378135955633 No Hit CTGTAGGACGTGGAATATGGCAAGA 3930 0.1674298999127064 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3213 0.1368835288599302 No Hit CTTTAGGACGTGAAATATGGCGAGG 2998 0.1277238778468941 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.260302796760977E-5 0.0 3 0.0 0.0 0.0 4.260302796760977E-5 0.0 4 0.0 0.0 0.0 4.260302796760977E-5 0.0 5 0.0 0.0 0.0 4.260302796760977E-5 0.0 6 0.0 0.0 0.0 4.260302796760977E-5 0.0 7 0.0 0.0 0.0 4.260302796760977E-5 0.0 8 4.260302796760977E-5 0.0 0.0 4.260302796760977E-5 0.0 9 8.520605593521953E-5 0.0 0.0 1.2780908390282931E-4 0.0 10 8.520605593521953E-5 0.0 0.0 1.7041211187043907E-4 0.0 11 8.520605593521953E-5 0.0 0.0 1.7041211187043907E-4 0.0 12 8.520605593521953E-5 0.0 0.0 1.7041211187043907E-4 8.520605593521953E-5 13 8.520605593521953E-5 0.0 0.0 2.1301513983804885E-4 1.2780908390282931E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGCG 70 4.7353933E-7 14.843514 7 GGTATCA 1360 0.0 13.878171 1 ATCGCGC 70 7.707951E-6 13.494104 8 GTATCGC 85 2.895522E-7 13.33535 6 CGGTAGG 60 2.6844072E-4 13.315212 1 GCGCGCC 80 2.133158E-6 12.988075 11 CGCGCCT 80 2.133158E-6 12.988075 12 TGTATCG 125 1.1277734E-10 12.846387 5 TCGCGCG 75 1.5688274E-5 12.594497 9 ATACCGC 55 0.0032034072 12.02202 6 GTCCTAA 1065 0.0 11.158524 1 GGCGAGG 960 0.0 10.92179 19 TTAGGAC 1260 0.0 10.870252 3 TAGGACC 715 0.0 10.833029 4 AGGACGT 2300 0.0 10.719012 5 GGACGTG 2285 0.0 10.706701 6 GTCCTAC 2250 0.0 10.696553 1 TCCAACG 240 0.0 10.626607 18 GTATCAA 2840 0.0 10.478697 1 TCCTACA 2450 0.0 10.230974 2 >>END_MODULE