FastQCFastQC Report
Thu 26 May 2016
SRR1511862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347251
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94530.4027264233778151No Hit
TATCAACGCAGAGTACTTTTTTTTT64810.2761102242580789No Hit
GGTATCAACGCAGAGTACTTTTTTT64790.2760250182021437No Hit
CTGTAGGACGTGGAATATGGCAAGA43220.1841302868760094No Hit
GTCCTACAGTGGACATTTCTAAATT41360.17620612367403402No Hit
GTCCTAAAGTGTGTATTTCTCATTT32690.13926929842611632No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32510.13850244392269936No Hit
CTTTAGGACGTGAAATATGGCGAGG31950.13611667435651323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC456.777903E-414.7738196
CGTGCGC551.9652848E-413.8144810
GGTATCA17400.013.4794051
TAGGACC8350.012.9701774
CCAACGA1850.012.83521719
TCCAACG2600.012.785851518
TCCCGTT1603.6379788E-1211.87181911
GCGTGCG658.0441614E-411.6891769
GTGGTAT5550.010.994411
GCCGGTT1605.475158E-1010.68463711
CCGGTTT1701.4006218E-1010.61480112
GACGTGA11100.010.609777
TTAGGAC14100.010.578113
CCCGTTT1909.094947E-1210.49718712
AGGACGT21400.010.4767295
TGGTATC5300.010.396322
GGACGTG21200.010.3934416
GGCGAGG11450.010.36906319
CCATTCG750.002654326410.1306189
TGTAGGA23600.010.1039052
Produced by FastQC (version 0.11.5)