##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511862_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2347251 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.56097920503602 31.0 31.0 34.0 27.0 34.0 2 30.75853796632742 31.0 31.0 34.0 27.0 34.0 3 30.861808984211745 31.0 31.0 34.0 27.0 34.0 4 34.45590906128062 37.0 35.0 37.0 31.0 37.0 5 34.059588855218294 37.0 35.0 37.0 30.0 37.0 6 33.996964108227026 37.0 35.0 37.0 30.0 37.0 7 33.8191688916098 37.0 35.0 37.0 29.0 37.0 8 33.72460039424842 37.0 35.0 37.0 29.0 37.0 9 35.1725972211749 39.0 35.0 39.0 29.0 39.0 10 34.96738141766688 38.0 35.0 39.0 28.0 39.0 11 34.951008647988644 38.0 35.0 39.0 28.0 39.0 12 34.7495372246087 38.0 35.0 39.0 27.0 39.0 13 34.740856005599746 38.0 35.0 39.0 27.0 39.0 14 35.68781054944699 39.0 36.0 41.0 27.0 41.0 15 35.61805490763451 39.0 36.0 41.0 27.0 41.0 16 35.50441047847035 39.0 35.0 41.0 27.0 41.0 17 35.44109258021405 39.0 35.0 41.0 27.0 41.0 18 35.36250447864332 39.0 35.0 40.0 27.0 41.0 19 35.38376807593223 39.0 35.0 40.0 27.0 41.0 20 35.237963899046164 39.0 35.0 40.0 26.0 41.0 21 35.11759458191732 39.0 35.0 40.0 26.0 41.0 22 35.20754874531953 39.0 35.0 40.0 26.0 41.0 23 35.09227815857784 39.0 35.0 40.0 26.0 41.0 24 34.94409460257978 39.0 35.0 41.0 25.0 41.0 25 34.47169433520318 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3488955693662348 1101 2 0.21662044900259403 1101 3 0.3218598864794977 1101 4 0.32616631657251105 1101 5 0.38433838197084214 1101 6 0.3660457715290022 1101 7 0.3084306266837018 1101 8 0.347685184437168 1101 9 0.3527967212149292 1101 10 0.4311135155433803 1101 11 0.14525732043048833 1101 12 0.3759451063999819 1101 13 0.3124965847926475 1101 14 0.26039998412215226 1101 15 0.09082670787173441 1101 16 0.31826854028068396 1101 17 0.53725109159884 1101 18 0.4554989753754981 1101 19 0.31897161066042656 1101 20 0.33151099686953245 1101 21 0.3759253350008649 1101 22 0.33996960277658417 1101 23 0.2137624515808838 1101 24 0.03841780133545569 1101 25 0.35697882622049804 1102 1 0.4221878946313211 1102 2 0.3372281141373854 1102 3 0.47823828934983226 1102 4 0.4132055643471091 1102 5 0.5772119137487621 1102 6 0.5881582910426175 1102 7 0.5344846983878284 1102 8 0.5992735353955254 1102 9 0.5947083149183143 1102 10 0.6323360938011717 1102 11 0.646588801953115 1102 12 0.7524185769491183 1102 13 0.8280107649434711 1102 14 0.9070020616205952 1102 15 0.9449531999412457 1102 16 1.1222098478821678 1102 17 1.166092948282305 1102 18 1.0041792239350187 1102 19 1.108678448289389 1102 20 1.153517853922942 1102 21 1.1114159571517845 1102 22 0.814054885671311 1102 23 0.9754330680206493 1102 24 1.0251967452367197 1102 25 1.0384332096533413 1103 1 0.6132936170831904 1103 2 0.5809203502204561 1103 3 0.6217044928218556 1103 4 0.6326600949664183 1103 5 0.778445738469081 1103 6 0.8538267210968726 1103 7 0.8589873960211563 1103 8 0.7798352840237897 1103 9 0.8946277682042805 1103 10 1.017045731491109 1103 11 0.989926233415801 1103 12 1.1074914888145742 1103 13 0.9315908362204937 1103 14 1.2738278394930305 1103 15 1.1876179073030002 1103 16 0.9905208107502688 1103 17 1.063360622774013 1103 18 1.1853994523225921 1103 19 1.2918296641259346 1103 20 1.2495820099634827 1103 21 1.0596582231756315 1103 22 1.3714906987120585 1103 23 1.387849431904506 1103 24 1.5578898800369885 1103 25 1.4793173150425076 1104 1 0.4127836104899494 1104 2 0.4904178494276721 1104 3 0.5518687991284743 1104 4 0.4358248507085065 1104 5 0.5726269179976313 1104 6 0.5442620727326002 1104 7 0.6065438283096327 1104 8 0.7579608567498255 1104 9 0.8751566039382794 1104 10 0.7443941078579499 1104 11 0.9135727748539821 1104 12 0.8849215012177751 1104 13 1.0353714554376268 1104 14 1.1554829635516697 1104 15 1.1661617363867194 1104 16 1.0816210379014919 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0.849975839973176 1107 17 1.002517646323561 1107 18 1.0518763770046675 1107 19 1.1231165881063845 1107 20 0.9781152515885907 1107 21 0.9166791916159127 1107 22 0.8612827665567551 1107 23 1.007895288439066 1107 24 0.7591229605789351 1107 25 0.8684945694161286 1108 1 0.1831241946931037 1108 2 0.15058906908500447 1108 3 0.16533508925507334 1108 4 0.29340651307393273 1108 5 0.2945162705851203 1108 6 0.2631440041605444 1108 7 0.4029324970031283 1108 8 0.3343429326624161 1108 9 0.37704685554134443 1108 10 0.2552309914703912 1108 11 0.5535387977731645 1108 12 0.4134251111934262 1108 13 0.5067651008245022 1108 14 0.6885153645011997 1108 15 0.7558221296846312 1108 16 0.6659356776566767 1108 17 0.5293160021476027 1108 18 0.4883020167901506 1108 19 0.6116350867913241 1108 20 0.513375635727634 1108 21 0.5119013126110801 1108 22 0.6642303043005953 1108 23 0.559429276227462 1108 24 0.6565023206518958 1108 25 0.556108812255772 1109 1 0.06689517312104343 1109 2 -0.013478099463469562 1109 3 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-0.6727130132139649 2314 8 -0.6412799360618351 2314 9 -0.7437271380819794 2314 10 -0.7714024383640563 2314 11 -0.7979529500150448 2314 12 -0.9145789925378978 2314 13 -0.7221703815978842 2314 14 -0.7660865581729226 2314 15 -0.7483174009487428 2314 16 -0.8745341851346211 2314 17 -0.7593845425985464 2314 18 -0.8237859967532941 2314 19 -0.7564873242356143 2314 20 -0.8710158341134857 2314 21 -0.8642955697715493 2314 22 -0.9219923316025316 2314 23 -1.0200320006962045 2314 24 -1.1982437099426946 2314 25 -0.9405133345157424 2315 1 -0.7911233009519769 2315 2 -0.8256377637800014 2315 3 -0.9124904705799217 2315 4 -0.9660551538564803 2315 5 -0.8859537959980273 2315 6 -0.9859610542297474 2315 7 -1.0066333223428714 2315 8 -1.114048347535956 2315 9 -1.1960127225404946 2315 10 -1.2954407033453776 2315 11 -1.2267992184259384 2315 12 -1.222527520893209 2315 13 -1.3628585508525006 2315 14 -1.4687476903295007 2315 15 -1.4852637601218603 2315 16 -1.44238362263075 2315 17 -1.5908091824991146 2315 18 -1.4333349540389762 2315 19 -1.4636862757391498 2315 20 -1.540971025374148 2315 21 -1.5863499733749364 2315 22 -1.4494575423356082 2315 23 -1.3530067239414976 2315 24 -1.4023261412022805 2315 25 -1.393129494397435 2316 1 -1.7251322490947878 2316 2 -1.3240481172016167 2316 3 -1.3347118920734822 2316 4 -0.9864764372243471 2316 5 -1.0921030918572612 2316 6 -1.0932561928058533 2316 7 -1.200179249136248 2316 8 -1.3153473296096436 2316 9 -1.62085012457635 2316 10 -1.6961370370090094 2316 11 -1.6468370528552683 2316 12 -1.7137175037776373 2316 13 -1.7493225330763238 2316 14 -1.9902145251491845 2316 15 -1.8959676006167072 2316 16 -1.976182679772748 2316 17 -2.0461705613538754 2316 18 -2.0997944321640674 2316 19 -1.9808270738654485 2316 20 -1.9822492885936782 2316 21 -2.1127049564094946 2316 22 -1.9865106606732965 2316 23 -1.968602916476705 2316 24 -1.844853841409467 2316 25 -2.0218782557702895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44537.0 3 0.0 4 791.0 5 6863.0 6 8607.0 7 6911.0 8 5679.0 9 4944.0 10 4656.0 11 4476.0 12 4400.0 13 4159.0 14 4044.0 15 4122.0 16 4122.0 17 4345.0 18 4705.0 19 5303.0 20 6349.0 21 8523.0 22 10510.0 23 13274.0 24 16442.0 25 19222.0 26 21986.0 27 25166.0 28 29263.0 29 35614.0 30 45232.0 31 57808.0 32 73968.0 33 96313.0 34 126556.0 35 168542.0 36 251773.0 37 443912.0 38 774134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.453405050877855 20.431246142943838 14.06224406574669 24.053104740431614 2 14.935002690381216 21.37270364353876 41.035406950513604 22.656886715566422 3 18.952127403503077 25.5228350099755 30.381241716373747 25.143795870147674 4 12.60214608493084 18.03952794140891 38.73318192217194 30.625144051488316 5 12.633867560658288 38.490234289271974 36.240283402276205 12.635614747793536 6 30.943686891602134 38.49094110514811 18.106116474122285 12.459255529127478 7 27.27829277738086 32.08825984097994 22.629833792806988 18.003613588832213 8 24.325008275638183 38.2032854603108 20.622975557364764 16.848730706686247 9 25.740621688945918 15.478276503024176 20.842594166537793 37.938507641492116 10 14.242746088935526 30.17772705177248 35.058372538769824 20.52115432052218 11 33.84959682624483 22.823336745835874 24.959537774187762 18.367528653731537 12 22.57619658059577 25.43528578750206 32.56383744218236 19.424680189719805 13 28.634858393925487 22.486815427919723 26.29693202814697 22.581394150007817 14 20.119620781927456 23.02962060725504 28.629788633597343 28.22096997722016 15 22.350826562647114 31.966202165852735 24.010129295929577 21.672841975570574 16 21.279156459686867 29.254531899030784 28.53172861859623 20.93458302268612 17 20.842594166537793 29.450152540141637 28.585992720846644 21.121260572473926 18 20.57740031398512 28.27847114383032 30.829333963859757 20.3147945783248 19 22.598563170278766 27.548012547443797 28.779154849651782 21.074269432625655 20 22.691821198499863 27.864638251299073 29.433281741066462 20.0102588091346 21 22.186677095887912 27.366140221050074 28.427424250751198 22.01975843231082 22 22.063682154145422 28.671028364669993 28.748736287682913 20.516553193501675 23 21.443254257853123 28.307496727022375 29.0223329332909 21.2269160818336 24 22.090867260859934 27.41210654578299 29.759539187784384 20.737487005572692 25 21.83441126186442 27.985995520713825 29.330335213690578 20.849258003731176 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 220.0 1 220.0 2 502.5 3 785.0 4 785.0 5 785.0 6 2224.0 7 3663.0 8 3663.0 9 3663.0 10 5415.0 11 7167.0 12 7167.0 13 7167.0 14 10960.5 15 14754.0 16 14754.0 17 14754.0 18 25208.0 19 35662.0 20 35662.0 21 35662.0 22 54760.0 23 73858.0 24 73858.0 25 73858.0 26 103105.5 27 132353.0 28 132353.0 29 132353.0 30 167046.5 31 201740.0 32 201740.0 33 201740.0 34 223722.0 35 245704.0 36 245704.0 37 245704.0 38 260840.0 39 275976.0 40 275976.0 41 275976.0 42 285382.0 43 294788.0 44 294788.0 45 294788.0 46 296530.0 47 298272.0 48 298272.0 49 298272.0 50 283495.0 51 268718.0 52 268718.0 53 268718.0 54 235088.5 55 201459.0 56 201459.0 57 201459.0 58 169855.0 59 138251.0 60 138251.0 61 138251.0 62 110524.5 63 82798.0 64 82798.0 65 82798.0 66 62790.5 67 42783.0 68 42783.0 69 42783.0 70 30762.5 71 18742.0 72 18742.0 73 18742.0 74 12632.0 75 6522.0 76 6522.0 77 6522.0 78 4352.5 79 2183.0 80 2183.0 81 2183.0 82 1402.0 83 621.0 84 621.0 85 621.0 86 400.5 87 180.0 88 180.0 89 180.0 90 111.0 91 42.0 92 42.0 93 42.0 94 26.0 95 10.0 96 10.0 97 10.0 98 5.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38359766382035837 2 0.0 3 0.0 4 0.0 5 0.026499083395853277 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 4.260302796760977E-5 17 0.0 18 2.5561816780565863E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.003876875545052489 25 8.520605593521953E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2347251.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.57300211667497 #Duplication Level Percentage of deduplicated Percentage of total 1 83.62376671832652 60.688277990534445 2 11.597387705917187 16.833144850588564 3 2.7358216451424244 5.956403701312992 4 0.8976714536069602 2.6058684921078648 5 0.38743689464991965 1.4058729287753298 6 0.19917334063550557 0.8672764362915463 7 0.1230204221004665 0.6249572947443983 8 0.08108465186658992 0.4707645289235106 9 0.051536330175184726 0.3366131579000407 >10 0.26633959956222397 3.39119676522198 >50 0.01917063890974224 0.9707480156349414 >100 0.014311253387582903 2.0902602530810386 >500 0.0017567923498310093 0.9326310083599828 >1k 0.0013468741348673411 1.8734642397569363 >5k 1.7567923498269664E-4 0.9525203367663774 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9453 0.4027264233778151 No Hit TATCAACGCAGAGTACTTTTTTTTT 6481 0.2761102242580789 No Hit GGTATCAACGCAGAGTACTTTTTTT 6479 0.2760250182021437 No Hit CTGTAGGACGTGGAATATGGCAAGA 4322 0.1841302868760094 No Hit GTCCTACAGTGGACATTTCTAAATT 4136 0.17620612367403402 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3269 0.13926929842611632 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3251 0.13850244392269936 No Hit CTTTAGGACGTGAAATATGGCGAGG 3195 0.13611667435651323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.520605593521953E-5 0.0 10 0.0 0.0 0.0 8.520605593521953E-5 0.0 11 0.0 0.0 0.0 8.520605593521953E-5 0.0 12 0.0 0.0 0.0 8.520605593521953E-5 4.260302796760977E-5 13 0.0 0.0 0.0 1.7041211187043907E-4 4.260302796760977E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGC 45 6.777903E-4 14.773819 6 CGTGCGC 55 1.9652848E-4 13.81448 10 GGTATCA 1740 0.0 13.479405 1 TAGGACC 835 0.0 12.970177 4 CCAACGA 185 0.0 12.835217 19 TCCAACG 260 0.0 12.7858515 18 TCCCGTT 160 3.6379788E-12 11.871819 11 GCGTGCG 65 8.0441614E-4 11.689176 9 GTGGTAT 555 0.0 10.99441 1 GCCGGTT 160 5.475158E-10 10.684637 11 CCGGTTT 170 1.4006218E-10 10.614801 12 GACGTGA 1110 0.0 10.60977 7 TTAGGAC 1410 0.0 10.57811 3 CCCGTTT 190 9.094947E-12 10.497187 12 AGGACGT 2140 0.0 10.476729 5 TGGTATC 530 0.0 10.39632 2 GGACGTG 2120 0.0 10.393441 6 GGCGAGG 1145 0.0 10.369063 19 CCATTCG 75 0.0026543264 10.130618 9 TGTAGGA 2360 0.0 10.103905 2 >>END_MODULE