FastQCFastQC Report
Thu 26 May 2016
SRR1511861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372167
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80550.3395629397087136No Hit
GGTATCAACGCAGAGTACTTTTTTT50860.21440311748709093No Hit
TATCAACGCAGAGTACTTTTTTTTT50670.21360216207374944No Hit
GTCCTACAGTGGACATTTCTAAATT34120.14383473001690017No Hit
CTGTAGGACGTGGAATATGGCAAGA30780.12975477696131849No Hit
GTCCTAAAGTGTGTATTTCTCATTT28320.11938451213594994No Hit
CTTTAGGACGTGAAATATGGCGAGG25710.10838191408952236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.012.8645791
GTGGTAT4800.012.4928221
TGGTATC4400.012.2656792
CTTACAC3000.011.0192373
TAGGACC6200.010.8161154
GTATCAA30200.010.7554081
GGACGTG18850.010.1716036
AGGACGT19350.010.1528295
GTCTTAG3950.010.1207661
GGACGAT750.00277895110.0747316
GACGTGA10550.010.0267727
AGGACCT13500.010.00476555
AGCCCGT856.956785E-410.000656
TGTCCGT856.958133E-410.0004410
GTCCTAC17400.09.9942571
GTCCTAA10050.09.9445341
TTAGGAC12700.09.8169123
GGCGAGG9550.09.79162719
TGTAGGA20050.09.72012
TAGGACG20900.09.7162094
Produced by FastQC (version 0.11.5)