FastQCFastQC Report
Thu 26 May 2016
SRR1511861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372167
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105750.4457949208466352No Hit
TATCAACGCAGAGTACTTTTTTTTT70490.2971544583496861No Hit
GGTATCAACGCAGAGTACTTTTTTT70260.2961848807440623No Hit
GTCCTACAGTGGACATTTCTAAATT37770.15922150506267055No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32630.13755355335438021No Hit
CTGTAGGACGTGGAATATGGCAAGA32340.13633104246033267No Hit
GTCCTAAAGTGTGTATTTCTCATTT31130.13123022114378963No Hit
CTTTAGGACGTGAAATATGGCGAGG29500.1243588668082812No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27810.11723457918434915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.013.8763271
ACGTACA802.0020125E-613.0617182
TAGGACC7100.012.3091224
TATACCG550.00307064112.0901855
GGACCGT752.0799164E-411.3969146
TGGACCG855.3286174E-511.17585
TAGGACT3650.010.9308524
GTCCTAC18750.010.6810221
GTGGTAT5750.010.6146781
TATGTCG909.5431256E-510.5529216
TGTAGGA21200.010.4852212
GGCGAGG8350.010.46445419
TCCTACA19800.010.4109932
GTAGGAC20250.010.3672793
TGGCGAG17900.010.24067618
AGGACGG951.645645E-49.9994025
GTATCAA38000.09.8879751
TGTCCAC6550.09.85992610
ATAGGAC2700.09.8512613
GGTCTAT1856.0572347E-109.7943731
Produced by FastQC (version 0.11.5)