FastQCFastQC Report
Thu 26 May 2016
SRR1511860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2672222
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76290.2854927472343241No Hit
GTCCTACAGTGGACATTTCTAAATT51060.1910769389668972No Hit
TATCAACGCAGAGTACTTTTTTTTT47470.17764242641517058No Hit
CTGTAGGACGTGGAATATGGCAAGA46430.17375053419962863No Hit
GGTATCAACGCAGAGTACTTTTTTT44460.1663783922144193No Hit
GTCCTAAAGTGTGTATTTCTCATTT40470.1514469980413304No Hit
CTTTAGGACGTGAAATATGGCGAGG37230.1393222569082958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1700.013.33491919
GGTATCA14000.013.2751941
TAGGACC9200.012.8331714
ACCGCCC604.298366E-412.5936178
GGCGAGG11250.011.41864119
TCCAACG2400.011.41339318
GCGCCAC1002.0701882E-611.33425613
TGTAGGA26800.011.1851672
TATGTCG1803.6379788E-1211.01962316
GCACCGT600.0061360111.0194166
ATGTCGA1900.010.93676917
CGGTAGG1207.965009E-710.8247561
GTCGAGA700.001565252410.79493319
CGTGCTG700.001565740910.7945310
GTAGGAC27800.010.7702623
GGCGTGC1159.505675E-710.6771988
CTGTAGG27950.010.5104541
GGACGTG27550.010.4908726
TGTCGAG1901.0913936E-1110.43983818
GTATCAA28150.010.4002851
Produced by FastQC (version 0.11.5)