FastQCFastQC Report
Thu 26 May 2016
SRR1511860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2672222
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96100.35962580953229184No Hit
TATCAACGCAGAGTACTTTTTTTTT66130.24747195405172176No Hit
GGTATCAACGCAGAGTACTTTTTTT64870.24275677694443049No Hit
GTCCTACAGTGGACATTTCTAAATT53100.1987110352358449No Hit
CTGTAGGACGTGGAATATGGCAAGA48170.1802619692525546No Hit
GTCCTAAAGTGTGTATTTCTCATTT41530.15541373433794048No Hit
CTTTAGGACGTGAAATATGGCGAGG40470.1514469980413304No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31690.11859044645242797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.013.7443291
TAGGACC9600.012.7654254
TGAACCG701.0916274E-412.2140935
AATCCCG2250.010.97555219
GGCGAGG12200.010.89942719
AAGGCGA803.7816298E-410.684731517
CCAACGT1252.2482709E-710.63784119
AGGACGT27550.010.62052255
GTAGGAC28100.010.6154923
TGTAGGA28900.010.4202512
TCCAACG2100.010.40265118
GGACGTG27550.010.3766236
TTAGGAC17250.010.3534593
AGGACCT18100.010.3396165
GACGTGG13800.010.3234137
GCCGGTT1201.5298447E-610.289000511
TAGGACG29400.010.2430354
GGACCTG17150.010.1344016
CTGTAGG28350.010.1226361
TTTAGGA19050.09.9735972
Produced by FastQC (version 0.11.5)