##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511860_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2672222 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.693012032682912 31.0 31.0 34.0 27.0 34.0 2 30.898819035244827 31.0 31.0 34.0 27.0 34.0 3 31.00433534339587 31.0 31.0 34.0 27.0 34.0 4 34.5904831260277 37.0 35.0 37.0 31.0 37.0 5 34.20472962201494 37.0 35.0 37.0 30.0 37.0 6 34.14388175832696 37.0 35.0 37.0 30.0 37.0 7 33.975186193362674 37.0 35.0 37.0 30.0 37.0 8 33.881598534852266 37.0 35.0 37.0 29.0 37.0 9 35.35234684842801 39.0 35.0 39.0 30.0 39.0 10 35.15824246638191 38.0 35.0 39.0 29.0 39.0 11 35.13282504223077 39.0 35.0 39.0 29.0 39.0 12 34.95820407136832 38.0 35.0 39.0 28.0 39.0 13 34.94620918471594 38.0 35.0 39.0 28.0 39.0 14 35.92472855922899 39.0 36.0 41.0 27.0 41.0 15 35.86391512381831 39.0 36.0 41.0 27.0 41.0 16 35.75831424185566 39.0 36.0 41.0 27.0 41.0 17 35.70495527691936 39.0 36.0 41.0 27.0 41.0 18 35.62359788969629 39.0 36.0 41.0 27.0 41.0 19 35.651125542713146 39.0 36.0 41.0 27.0 41.0 20 35.5134184210743 39.0 35.0 41.0 27.0 41.0 21 35.397311675452116 39.0 35.0 41.0 27.0 41.0 22 35.48733750414449 39.0 36.0 41.0 27.0 41.0 23 35.37619030155429 39.0 36.0 41.0 27.0 41.0 24 35.24648812860608 39.0 35.0 41.0 27.0 41.0 25 34.787219774404974 39.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49860611060350024 1101 2 0.4945208221480719 1101 3 0.4831370761011975 1101 4 0.39710628779234725 1101 5 0.5774040716148363 1101 6 0.5521266951463346 1101 7 0.528735587951914 1101 8 0.5619821402945888 1101 9 0.5640943614236207 1101 10 0.6183465799904866 1101 11 0.4620366193123715 1101 12 0.648864818308482 1101 13 0.688840766395451 1101 14 0.6467566211341307 1101 15 0.37486199151867794 1101 16 0.4369222521409881 1101 17 0.45067512929522024 1101 18 0.542470240829175 1101 19 0.5343316624615397 1101 20 0.6398601275492339 1101 21 0.7082018440627991 1101 22 0.5557033741921344 1101 23 0.32286448975433046 1101 24 0.2773691759824928 1101 25 0.36440311238422396 1102 1 0.5308212744228165 1102 2 0.497273709427521 1102 3 0.5814744045326989 1102 4 0.4388999480298139 1102 5 0.4721115679935082 1102 6 0.5487790214308674 1102 7 0.7035770608448715 1102 8 0.5714602882409352 1102 9 0.6729806273004613 1102 10 0.7724931371074604 1102 11 0.794113712678012 1102 12 0.7865298780662613 1102 13 0.8842757631076026 1102 14 1.0985821372287532 1102 15 1.0490764168984583 1102 16 1.0775919399285385 1102 17 1.1020916688451265 1102 18 1.1086326665700028 1102 19 1.1716460243104763 1102 20 1.1759616642026032 1102 21 1.0459605392057014 1102 22 0.7896987929452592 1102 23 1.0692571187155977 1102 24 1.1772831406462672 1102 25 1.2710487725389044 1103 1 0.3592884301233994 1103 2 0.3784924504393459 1103 3 0.4696332987419076 1103 4 0.5141614801161722 1103 5 0.5157641833474713 1103 6 0.685596702115312 1103 7 0.6890917230914084 1103 8 0.6409142805377073 1103 9 0.7566569937760335 1103 10 0.7835601138802772 1103 11 0.8704360355218199 1103 12 0.8739918277831222 1103 13 0.6983495934823623 1103 14 0.930229844811933 1103 15 1.0002946051737567 1103 16 0.7298268324312147 1103 17 0.9260265505247531 1103 18 1.0848105497612153 1103 19 1.0656450598348854 1103 20 0.9547442603497984 1103 21 0.9163458098033317 1103 22 1.0852053097750982 1103 23 1.0452173825860527 1103 24 1.1563382325908407 1103 25 1.0252105972446373 1104 1 0.5723237017810732 1104 2 0.49580659505666347 1104 3 0.5977227699224983 1104 4 0.5571805961620768 1104 5 0.6524604607022937 1104 6 0.5976666672431818 1104 7 0.5992248236265354 1104 8 0.5311556055834714 1104 9 0.7752922745730046 1104 10 0.7037188330077839 1104 11 0.7385686708413317 1104 12 0.7660695803537863 1104 13 0.786481137163598 1104 14 0.9642454837995444 1104 15 0.8974730693085249 1104 16 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-0.25959092125957994 2314 2 -0.35146039498741644 2314 3 -0.5196908914042702 2314 4 -0.4219606906998905 2314 5 -0.5196673898000057 2314 6 -0.5285219136294472 2314 7 -0.5532002383021108 2314 8 -0.5576355212146069 2314 9 -0.6131288284259497 2314 10 -0.7301743081311827 2314 11 -0.7053653415385099 2314 12 -0.7657460438569927 2314 13 -0.7349811899306857 2314 14 -0.8868408550016298 2314 15 -0.853820836953993 2314 16 -0.9367415416999805 2314 17 -0.7305175356156894 2314 18 -0.8059087396793743 2314 19 -0.7976387240676388 2314 20 -0.7928141479003656 2314 21 -0.6964170925779669 2314 22 -0.6822675452035298 2314 23 -0.775104724034037 2314 24 -0.9602107845441026 2314 25 -0.9069486873166426 2315 1 -0.6710109443396526 2315 2 -0.7455937349904218 2315 3 -0.7971498324311774 2315 4 -0.9215865466952451 2315 5 -0.9541507768836581 2315 6 -0.9993535445221369 2315 7 -0.9647025730631995 2315 8 -0.8800467880949085 2315 9 -0.9577673243383487 2315 10 -0.9809384331949147 2315 11 -0.9785012279758618 2315 12 -1.0226023608736128 2315 13 -1.103636040753834 2315 14 -1.2455622337540646 2315 15 -1.3591286590648153 2315 16 -1.2351116474094965 2315 17 -1.2751218562921522 2315 18 -1.4123588593132013 2315 19 -1.309426925349321 2315 20 -1.3472978178983794 2315 21 -1.374034732165967 2315 22 -1.5256757736244921 2315 23 -1.362097100311793 2315 24 -1.286146590453363 2315 25 -1.2813819616548159 2316 1 -1.809707451737065 2316 2 -1.3545621171951865 2316 3 -1.2690258830896894 2316 4 -1.138868088726504 2316 5 -1.2080644717462619 2316 6 -1.3054312915683823 2316 7 -1.3481713663360892 2316 8 -1.3009230392365438 2316 9 -1.7201256068558592 2316 10 -1.6201230936972024 2316 11 -1.4592823558307373 2316 12 -1.6054496046450382 2316 13 -1.5630430769345622 2316 14 -1.8027846254918742 2316 15 -1.7511366866786489 2316 16 -1.8507758552654323 2316 17 -1.7314090227299346 2316 18 -1.7550340502254613 2316 19 -1.8337997162143367 2316 20 -1.9719570354339737 2316 21 -2.150212363325714 2316 22 -1.90378529543338 2316 23 -1.8634296655581721 2316 24 -1.8790076297433984 2316 25 -1.8943012602021625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47116.0 3 1.0 4 874.0 5 7413.0 6 9339.0 7 7460.0 8 6021.0 9 5312.0 10 5026.0 11 4737.0 12 4585.0 13 4482.0 14 4297.0 15 4258.0 16 4470.0 17 4725.0 18 5024.0 19 5693.0 20 6800.0 21 8858.0 22 11376.0 23 14307.0 24 17591.0 25 20673.0 26 24254.0 27 27465.0 28 31730.0 29 38834.0 30 49123.0 31 63262.0 32 81451.0 33 105202.0 34 138166.0 35 185250.0 36 273543.0 37 488981.0 38 954523.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.99953264427166 20.622973163863033 14.261187233825135 24.11630695804017 2 14.490861911921987 21.409186811574788 41.432672884213964 22.667278392289266 3 18.447269725344675 25.757740187753864 30.646967205569 25.148022881332466 4 12.349497908482155 18.204700058602917 39.17773298775326 30.268069045161667 5 12.537782613952364 38.44904779390709 36.43851676172445 12.574652830416094 6 30.420414172175814 38.76152505293348 18.39364394125937 12.424416833631339 7 26.982002243825548 32.19706296857073 23.014480084364248 17.806454703239478 8 24.05354046183289 38.16359568928031 21.058355181567997 16.72450866731881 9 25.603561380753547 15.717593822668924 21.244155612819593 37.43468918375793 10 14.172886833504103 30.17761997319085 35.26802788091708 20.381465312387967 11 33.275004846154246 23.07888341612336 25.405187143882507 18.240924593839882 12 22.525710812948923 25.56292104473356 32.71453494507567 19.196833197241848 13 28.237736235986382 22.77467964862201 26.520326529756883 22.467257585634727 14 19.94707026586863 23.284442684776938 28.846592835475494 27.92189421387894 15 22.038363579073895 32.34686339682856 24.080035266531 21.53473775756655 16 20.952712761140354 29.44905026603329 28.823727968709186 20.774509004117174 17 20.607232482929934 29.69599831151753 28.826384933587107 20.870384271965428 18 20.305925410332982 28.394031928508877 31.212325332495077 20.08771732866306 19 22.440500826652876 27.727748667588248 28.856322566014352 20.975427939744527 20 22.416513298670544 28.228268459731265 29.537740502099002 19.81747773949919 21 21.952143197683426 27.509316216990953 28.58523730438564 21.95330328093998 22 21.919960242824136 28.973603241048085 28.905120906870764 20.201315609257016 23 21.15872109428034 28.60424770097694 29.144958764653534 21.092072440089186 24 21.8739076967755 27.6548160888827 30.03919006207089 20.432086152270905 25 21.593065538415825 28.223884344808564 29.464875446211558 20.718174670564053 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 189.0 1 189.0 2 484.0 3 779.0 4 779.0 5 779.0 6 2248.5 7 3718.0 8 3718.0 9 3718.0 10 5558.5 11 7399.0 12 7399.0 13 7399.0 14 11841.5 15 16284.0 16 16284.0 17 16284.0 18 28519.5 19 40755.0 20 40755.0 21 40755.0 22 63567.5 23 86380.0 24 86380.0 25 86380.0 26 121195.0 27 156010.0 28 156010.0 29 156010.0 30 197501.0 31 238992.0 32 238992.0 33 238992.0 34 266460.5 35 293929.0 36 293929.0 37 293929.0 38 309657.0 39 325385.0 40 325385.0 41 325385.0 42 334145.5 43 342906.0 44 342906.0 45 342906.0 46 341984.0 47 341062.0 48 341062.0 49 341062.0 50 320645.5 51 300229.0 52 300229.0 53 300229.0 54 259154.0 55 218079.0 56 218079.0 57 218079.0 58 181528.0 59 144977.0 60 144977.0 61 144977.0 62 114874.0 63 84771.0 64 84771.0 65 84771.0 66 63647.5 67 42524.0 68 42524.0 69 42524.0 70 30320.0 71 18116.0 72 18116.0 73 18116.0 74 12396.5 75 6677.0 76 6677.0 77 6677.0 78 4450.5 79 2224.0 80 2224.0 81 2224.0 82 1424.5 83 625.0 84 625.0 85 625.0 86 396.5 87 168.0 88 168.0 89 168.0 90 104.5 91 41.0 92 41.0 93 41.0 94 22.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3906112590944914 2 0.0 3 0.0 4 0.0 5 0.025783785927965566 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 7.484408106811484E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004340956701950662 25 1.1226612160217227E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2672222.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.72102204085112 #Duplication Level Percentage of deduplicated Percentage of total 1 85.08750267910641 62.72737660407381 2 10.770326079521514 15.879988925911185 3 2.3286244260924396 5.150057179224751 4 0.7691815367812469 2.2681939610586417 5 0.32861428727369935 1.2112890557521485 6 0.17756412140567163 0.7854125104687129 7 0.10223719263188491 0.5275921231986951 8 0.07927892706582652 0.46756188236758606 9 0.04564441046015167 0.3028457330617047 >10 0.2725719867965589 3.58155999096659 >50 0.020993058979888242 1.0784980963415942 >100 0.014322681078210624 2.1624529475343377 >500 0.0015186836160224167 0.8266299398285927 >1k 0.0014174380415753714 1.984844846444226 >5k 2.0249114879648163E-4 1.0456962037673632 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9610 0.35962580953229184 No Hit TATCAACGCAGAGTACTTTTTTTTT 6613 0.24747195405172176 No Hit GGTATCAACGCAGAGTACTTTTTTT 6487 0.24275677694443049 No Hit GTCCTACAGTGGACATTTCTAAATT 5310 0.1987110352358449 No Hit CTGTAGGACGTGGAATATGGCAAGA 4817 0.1802619692525546 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4153 0.15541373433794048 No Hit CTTTAGGACGTGAAATATGGCGAGG 4047 0.1514469980413304 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3169 0.11859044645242797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 3.742204053405742E-5 0.0 12 0.0 0.0 0.0 3.742204053405742E-5 0.0 13 0.0 0.0 0.0 3.742204053405742E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1755 0.0 13.744329 1 TAGGACC 960 0.0 12.765425 4 TGAACCG 70 1.0916274E-4 12.214093 5 AATCCCG 225 0.0 10.975552 19 GGCGAGG 1220 0.0 10.899427 19 AAGGCGA 80 3.7816298E-4 10.6847315 17 CCAACGT 125 2.2482709E-7 10.637841 19 AGGACGT 2755 0.0 10.6205225 5 GTAGGAC 2810 0.0 10.615492 3 TGTAGGA 2890 0.0 10.420251 2 TCCAACG 210 0.0 10.402651 18 GGACGTG 2755 0.0 10.376623 6 TTAGGAC 1725 0.0 10.353459 3 AGGACCT 1810 0.0 10.339616 5 GACGTGG 1380 0.0 10.323413 7 GCCGGTT 120 1.5298447E-6 10.2890005 11 TAGGACG 2940 0.0 10.243035 4 GGACCTG 1715 0.0 10.134401 6 CTGTAGG 2835 0.0 10.122636 1 TTTAGGA 1905 0.0 9.973597 2 >>END_MODULE