FastQCFastQC Report
Thu 26 May 2016
SRR1511859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693407
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78500.46356251037110396No Hit
GGTATCAACGCAGAGTACTTTTTTT49560.29266443329926No Hit
TATCAACGCAGAGTACTTTTTTTTT49170.29036138388467747No Hit
CTGTAGGACGTGGAATATGGCAAGA36660.21648664497076012No Hit
GTCCTACAGTGGACATTTCTAAATT36550.21583706693074967No Hit
CTTTAGGACGTGAAATATGGCGAGG31900.18837763160303458No Hit
GTCCTAAAGTGTGTATTTCTCATTT31890.18831857905394273No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22170.13091950133665445No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19700.11633352171096495No Hit
GTCCTACAGTGTGCATTTCTCATTT18580.10971963621267658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.014.7771851
CGTCTAA500.001025311213.9872091
TAGGACC7050.013.3977374
CGGTAGG758.801317E-613.3211541
CGTTAGG450.00824887213.3211531
AATCCCG1950.011.62545719
GGCGAGG10700.011.56434119
GTATCAA23500.011.3088091
TGTAGGA21350.011.1373572
TAGGACA5900.010.7261374
AGGACCT14000.010.6598135
GTAGGAC21850.010.5909573
GACGTGA12500.010.5785417
TTAGGAC14050.010.5546513
TGGCGAG23250.010.52228718
GGACGTG22150.010.4901556
GGACATG6050.010.4601996
CTGTAGG21800.010.3575021
CACCTTT6150.010.29011514
AGGACGT22950.010.2891115
Produced by FastQC (version 0.11.5)