FastQCFastQC Report
Thu 26 May 2016
SRR1511859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693407
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95990.5668454187327677No Hit
TATCAACGCAGAGTACTTTTTTTTT66860.39482534322817847No Hit
GGTATCAACGCAGAGTACTTTTTTT65180.3849045149807459No Hit
GTCCTACAGTGGACATTTCTAAATT36930.21808106379624034No Hit
CTGTAGGACGTGGAATATGGCAAGA36860.21766769595259733No Hit
CTTTAGGACGTGAAATATGGCGAGG34390.20308171632690783No Hit
GTCCTAAAGTGTGTATTTCTCATTT33220.19617256808316016No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30930.18264953434112413No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22460.1326320252603184No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19660.1160973115145975No Hit
GTCCTACAGTGTGCATTTCTCATTT18990.11214079072544285No Hit
CTGAAGGACCTGGAATATGGCGAGA17870.1055269052271545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.014.6272671
TACACCG400.0052794714.2499555
TAGGACC6850.012.7590844
AATCCCG2200.012.5212119
TGCCGGT951.0433268E-611.99677410
CTAGGAC2550.011.5489843
GTATTAA1501.6552804E-1011.4398341
ATGTCGA1001.9359777E-611.396934517
TCCAACG1104.976373E-711.22591218
CCCGCCC1104.9858863E-711.22425514
TGGTATC3150.011.1586952
CGGTTTC951.3643426E-510.99704313
TATGTCG951.3643426E-510.99704316
CGCCCTA1053.4773875E-610.85422516
GGCGAGG8500.010.83986219
CTCGAAC1158.830175E-710.73782818
TCGAACT909.5270145E-510.554275519
GTCCTAA10150.010.5193861
TGTAGGA21800.010.5022612
AGGACCT13450.010.4534995
Produced by FastQC (version 0.11.5)