##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511859_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1693407 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.702961544389506 31.0 31.0 34.0 27.0 34.0 2 30.91112532309126 31.0 31.0 34.0 27.0 34.0 3 31.00991492299252 31.0 31.0 34.0 27.0 34.0 4 34.609213260604214 37.0 35.0 37.0 32.0 37.0 5 34.22215214653063 37.0 35.0 37.0 30.0 37.0 6 34.17288047114486 37.0 35.0 37.0 30.0 37.0 7 34.00308962936849 37.0 35.0 37.0 30.0 37.0 8 33.9176642118522 37.0 35.0 37.0 30.0 37.0 9 35.38595978403302 39.0 35.0 39.0 30.0 39.0 10 35.183787476962124 38.0 35.0 39.0 29.0 39.0 11 35.16072509443979 39.0 35.0 39.0 29.0 39.0 12 34.9897325332894 38.0 35.0 39.0 28.0 39.0 13 34.969514121531326 38.0 35.0 39.0 28.0 39.0 14 35.942454471960964 39.0 36.0 41.0 27.0 41.0 15 35.87749607743443 39.0 36.0 41.0 27.0 41.0 16 35.775160962485685 39.0 36.0 41.0 27.0 41.0 17 35.73435151738477 39.0 36.0 41.0 27.0 41.0 18 35.66378962647491 39.0 36.0 41.0 27.0 41.0 19 35.691863798838675 39.0 36.0 41.0 27.0 41.0 20 35.55835189059689 39.0 35.0 41.0 27.0 41.0 21 35.44123651313594 39.0 35.0 41.0 27.0 41.0 22 35.52249990699224 39.0 36.0 41.0 27.0 41.0 23 35.41147639049561 39.0 36.0 41.0 27.0 41.0 24 35.28085038032794 39.0 35.0 41.0 27.0 41.0 25 34.84058587215005 39.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10168829683320624 1101 2 0.13176407809929103 1101 3 0.17942465971546184 1101 4 0.15262691266585193 1101 5 0.18309560666909874 1101 6 0.1699119978373318 1101 7 0.15935100091390808 1101 8 0.04910438337411449 1101 9 0.0639287957761141 1101 10 0.09995870944909768 1101 11 -0.12421793908639245 1101 12 -0.015793029959155547 1101 13 -0.06536356162225587 1101 14 -0.17935346070689206 1101 15 -0.3222749681636188 1101 16 -0.04154513338361454 1101 17 0.041631271384055424 1101 18 0.005651547946023072 1101 19 0.16791875082087415 1101 20 0.09442778189846734 1101 21 -0.0862456729726091 1101 22 -0.18185755716748275 1101 23 -0.22999882265796856 1101 24 -0.30411912524353824 1101 25 -0.2314388501365201 1102 1 0.549198888996667 1102 2 0.5616762146899781 1102 3 0.6105248812030872 1102 4 0.6569810068612796 1102 5 0.7297917495918043 1102 6 0.6964181802021301 1102 7 0.7293000188524061 1102 8 0.6484803270244726 1102 9 0.63322515686081 1102 10 0.7498320025643537 1102 11 0.8795242373454641 1102 12 0.8998338199281051 1102 13 1.0223759676001478 1102 14 1.2400612931624906 1102 15 1.2517853722488965 1102 16 1.2048382330952663 1102 17 1.3696300009895666 1102 18 1.4380703857013444 1102 19 1.381635186798981 1102 20 1.3318177863548186 1102 21 1.3619258901447182 1102 22 1.2316309061872843 1102 23 1.403178926149792 1102 24 1.260790716655535 1102 25 1.2758825463662333 1103 1 0.667821254194287 1103 2 0.601546644813638 1103 3 0.6144716617022432 1103 4 0.5453966749392265 1103 5 0.7425374908072371 1103 6 0.6469252932461274 1103 7 0.7693249305222096 1103 8 0.635661602401413 1103 9 0.9618434917524681 1103 10 0.8324107009782651 1103 11 0.8958335961700072 1103 12 1.0232069994989175 1103 13 0.8644816362214129 1103 14 1.0902737965176996 1103 15 1.025169053321136 1103 16 1.0304585717869372 1103 17 1.0780385815677107 1103 18 1.280023600093351 1103 19 1.180996906352938 1103 20 1.15111451823892 1103 21 1.0508268167907175 1103 22 1.3777663942070646 1103 23 1.1668582467877968 1103 24 1.2205651006147562 1103 25 1.2861075217748805 1104 1 0.29525077424012025 1104 2 0.22313843518733734 1104 3 0.26724040796667836 1104 4 0.2608858341592466 1104 5 0.334159304895266 1104 6 0.2821124214774642 1104 7 0.4277655530793538 1104 8 0.4535673497429258 1104 9 0.6164176815645135 1104 10 0.4617017222407469 1104 11 0.4470675994375881 1104 12 0.41204386858917985 1104 13 0.5071507235484418 1104 14 0.6191400166302614 1104 15 0.5495881605582937 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-1.0486263197261465 2315 8 -1.1294404130805589 2315 9 -1.3206526422936236 2315 10 -1.2597660863143716 2315 11 -1.143631707805305 2315 12 -1.207745459396861 2315 13 -1.1632611814990597 2315 14 -1.4067405623692295 2315 15 -1.5840453193733524 2315 16 -1.4110225058637198 2315 17 -1.497366075468456 2315 18 -1.52934946227046 2315 19 -1.3548792431018768 2315 20 -1.3999385996921987 2315 21 -1.4737786711621936 2315 22 -1.4709037533036735 2315 23 -1.3144073940789411 2315 24 -1.2216057066443895 2315 25 -1.2358100302554647 2316 1 -1.742252119031651 2316 2 -1.3125547606709738 2316 3 -1.210429304462945 2316 4 -0.888978911396336 2316 5 -1.2867204677208264 2316 6 -1.0703682264615324 2316 7 -1.2889050781443885 2316 8 -1.1858309651386563 2316 9 -1.388373768841447 2316 10 -1.5031077578166787 2316 11 -1.1810516040006078 2316 12 -1.4981502579248556 2316 13 -1.3565093341695302 2316 14 -1.5361749797182824 2316 15 -1.5729711523145014 2316 16 -1.5288672535832646 2316 17 -1.8270345385101692 2316 18 -1.9945123199608403 2316 19 -1.682110222924976 2316 20 -1.9042785776691176 2316 21 -1.9845573246253991 2316 22 -1.863273240174351 2316 23 -1.819567392047034 2316 24 -1.660166427767443 2316 25 -1.6856610102344476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28620.0 3 2.0 4 542.0 5 4617.0 6 5771.0 7 4583.0 8 3764.0 9 3355.0 10 3177.0 11 3062.0 12 2783.0 13 2738.0 14 2663.0 15 2705.0 16 2714.0 17 3040.0 18 3155.0 19 3489.0 20 4431.0 21 5592.0 22 7122.0 23 8855.0 24 11100.0 25 13188.0 26 15109.0 27 17142.0 28 20102.0 29 24372.0 30 31218.0 31 40346.0 32 52161.0 33 67463.0 34 88194.0 35 118489.0 36 176154.0 37 317384.0 38 594205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.5123818297471 21.295336265396145 14.526989976091773 23.665291928764983 2 14.21323993582169 22.085712412904872 41.99994449060385 21.701103160669586 3 18.11696774608821 26.688563351869927 31.352710836792337 23.841758065249525 4 12.242124899684482 18.775462721011547 39.8528528581729 29.12955952113107 5 12.054630425948153 39.00504200886486 36.70985294534594 12.230474619841038 6 29.27778141935164 39.74277890666568 18.673656126377182 12.305783547605508 7 25.85657198771471 33.022421662364685 23.613401857911303 17.507604492009303 8 23.33437856345226 38.900807661713934 21.47268790078227 16.292125874051543 9 25.451707711140912 15.702013750976581 21.866745560872253 36.97953297701025 10 14.299515710044897 30.218134211090426 35.644886314985115 19.837463763879565 11 32.27658796733449 23.399395420002396 26.446329795495117 17.87768681716799 12 22.389360620335218 25.920703056028465 33.31461367527122 18.3753226483651 13 28.040394305680792 23.370164408201926 26.93245037961931 21.656990906497967 14 19.594757787112016 24.133536710312406 29.273588688366114 26.99811681420946 15 21.495245974535358 33.12594078092272 24.755655314995153 20.623157929546764 16 20.08644117240638 29.79433166057047 29.892890423206246 20.2263367438169 17 19.894862841596854 30.207859067548437 29.61201884721157 20.285259243643143 18 19.64811704708386 28.496212362791155 32.5239576616094 19.331712928515582 19 21.946112186851714 27.85231193682322 29.94312648996963 20.258449386355437 20 22.00445610535447 28.44909699794556 30.544340492273857 19.002106404426105 21 21.23848549108395 27.787826553214913 29.512574354540877 21.461113601160264 22 21.407198623839395 29.452872227409006 29.788881231741687 19.351047917009907 23 20.383286475135627 29.137767825454837 30.32442879945577 20.154516899953762 24 21.466860288980712 27.917400673583604 31.06882233892842 19.54691669850726 25 20.997044416427258 28.640224872372528 30.57419813925198 19.788532571948235 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 321.0 1 321.0 2 694.5 3 1068.0 4 1068.0 5 1068.0 6 2544.0 7 4020.0 8 4020.0 9 4020.0 10 5624.0 11 7228.0 12 7228.0 13 7228.0 14 10562.5 15 13897.0 16 13897.0 17 13897.0 18 23010.0 19 32123.0 20 32123.0 21 32123.0 22 48078.5 23 64034.0 24 64034.0 25 64034.0 26 88938.0 27 113842.0 28 113842.0 29 113842.0 30 141173.5 31 168505.0 32 168505.0 33 168505.0 34 183069.5 35 197634.0 36 197634.0 37 197634.0 38 203092.5 39 208551.0 40 208551.0 41 208551.0 42 211119.0 43 213687.0 44 213687.0 45 213687.0 46 210556.5 47 207426.0 48 207426.0 49 207426.0 50 191951.0 51 176476.0 52 176476.0 53 176476.0 54 149780.5 55 123085.0 56 123085.0 57 123085.0 58 101474.0 59 79863.0 60 79863.0 61 79863.0 62 62658.0 63 45453.0 64 45453.0 65 45453.0 66 33835.5 67 22218.0 68 22218.0 69 22218.0 70 15726.0 71 9234.0 72 9234.0 73 9234.0 74 6273.5 75 3313.0 76 3313.0 77 3313.0 78 2193.0 79 1073.0 80 1073.0 81 1073.0 82 669.5 83 266.0 84 266.0 85 266.0 86 169.5 87 73.0 88 73.0 89 73.0 90 43.5 91 14.0 92 14.0 93 14.0 94 8.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3862036710607668 2 0.0 3 0.0 4 0.0 5 0.02545164865859182 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 5.9052549091860376E-5 17 0.0 18 1.7715764727558111E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004487993730981388 25 1.1810509818372075E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1693407.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.23761918514198 #Duplication Level Percentage of deduplicated Percentage of total 1 86.6600303294012 64.33434330166939 2 9.796652081647634 14.54560253053371 3 1.9912577399095628 4.4347870138461785 4 0.6030483440151988 1.790754932529233 5 0.27164671128710455 1.008320255271414 6 0.16589647814079236 0.7389455741023424 7 0.09987057764316545 0.518990773761143 8 0.06513319750201829 0.386826680997238 9 0.04729040070424475 0.3159654082735043 >10 0.2564650848490605 3.425021885118934 >50 0.021091111576732845 1.0740826657978426 >100 0.017565111110945344 2.6054357811790574 >500 0.0023840189485006442 1.3279101328896377 >1k 0.0014304113691003865 2.1477585098396266 >5k 2.3840189485006445E-4 1.3452545541908751 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9599 0.5668454187327677 No Hit TATCAACGCAGAGTACTTTTTTTTT 6686 0.39482534322817847 No Hit GGTATCAACGCAGAGTACTTTTTTT 6518 0.3849045149807459 No Hit GTCCTACAGTGGACATTTCTAAATT 3693 0.21808106379624034 No Hit CTGTAGGACGTGGAATATGGCAAGA 3686 0.21766769595259733 No Hit CTTTAGGACGTGAAATATGGCGAGG 3439 0.20308171632690783 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3322 0.19617256808316016 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3093 0.18264953434112413 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2246 0.1326320252603184 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1966 0.1160973115145975 No Hit GTCCTACAGTGTGCATTTCTCATTT 1899 0.11214079072544285 No Hit CTGAAGGACCTGGAATATGGCGAGA 1787 0.1055269052271545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.9052549091860376E-5 0.0 9 0.0 0.0 0.0 5.9052549091860376E-5 0.0 10 0.0 0.0 0.0 5.9052549091860376E-5 0.0 11 0.0 0.0 0.0 5.9052549091860376E-5 0.0 12 0.0 0.0 0.0 5.9052549091860376E-5 5.9052549091860376E-5 13 0.0 0.0 0.0 5.9052549091860376E-5 1.1810509818372075E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1310 0.0 14.627267 1 TACACCG 40 0.00527947 14.249955 5 TAGGACC 685 0.0 12.759084 4 AATCCCG 220 0.0 12.52121 19 TGCCGGT 95 1.0433268E-6 11.996774 10 CTAGGAC 255 0.0 11.548984 3 GTATTAA 150 1.6552804E-10 11.439834 1 ATGTCGA 100 1.9359777E-6 11.3969345 17 TCCAACG 110 4.976373E-7 11.225912 18 CCCGCCC 110 4.9858863E-7 11.224255 14 TGGTATC 315 0.0 11.158695 2 CGGTTTC 95 1.3643426E-5 10.997043 13 TATGTCG 95 1.3643426E-5 10.997043 16 CGCCCTA 105 3.4773875E-6 10.854225 16 GGCGAGG 850 0.0 10.839862 19 CTCGAAC 115 8.830175E-7 10.737828 18 TCGAACT 90 9.5270145E-5 10.5542755 19 GTCCTAA 1015 0.0 10.519386 1 TGTAGGA 2180 0.0 10.502261 2 AGGACCT 1345 0.0 10.453499 5 >>END_MODULE