FastQCFastQC Report
Thu 26 May 2016
SRR1511858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836321
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75180.4094055451089434No Hit
TATCAACGCAGAGTACTTTTTTTTT51890.2825758677268299No Hit
GGTATCAACGCAGAGTACTTTTTTT51600.2809966231394184No Hit
GTCCTACAGTGGACATTTCTAAATT37070.2018710236391132No Hit
CTGTAGGACGTGGAATATGGCAAGA35040.190816311527233No Hit
GTCCTAAAGTGTGTATTTCTCATTT34980.19048957126776855No Hit
CTTTAGGACGTGAAATATGGCGAGG33890.18455378988749788No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22440.12220085703970057No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21350.11626507565942992No Hit
GTCCTACAGTGTGCATTTCTCATTT18620.10139839385379788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC653.58117E-614.53122710
GGTATCA11500.014.3345191
GTGCGCC802.1338947E-612.98728511
TAGGACC7750.012.918734
TTACACG701.15152456E-412.1439534
CGGTAGG709.790936E-411.4179731
GCGTGCG1002.0688076E-611.3343579
GGCGTGC1105.3544863E-711.1626258
CTAGTAC1105.3544863E-711.1626253
ATCCCGT855.646293E-511.11211510
GAGCCGT600.00613436511.0195156
GCGCCAC901.0089898E-410.49477513
TGTAGGA22600.010.4327942
GGCGAGG10150.010.42296519
AGGACGT22450.010.3498595
GGACCTG12700.010.3377676
GGCCTAC1457.747076E-810.3352351
GTATCAA23350.010.3116271
GGACGTG21950.010.2844026
AATCCCG1752.6193447E-1010.25545319
Produced by FastQC (version 0.11.5)