##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511858_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1836321 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.85440944148654 31.0 30.0 34.0 25.0 34.0 2 27.05945801414894 31.0 27.0 34.0 10.0 34.0 3 28.836303130008314 31.0 28.0 34.0 16.0 34.0 4 33.272555288536154 35.0 33.0 37.0 28.0 37.0 5 33.65899589450864 35.0 35.0 37.0 28.0 37.0 6 33.87875485821923 37.0 35.0 37.0 30.0 37.0 7 33.87848148553548 37.0 35.0 37.0 30.0 37.0 8 33.902553529584424 37.0 35.0 37.0 30.0 37.0 9 35.49092288330853 39.0 35.0 39.0 30.0 39.0 10 35.37289286568089 39.0 35.0 39.0 30.0 39.0 11 35.373050245572536 39.0 35.0 39.0 30.0 39.0 12 35.27383992232295 39.0 35.0 39.0 30.0 39.0 13 35.25866338183793 39.0 35.0 39.0 30.0 39.0 14 36.38474918056266 40.0 36.0 41.0 29.0 41.0 15 36.32716828920434 40.0 36.0 41.0 29.0 41.0 16 36.28544900374172 40.0 36.0 41.0 29.0 41.0 17 36.25554954716523 40.0 36.0 41.0 29.0 41.0 18 36.23510105259375 40.0 36.0 41.0 29.0 41.0 19 36.274904551001704 40.0 37.0 41.0 29.0 41.0 20 36.21153109941018 40.0 36.0 41.0 29.0 41.0 21 36.118556069445376 40.0 36.0 41.0 28.0 41.0 22 36.02525157638561 40.0 36.0 41.0 28.0 41.0 23 35.923163216017244 40.0 36.0 41.0 27.0 41.0 24 35.81266020483347 40.0 36.0 41.0 27.0 41.0 25 35.71358438965736 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38795931880836676 1101 2 3.0672726858987325 1101 3 1.6929030691050713 1101 4 0.6748601124833158 1101 5 -0.0783126711960449 1101 6 0.012951124414684045 1101 7 -0.00792278644880895 1101 8 -0.09312576131396355 1101 9 -0.08825416018544985 1101 10 -0.20331805852483598 1101 11 -0.10310509385024602 1101 12 -0.15645933006772594 1101 13 -0.176472560844104 1101 14 -0.15541005856870527 1101 15 0.044622917750970714 1101 16 -0.05876333805070999 1101 17 0.06847727079613719 1101 18 0.16748671929816794 1101 19 -0.02855672929855757 1101 20 -0.006019156570523876 1101 21 -0.00805772306511443 1101 22 0.013132247000747554 1101 23 -0.1876638247652025 1101 24 -0.24233676160753959 1101 25 -0.02077385330547088 1102 1 1.491675489879725 1102 2 4.125040792162572 1102 3 2.3861534897915035 1102 4 1.5968673075035298 1102 5 1.253873129332817 1102 6 1.2435019436498322 1102 7 1.1629500467500264 1102 8 1.1037921239739248 1102 9 1.1027414928667838 1102 10 1.159610037676721 1102 11 1.0636029174917994 1102 12 1.116073954140461 1102 13 1.230207273316644 1102 14 1.2605983206188398 1102 15 1.230011744361171 1102 16 1.2398401190619524 1102 17 1.32769163822784 1102 18 1.2684455319767878 1102 19 1.2504667966939849 1102 20 1.3398742355734328 1102 21 1.3226012901264568 1102 22 1.531274185888087 1102 23 1.525400987985286 1102 24 1.0688284988168135 1102 25 1.166683951574882 1103 1 0.996056022071798 1103 2 3.5870375885785855 1103 3 2.0940217871964393 1103 4 1.2280692363181416 1103 5 0.7108982843693497 1103 6 0.49910150041144163 1103 7 0.5667107728231926 1103 8 0.3483175212683207 1103 9 0.47444665841645417 1103 10 0.6978668894941293 1103 11 0.7096269474318149 1103 12 0.4941910478213174 1103 13 0.7758532449848801 1103 14 0.9434996075946103 1103 15 0.592843981895605 1103 16 0.5949411856425684 1103 17 0.7600653130773267 1103 18 0.9990167063171072 1103 19 0.9414430057817285 1103 20 1.0299531555351749 1103 21 0.960469815338783 1103 22 0.9803995244965762 1103 23 0.9846399548821552 1103 24 1.1533851635261527 1103 25 1.1185020075889796 1104 1 1.0130808818960197 1104 2 3.658313346999254 1104 3 1.9509478516122805 1104 4 1.201680670671962 1104 5 0.9991786247348742 1104 6 0.8254505966138481 1104 7 0.5887721838541609 1104 8 0.6844378559981692 1104 9 0.7217567944263834 1104 10 0.7182583381543921 1104 11 0.6864538740854726 1104 12 0.6065150277633364 1104 13 0.6545609120313216 1104 14 0.6558216782557764 1104 15 0.744182470067777 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-1.1396651541760363 2315 8 -1.0340694730144548 2315 9 -1.204636115845723 2315 10 -1.256730920602358 2315 11 -1.2674764062336052 2315 12 -1.3537964455934386 2315 13 -1.24648693663584 2315 14 -1.3308404880814209 2315 15 -1.295305250735204 2315 16 -1.3810584858093478 2315 17 -1.2037359229488445 2315 18 -1.2168238804131946 2315 19 -1.4084775633679882 2315 20 -1.3316373979459541 2315 21 -1.4868720593380687 2315 22 -1.3089792612517286 2315 23 -1.5244458410432529 2315 24 -1.4666242305555741 2315 25 -1.4994651120370648 2316 1 -1.0090001873337648 2316 2 -0.9594126006189754 2316 3 -0.49029260169319855 2316 4 -0.5279520377467577 2316 5 -0.5318390429850695 2316 6 -0.783069098332625 2316 7 -0.6411436752271484 2316 8 -0.9391337205230172 2316 9 -0.8006525973615908 2316 10 -0.9217929925435513 2316 11 -1.16615436218337 2316 12 -1.2939517301285264 2316 13 -1.0152234417461514 2316 14 -1.1290019971059237 2316 15 -1.787154155305167 2316 16 -1.4179502827369745 2316 17 -1.376279807510869 2316 18 -1.3197851097999234 2316 19 -1.2291027158397583 2316 20 -1.3544565054353654 2316 21 -1.4232760831364146 2316 22 -1.1993490986030295 2316 23 -1.308857809758777 2316 24 -1.3102776271715229 2316 25 -1.2102921553408663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54525.0 3 1.0 4 483.0 5 2450.0 6 3066.0 7 2737.0 8 2513.0 9 2543.0 10 2441.0 11 2411.0 12 2336.0 13 2233.0 14 2266.0 15 2357.0 16 2516.0 17 2655.0 18 3036.0 19 3767.0 20 4972.0 21 6278.0 22 7711.0 23 9201.0 24 10874.0 25 12545.0 26 14210.0 27 16614.0 28 19401.0 29 23662.0 30 29719.0 31 38527.0 32 50522.0 33 68426.0 34 94442.0 35 137939.0 36 215938.0 37 294619.0 38 686385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99935632168886 20.084723749279128 13.321908315594062 24.594011613437956 2 15.584042301954392 21.94782528555547 40.86366490216521 21.604467510324927 3 17.92039627058668 26.020614042969616 30.687989735999317 25.370999950444396 4 11.608482394962536 17.84459253039093 40.0854752518759 30.461449822770636 5 12.175817617049827 38.775164568983925 36.55680516165009 12.492212652316164 6 29.499479117598526 38.88968456799435 19.080360745779437 12.530475568627686 7 26.14412186104717 32.58248421708405 23.77242323101462 17.50097069085416 8 23.623375215988926 38.121929662624346 21.640170754459596 16.61452436692713 9 25.853976510642745 15.360222967553058 21.658359295569785 37.12744122623441 10 14.452701896890577 29.648628970642932 35.70552207375508 20.193147058711414 11 32.345924269231794 23.070095043295808 26.436663306687663 18.147317380784735 12 22.613257703854607 25.49096808237775 33.184993255536476 18.71078095823116 13 28.23628587205484 23.1631449454833 26.68366807927603 21.916901103185833 14 19.8249652430049 23.709416817647895 29.156775966729125 27.308841972618076 15 21.76438650976599 32.81904416493631 24.364912234843473 21.051657090454228 16 20.526912233754338 29.071932412688195 29.760101855830218 20.64105349772725 17 20.262329862975502 29.759398447106538 29.30680700577352 20.671464684144432 18 19.92064568231807 28.012150381115287 32.134305494518664 19.932898442047986 19 22.300942593245065 27.504183645852 29.35696832300741 20.837905437895525 20 22.3301980704846 28.100446764064575 30.0618194254148 19.507535740036026 21 21.599512064956354 27.496610049501445 28.953771422036585 21.950106463505616 22 21.80252046152732 29.190562728080206 29.189201258433624 19.817715551958855 23 20.72214150505686 28.87765504412095 29.740905160114057 20.659298290708136 24 21.79506798229253 27.523203072286627 30.59587090688983 20.08585803853101 25 21.313733146218855 28.298832059310282 30.003338218132136 20.38409657633873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 412.0 1 412.0 2 707.5 3 1003.0 4 1003.0 5 1003.0 6 2462.0 7 3921.0 8 3921.0 9 3921.0 10 5438.5 11 6956.0 12 6956.0 13 6956.0 14 10468.0 15 13980.0 16 13980.0 17 13980.0 18 22982.0 19 31984.0 20 31984.0 21 31984.0 22 48058.5 23 64133.0 24 64133.0 25 64133.0 26 89877.5 27 115622.0 28 115622.0 29 115622.0 30 144416.0 31 173210.0 32 173210.0 33 173210.0 34 189197.0 35 205184.0 36 205184.0 37 205184.0 38 212694.0 39 220204.0 40 220204.0 41 220204.0 42 225352.0 43 230500.0 44 230500.0 45 230500.0 46 228395.5 47 226291.0 48 226291.0 49 226291.0 50 212599.0 51 198907.0 52 198907.0 53 198907.0 54 170875.5 55 142844.0 56 142844.0 57 142844.0 58 119342.0 59 95840.0 60 95840.0 61 95840.0 62 76429.0 63 57018.0 64 57018.0 65 57018.0 66 42962.0 67 28906.0 68 28906.0 69 28906.0 70 20711.0 71 12516.0 72 12516.0 73 12516.0 74 8625.5 75 4735.0 76 4735.0 77 4735.0 78 3133.5 79 1532.0 80 1532.0 81 1532.0 82 979.0 83 426.0 84 426.0 85 426.0 86 283.0 87 140.0 88 140.0 89 140.0 90 89.0 91 38.0 92 38.0 93 38.0 94 24.0 95 10.0 96 10.0 97 10.0 98 9.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.458250491063382 3 0.0 4 0.0 5 4.9011038919666E-4 6 7.623939387503601E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.6337012973222E-4 14 0.0 15 0.0 16 0.0 17 3.8119696937518005E-4 18 0.0 19 2.1782683964296004E-4 20 2.722835495537E-4 21 5.990238090181401E-4 22 0.0038119696937518005 23 2.722835495537E-4 24 0.0018515281369651602 25 6.5348051892888E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1836321.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.8037241492955 #Duplication Level Percentage of deduplicated Percentage of total 1 87.2311021585166 66.1244240526321 2 9.346078599124699 14.169351280113657 3 1.9381948617658094 4.407671659466321 4 0.5705098099882124 1.7298707304325378 5 0.2693726943138082 1.020972670655821 6 0.1502073860041205 0.6831767552305861 7 0.09559694467573307 0.5072623096600314 8 0.07027921358572173 0.4261940896065177 9 0.045603375548871894 0.31112151303450714 >10 0.2437947322521263 3.3210345747185945 >50 0.02052699484750311 1.0891594260770199 >100 0.015030927942672061 2.229563623142367 >500 0.002395606810371087 1.2541124143025928 >1k 0.0010889121865323116 1.74288057857796 >5k 2.177824373064623E-4 0.9832043223494699 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7518 0.4094055451089434 No Hit TATCAACGCAGAGTACTTTTTTTTT 5189 0.2825758677268299 No Hit GGTATCAACGCAGAGTACTTTTTTT 5160 0.2809966231394184 No Hit GTCCTACAGTGGACATTTCTAAATT 3707 0.2018710236391132 No Hit CTGTAGGACGTGGAATATGGCAAGA 3504 0.190816311527233 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3498 0.19048957126776855 No Hit CTTTAGGACGTGAAATATGGCGAGG 3389 0.18455378988749788 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2244 0.12220085703970057 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2135 0.11626507565942992 No Hit GTCCTACAGTGTGCATTTCTCATTT 1862 0.10139839385379788 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.4456709910740004E-5 0.0 4 0.0 0.0 0.0 5.4456709910740004E-5 0.0 5 0.0 0.0 0.0 5.4456709910740004E-5 0.0 6 0.0 0.0 0.0 5.4456709910740004E-5 0.0 7 0.0 0.0 0.0 5.4456709910740004E-5 0.0 8 0.0 0.0 0.0 5.4456709910740004E-5 0.0 9 0.0 0.0 0.0 1.0891341982148001E-4 0.0 10 0.0 0.0 0.0 1.0891341982148001E-4 0.0 11 0.0 0.0 0.0 1.0891341982148001E-4 0.0 12 0.0 0.0 0.0 1.0891341982148001E-4 0.0 13 0.0 0.0 0.0 1.0891341982148001E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 65 3.58117E-6 14.531227 10 GGTATCA 1150 0.0 14.334519 1 GTGCGCC 80 2.1338947E-6 12.987285 11 TAGGACC 775 0.0 12.91873 4 TTACACG 70 1.15152456E-4 12.143953 4 CGGTAGG 70 9.790936E-4 11.417973 1 GCGTGCG 100 2.0688076E-6 11.334357 9 GGCGTGC 110 5.3544863E-7 11.162625 8 CTAGTAC 110 5.3544863E-7 11.162625 3 ATCCCGT 85 5.646293E-5 11.112115 10 GAGCCGT 60 0.006134365 11.019515 6 GCGCCAC 90 1.0089898E-4 10.494775 13 TGTAGGA 2260 0.0 10.432794 2 GGCGAGG 1015 0.0 10.422965 19 AGGACGT 2245 0.0 10.349859 5 GGACCTG 1270 0.0 10.337767 6 GGCCTAC 145 7.747076E-8 10.335235 1 GTATCAA 2335 0.0 10.311627 1 GGACGTG 2195 0.0 10.284402 6 AATCCCG 175 2.6193447E-10 10.255453 19 >>END_MODULE