FastQCFastQC Report
Thu 26 May 2016
SRR1511858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836321
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94600.5151604757556004No Hit
TATCAACGCAGAGTACTTTTTTTTT69930.3808157724058049No Hit
GGTATCAACGCAGAGTACTTTTTTT67190.3658946338902621No Hit
GTCCTACAGTGGACATTTCTAAATT39710.21624759505554855No Hit
GTCCTAAAGTGTGTATTTCTCATTT37990.2068810409509013No Hit
CTGTAGGACGTGGAATATGGCAAGA36490.1987125344642903No Hit
CTTTAGGACGTGAAATATGGCGAGG36110.19664317948768217No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31030.16897917085302624No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25720.1400626578904233No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21330.11615616223960842No Hit
GTCCTACAGTGTGCATTTCTCATTT20680.11261647609541034No Hit
CTGAAGGACCTGGAATATGGCGAGA18910.10297763844120936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.013.7266381
TAGGACC8200.013.3233044
CCAACGA853.954792E-612.29122819
TGAACCG550.003068580512.091035
GTGGTAT4450.011.7837341
GGCGAGG10200.011.639420519
TGGCGAA1001.9354811E-611.39732218
CTCGAAC1354.7457434E-911.25661318
GGCGAAA1053.4765017E-610.85459119
GCCGGTT700.001494893510.85429511
ACTGTTC4600.010.7363158
AGGACCT16600.010.5874565
TGTAGGA23800.010.537922
TGGCGAG24000.010.48711818
GGACCTG16350.010.4559746
TAGTACC1002.3987568E-510.4501054
TTAGGAC14750.010.36959653
TGGTATC4500.010.3445482
GGACGTG22150.010.3336326
AGGACGT22550.010.2373535
Produced by FastQC (version 0.11.5)