##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511858_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1836321 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.764902759375946 31.0 31.0 34.0 27.0 34.0 2 30.97211271885471 31.0 31.0 34.0 27.0 34.0 3 31.07485292604071 31.0 31.0 34.0 27.0 34.0 4 34.66516638430863 37.0 35.0 37.0 32.0 37.0 5 34.28917819923641 37.0 35.0 37.0 30.0 37.0 6 34.23090352939383 37.0 35.0 37.0 30.0 37.0 7 34.06541775648157 37.0 35.0 37.0 30.0 37.0 8 33.97380033229484 37.0 35.0 37.0 30.0 37.0 9 35.46086768054169 39.0 35.0 39.0 30.0 39.0 10 35.26773750341035 39.0 35.0 39.0 29.0 39.0 11 35.237652894020165 39.0 35.0 39.0 29.0 39.0 12 35.07222157781782 38.0 35.0 39.0 28.0 39.0 13 35.06140701979665 38.0 35.0 39.0 29.0 39.0 14 36.04868103125761 39.0 36.0 41.0 28.0 41.0 15 35.98704964981613 39.0 36.0 41.0 27.0 41.0 16 35.88413844856101 39.0 36.0 41.0 27.0 41.0 17 35.83955092818739 39.0 36.0 41.0 27.0 41.0 18 35.77510685767902 39.0 36.0 41.0 27.0 41.0 19 35.79913969289683 39.0 36.0 41.0 27.0 41.0 20 35.67234541237616 39.0 36.0 41.0 27.0 41.0 21 35.55630088639187 39.0 36.0 41.0 27.0 41.0 22 35.63303365805869 39.0 36.0 41.0 27.0 41.0 23 35.51978602869542 39.0 36.0 41.0 27.0 41.0 24 35.39696218689434 39.0 36.0 41.0 27.0 41.0 25 34.9581472955981 39.0 35.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5148476857333577 1101 2 0.4201612883411876 1101 3 0.4506352104437177 1101 4 0.4179143479518288 1101 5 0.520966908025855 1101 6 0.5384908992633086 1101 7 0.23578790203771405 1101 8 0.47163113601421713 1101 9 0.35853987982157776 1101 10 0.5123811883451523 1101 11 0.17044186062467048 1101 12 0.4516810446615551 1101 13 0.39449627837905865 1101 14 0.32270541607638137 1101 15 0.08432765181365198 1101 16 0.3372762755587857 1101 17 0.30578285450951626 1101 18 0.26288072569450804 1101 19 0.2503442280662398 1101 20 0.10927648489534647 1101 21 0.25238811429750996 1101 22 0.0675340971443461 1101 23 0.028890850417518266 1101 24 0.11924353781881791 1101 25 0.18612308590572013 1102 1 0.5821864737726372 1102 2 0.5724506494316266 1102 3 0.6066253561060684 1102 4 0.6061561316134529 1102 5 0.7435819918892506 1102 6 0.7345204846058664 1102 7 0.7501816059240056 1102 8 0.7604737607347047 1102 9 0.783306003285503 1102 10 1.0052683341285729 1102 11 0.9652885346810436 1102 12 1.0666654715004995 1102 13 1.162913063410123 1102 14 1.1444017061157368 1102 15 1.2646916175390928 1102 16 1.243994663193206 1102 17 1.3619557889940808 1102 18 1.4563444988619167 1102 19 1.5453097026455467 1102 20 1.4092204166309301 1102 21 1.4876494353244354 1102 22 1.2322596574693705 1102 23 1.41784655369743 1102 24 1.3964210327780862 1102 25 1.5673670477513895 1103 1 0.5166377860622369 1103 2 0.4629912363790787 1103 3 0.4900287713809277 1103 4 0.5138417750894462 1103 5 0.5683610442172906 1103 6 0.5202190083799536 1103 7 0.6183168255932401 1103 8 0.46107976240551807 1103 9 0.6591934745928185 1103 10 0.5415942632682302 1103 11 0.6205707251409436 1103 12 0.7465858817170101 1103 13 0.6395167339652659 1103 14 0.90925279318202 1103 15 0.9371079841743537 1103 16 0.6297552049803912 1103 17 0.7907032461365446 1103 18 0.7443117462787683 1103 19 0.7721263051124581 1103 20 0.7098381146814106 1103 21 0.6726718811530219 1103 22 0.8486347250040609 1103 23 0.8454869349042013 1103 24 0.9870334640576033 1103 25 0.9183573808976035 1104 1 0.3380291711895289 1104 2 0.285496917312571 1104 3 0.2885400590163911 1104 4 0.2722565189511883 1104 5 0.45378740473626067 1104 6 0.442178141210924 1104 7 0.4810678425321768 1104 8 0.493016105665248 1104 9 0.6587283342754944 1104 10 0.628850702400193 1104 11 0.52596816334691 1104 12 0.556632846734054 1104 13 0.7523884799993112 1104 14 0.6860512781347481 1104 15 0.7564182531755606 1104 16 0.9363310644941905 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0.9103410135515446 1107 16 0.9917724568842345 1107 17 1.0276616134040566 1107 18 1.1357790054794492 1107 19 1.1020202421525838 1107 20 1.2429982071393084 1107 21 1.1144288951331234 1107 22 0.90096162928063 1107 23 1.0093090931026225 1107 24 0.871042734955175 1107 25 0.7604285508855071 1108 1 0.3441429768179667 1108 2 0.27891837682384946 1108 3 0.365936699366916 1108 4 0.3829992978694108 1108 5 0.4956789558981285 1108 6 0.5028996309314522 1108 7 0.5825414950686891 1108 8 0.4875011330557655 1108 9 0.5443208994691986 1108 10 0.583395291970632 1108 11 0.7042512192863555 1108 12 0.6076677515035414 1108 13 0.7805202518076584 1108 14 0.8822993246427231 1108 15 0.7372984128326507 1108 16 0.9224850579432982 1108 17 0.7735316568325246 1108 18 0.8188523916526407 1108 19 0.7353782378664349 1108 20 0.7175985064895869 1108 21 0.6589481771460299 1108 22 0.7860703705354055 1108 23 0.8738873947433774 1108 24 0.866618530671289 1108 25 0.805740997733821 1109 1 0.015007366898068142 1109 2 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-0.758082158700077 2315 7 -0.9097366385355983 2315 8 -0.8309769077868765 2315 9 -0.7471432605367738 2315 10 -0.8523318944625871 2315 11 -0.9216611661618614 2315 12 -1.0520756112239624 2315 13 -1.0510709346570053 2315 14 -1.0685215274673112 2315 15 -1.251668454359006 2315 16 -1.5117826556983616 2315 17 -1.4878883922603947 2315 18 -1.3286860346617928 2315 19 -1.3046701770296707 2315 20 -1.3601736320004036 2315 21 -1.2478018046750492 2315 22 -1.1612277009264318 2315 23 -1.2534802872571262 2315 24 -1.0258283229991818 2315 25 -1.318684892198199 2316 1 -1.557552962334185 2316 2 -1.0879810101567884 2316 3 -1.0644783050117965 2316 4 -0.9087241298494462 2316 5 -1.1058199413157155 2316 6 -1.010868978584945 2316 7 -1.0108750337497128 2316 8 -0.9190782837649678 2316 9 -1.1103185056749751 2316 10 -1.2436659450959624 2316 11 -1.1147398124673984 2316 12 -1.0243523743438203 2316 13 -1.2338261041327883 2316 14 -1.5095647893983397 2316 15 -1.3944054728668078 2316 16 -1.289470846827875 2316 17 -1.4401615415716762 2316 18 -1.4600937177826694 2316 19 -1.2801479370873494 2316 20 -1.2141605185177156 2316 21 -1.5264476095621546 2316 22 -1.5219759140482907 2316 23 -1.3694573718876129 2316 24 -1.3250229989579765 2316 25 -1.3997907722379566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31010.0 3 0.0 4 579.0 5 4919.0 6 6084.0 7 5050.0 8 3955.0 9 3606.0 10 3336.0 11 3239.0 12 2983.0 13 2920.0 14 2790.0 15 2841.0 16 2976.0 17 3137.0 18 3337.0 19 3758.0 20 4593.0 21 5843.0 22 7569.0 23 9545.0 24 11496.0 25 13869.0 26 16046.0 27 18161.0 28 21380.0 29 25935.0 30 32991.0 31 42329.0 32 54573.0 33 71070.0 34 92993.0 35 124752.0 36 184265.0 37 333530.0 38 678861.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.99812137369355 21.009832716966027 14.038685705162832 23.953360204177592 2 14.37335847055063 21.999639496580393 41.63476864883645 21.99223338403253 3 17.964887402583756 26.714065786972974 31.17309010788419 24.147956702559085 4 12.21812526241327 18.715191951733928 39.61605841244532 29.45062437340748 5 12.138423224580684 38.9896658111129 36.56179596567908 12.310114998627338 6 29.445886639645245 39.382275756798514 18.74688575690198 12.424951846654261 7 26.032648975859885 32.78054327102941 23.794696025368115 17.39211172774259 8 23.706911808992 38.381143601799465 21.495969386615958 16.415975202592577 9 25.904294510600273 15.230942738224961 21.576020750184743 37.28874200099002 10 14.378531857992149 29.95854210674495 35.65199112791282 20.010934907350077 11 32.49747729291339 23.021410744635606 26.327368689896808 18.1537432725542 12 22.433387191019435 25.806381346180764 33.23857865808865 18.52165280471116 13 28.355772220652053 23.106689952355826 26.712323172255832 21.825214654736293 14 19.855951110944112 23.690139142339493 29.230564808658183 27.223344938058215 15 21.81492233656316 32.86810966056588 24.36169928895874 20.95526871391222 16 20.38288533588917 29.37222270628213 29.62408512677529 20.62080683105341 17 20.280713448247884 29.79784035579836 29.343181284753587 20.57826491120017 18 19.95834059423194 28.086553541866344 32.20751285179054 19.747593012111178 19 22.249976992040065 27.55101096159114 29.570810332180486 20.628201714188314 20 22.391401067678256 28.148183242472314 30.10557522350395 19.35484046634548 21 21.58974384108225 27.486752043896466 29.108636235168035 21.81486787985325 22 21.865349249940504 29.127587170216973 29.3548894773844 19.652174102458122 23 20.745338097206318 28.80710943239227 29.9308780981103 20.516674372291117 24 21.803056030863104 27.696191887979666 30.583671615553104 19.91708046560413 25 21.358792934350802 28.278770432838268 30.17446296154104 20.187973671269894 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 414.0 1 414.0 2 718.5 3 1023.0 4 1023.0 5 1023.0 6 2630.0 7 4237.0 8 4237.0 9 4237.0 10 5771.5 11 7306.0 12 7306.0 13 7306.0 14 10686.5 15 14067.0 16 14067.0 17 14067.0 18 23425.5 19 32784.0 20 32784.0 21 32784.0 22 49666.0 23 66548.0 24 66548.0 25 66548.0 26 92807.0 27 119066.0 28 119066.0 29 119066.0 30 146960.0 31 174854.0 32 174854.0 33 174854.0 34 190934.0 35 207014.0 36 207014.0 37 207014.0 38 213710.0 39 220406.0 40 220406.0 41 220406.0 42 225212.0 43 230018.0 44 230018.0 45 230018.0 46 227792.0 47 225566.0 48 225566.0 49 225566.0 50 211271.0 51 196976.0 52 196976.0 53 196976.0 54 168724.5 55 140473.0 56 140473.0 57 140473.0 58 117095.0 59 93717.0 60 93717.0 61 93717.0 62 74500.5 63 55284.0 64 55284.0 65 55284.0 66 41635.0 67 27986.0 68 27986.0 69 27986.0 70 20067.5 71 12149.0 72 12149.0 73 12149.0 74 8337.0 75 4525.0 76 4525.0 77 4525.0 78 2955.5 79 1386.0 80 1386.0 81 1386.0 82 893.5 83 401.0 84 401.0 85 401.0 86 249.5 87 98.0 88 98.0 89 98.0 90 59.0 91 20.0 92 20.0 93 20.0 94 11.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3988954000961706 2 0.0 3 0.0 4 0.0 5 0.025758023787780023 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 5.445670991074001E-5 17 0.0 18 5.445670991074001E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0045199069225914205 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1836321.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.13400769127152 #Duplication Level Percentage of deduplicated Percentage of total 1 86.55803652654399 64.16894145600173 2 9.826304972615302 14.569267368332847 3 2.016107564602417 4.483864011019988 4 0.6387168432197492 1.894025574711902 5 0.2779409322695336 1.0302437605294388 6 0.1537497844220422 0.6838852620525009 7 0.09836981059824831 0.5104783806835623 8 0.07151098266276247 0.42411165909852944 9 0.05147803088647399 0.34346454639024376 >10 0.26534000034279737 3.5554502852701635 >50 0.021547704891814727 1.1248430113268002 >100 0.017011599650344395 2.5566050816705364 >500 0.0023458096872590287 1.2964020130930753 >1k 0.0013195179490832034 2.0991332700789402 >5k 2.199196581805339E-4 1.2592843197397918 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9460 0.5151604757556004 No Hit TATCAACGCAGAGTACTTTTTTTTT 6993 0.3808157724058049 No Hit GGTATCAACGCAGAGTACTTTTTTT 6719 0.3658946338902621 No Hit GTCCTACAGTGGACATTTCTAAATT 3971 0.21624759505554855 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3799 0.2068810409509013 No Hit CTGTAGGACGTGGAATATGGCAAGA 3649 0.1987125344642903 No Hit CTTTAGGACGTGAAATATGGCGAGG 3611 0.19664317948768217 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3103 0.16897917085302624 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2572 0.1400626578904233 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2133 0.11615616223960842 No Hit GTCCTACAGTGTGCATTTCTCATTT 2068 0.11261647609541034 No Hit CTGAAGGACCTGGAATATGGCGAGA 1891 0.10297763844120936 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.4456709910740004E-5 2 0.0 0.0 0.0 0.0 5.4456709910740004E-5 3 0.0 0.0 0.0 0.0 5.4456709910740004E-5 4 0.0 0.0 0.0 0.0 5.4456709910740004E-5 5 0.0 0.0 0.0 0.0 5.4456709910740004E-5 6 0.0 0.0 0.0 0.0 5.4456709910740004E-5 7 0.0 0.0 0.0 0.0 5.4456709910740004E-5 8 0.0 0.0 0.0 0.0 5.4456709910740004E-5 9 0.0 0.0 0.0 0.0 5.4456709910740004E-5 10 0.0 0.0 0.0 0.0 5.4456709910740004E-5 11 0.0 0.0 0.0 0.0 5.4456709910740004E-5 12 0.0 0.0 0.0 0.0 5.4456709910740004E-5 13 0.0 0.0 0.0 0.0 5.4456709910740004E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1535 0.0 13.726638 1 TAGGACC 820 0.0 13.323304 4 CCAACGA 85 3.954792E-6 12.291228 19 TGAACCG 55 0.0030685805 12.09103 5 GTGGTAT 445 0.0 11.783734 1 GGCGAGG 1020 0.0 11.6394205 19 TGGCGAA 100 1.9354811E-6 11.397322 18 CTCGAAC 135 4.7457434E-9 11.256613 18 GGCGAAA 105 3.4765017E-6 10.854591 19 GCCGGTT 70 0.0014948935 10.854295 11 ACTGTTC 460 0.0 10.736315 8 AGGACCT 1660 0.0 10.587456 5 TGTAGGA 2380 0.0 10.53792 2 TGGCGAG 2400 0.0 10.487118 18 GGACCTG 1635 0.0 10.455974 6 TAGTACC 100 2.3987568E-5 10.450105 4 TTAGGAC 1475 0.0 10.3695965 3 TGGTATC 450 0.0 10.344548 2 GGACGTG 2215 0.0 10.333632 6 AGGACGT 2255 0.0 10.237353 5 >>END_MODULE