FastQCFastQC Report
Thu 26 May 2016
SRR1511857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761125
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62470.35471644545390024No Hit
TATCAACGCAGAGTACTTTTTTTTT41370.23490666477393712No Hit
GTCCTACAGTGGACATTTCTAAATT40940.23246504365107531No Hit
GGTATCAACGCAGAGTACTTTTTTT40900.23223791610476255No Hit
CTGTAGGACGTGGAATATGGCAAGA39360.22349350557172265No Hit
GTCCTAAAGTGTGTATTTCTCATTT37540.21315920221449358No Hit
CTTTAGGACGTGAAATATGGCGAGG34890.19811200227127546No Hit
CTGAAGGACCTGGAATATGGCGAGA18650.1058982184683086No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18400.10447867130385408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.013.6486161
GTCAACG602.6829058E-413.3157232
GGCGAGG11700.011.4644619
ACCGTGA600.00613176711.0201028
TAGGACC7350.010.9237164
ATAGGAC2900.010.748673
GCTCTAT1155.659509E-610.4210011
TAGAAAT7000.010.3903814
TGGCGAG25450.010.16983418
GAAATGT6750.010.0755216
TAGGACT3000.010.0755214
TGACGAG856.948063E-410.00171918
TTAGGAC16150.09.9429483
GGACGTG25950.09.93720156
AGGACGT26100.09.880095
TAGGACA7850.09.8669524
GTAGCAC1356.966038E-79.7956463
AAATGTC6950.09.7855787
GTATCAA21900.09.7131811
GACGTGG13250.09.69531257
Produced by FastQC (version 0.11.5)