##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511857_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1761125 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.919334516289304 31.0 30.0 34.0 25.0 34.0 2 27.137741500461352 31.0 27.0 34.0 10.0 34.0 3 28.907230321527432 31.0 28.0 34.0 16.0 34.0 4 33.34587153098162 35.0 33.0 37.0 28.0 37.0 5 33.73039733125133 35.0 35.0 37.0 30.0 37.0 6 33.94689701185322 37.0 35.0 37.0 30.0 37.0 7 33.94690212222301 37.0 35.0 37.0 30.0 37.0 8 33.971147987791895 37.0 35.0 37.0 30.0 37.0 9 35.56765192703528 39.0 35.0 39.0 30.0 39.0 10 35.4482140677124 39.0 35.0 39.0 30.0 39.0 11 35.44896699552843 39.0 35.0 39.0 30.0 39.0 12 35.34888835261552 39.0 35.0 39.0 30.0 39.0 13 35.34159188019022 39.0 35.0 39.0 30.0 39.0 14 36.46839378238342 40.0 37.0 41.0 30.0 41.0 15 36.4194216764852 40.0 36.0 41.0 29.0 41.0 16 36.370574490737454 40.0 36.0 41.0 29.0 41.0 17 36.3402563702179 40.0 36.0 41.0 29.0 41.0 18 36.31648065866988 40.0 36.0 41.0 29.0 41.0 19 36.35534161402513 40.0 37.0 41.0 29.0 41.0 20 36.30120235644829 40.0 37.0 41.0 29.0 41.0 21 36.2025852792959 40.0 36.0 41.0 29.0 41.0 22 36.11513208886365 40.0 36.0 41.0 29.0 41.0 23 36.00990730357016 40.0 36.0 41.0 28.0 41.0 24 35.91391298175882 40.0 36.0 41.0 28.0 41.0 25 35.81650081623962 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7085246598800587 1101 2 3.3356705714888584 1101 3 1.9865640903406359 1101 4 0.9838472849728817 1101 5 0.3385769808724106 1101 6 0.19976402745228228 1101 7 -0.08866525105064227 1101 8 0.04788654194181419 1101 9 0.09300134901250345 1101 10 0.013296022329242874 1101 11 0.09089702145864464 1101 12 0.19346305607965064 1101 13 0.07763885346990662 1101 14 -0.11915682349521006 1101 15 0.18526999009817047 1101 16 0.14258566628081581 1101 17 0.20497315069675892 1101 18 0.1299716422581696 1101 19 0.16733020489329675 1101 20 0.0754909525636478 1101 21 0.13490669273639355 1101 22 0.11030788692321636 1101 23 -0.1156256700736904 1101 24 0.02215454527453886 1101 25 0.053336459863686514 1102 1 1.1835397783574138 1102 2 3.8500982727363002 1102 3 2.210214539698029 1102 4 1.2285197624763953 1102 5 0.911473604825801 1102 6 0.8332494040216005 1102 7 0.7040942859901094 1102 8 0.6260312329239497 1102 9 0.5366574069911465 1102 10 0.6940822976133489 1102 11 0.7607209221974927 1102 12 0.7756432835912719 1102 13 0.719465051072703 1102 14 0.8122066247003943 1102 15 0.7369347395320673 1102 16 0.7496559629082 1102 17 0.7902793260939873 1102 18 0.7919560640264862 1102 19 0.9506027781630664 1102 20 0.9377348207572282 1102 21 0.7999106130481906 1102 22 0.8734195652554391 1102 23 1.08491153664216 1102 24 0.6256452443109666 1102 25 0.7951090512565528 1103 1 1.2143281738399025 1103 2 3.8038856755578294 1103 3 2.222621717222392 1103 4 1.4772265197284966 1103 5 0.9827217910325174 1103 6 0.8978974431727877 1103 7 0.840123179437029 1103 8 0.6359455517510355 1103 9 0.7690141778715542 1103 10 0.9358469350245926 1103 11 0.871154996279067 1103 12 0.74267725168297 1103 13 0.8439800172622185 1103 14 1.0295733474949387 1103 15 0.9075524088971605 1103 16 0.891358134254638 1103 17 0.9350212956981778 1103 18 1.0518983985944388 1103 19 1.0938879292420154 1103 20 1.1121948037959584 1103 21 1.1178227571307957 1103 22 1.3046779744963644 1103 23 1.3654098336466092 1103 24 1.241616674762156 1103 25 1.2189420239399382 1104 1 0.9995828591909586 1104 2 3.586398750875272 1104 3 1.892957538377356 1104 4 1.0364336152349836 1104 5 0.9132706831939998 1104 6 0.6337996713654093 1104 7 0.5137366951642619 1104 8 0.7045678492522285 1104 9 0.7817654805361229 1104 10 0.8159489510083944 1104 11 0.6990176251375146 1104 12 0.6906262937534393 1104 13 0.6403492439237723 1104 14 0.9877385894700126 1104 15 0.9114767424071033 1104 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-0.25916836056408243 2313 24 -0.3589133561353748 2313 25 -0.25685490640244524 2314 1 0.19772767650520962 2314 2 2.221167109736694 2314 3 1.170959360631997 2314 4 0.5394962776623089 2314 5 0.15403312680914638 2314 6 0.07196044520991762 2314 7 0.016014513462813795 2314 8 -0.1507228263274385 2314 9 -0.020462650637888657 2314 10 -0.26595750029232335 2314 11 -0.0258083519049066 2314 12 -0.2607700206657384 2314 13 -0.3893674332468251 2314 14 -0.9467163038265909 2314 15 -0.6495139079159244 2314 16 -0.7505182986584131 2314 17 -0.513557680435909 2314 18 -0.3245617235388494 2314 19 -0.3288069291879552 2314 20 -0.325806346083489 2314 21 -0.2540319321769786 2314 22 -0.2551713581713315 2314 23 -0.22604994541564594 2314 24 -0.0705554853786694 2314 25 -0.26465622332199956 2315 1 -4.126830301485011 2315 2 -13.677672361754121 2315 3 -6.766398234719837 2315 4 -2.9079918506587816 2315 5 -1.6611566686932377 2315 6 -1.192724513832033 2315 7 -0.8788648727012571 2315 8 -0.8494480713794772 2315 9 -0.8595986265171405 2315 10 -1.0162349938494302 2315 11 -0.9697894661979447 2315 12 -0.9763179342169153 2315 13 -0.8885722880722895 2315 14 -0.9379924109249771 2315 15 -1.0796395166358792 2315 16 -0.9331453856622289 2315 17 -0.8648963093553803 2315 18 -0.8371044323199541 2315 19 -0.91825016611498 2315 20 -0.690408662498804 2315 21 -0.9948680858539234 2315 22 -0.8398704643393557 2315 23 -1.0029829655344358 2315 24 -0.9987845517692548 2315 25 -1.1283841827655579 2316 1 -1.3382817139389225 2316 2 -1.2667471170742175 2316 3 -0.7952842148145862 2316 4 -0.919648912101195 2316 5 -1.0172406322078658 2316 6 -1.2311138759169324 2316 7 -1.03640140667315 2316 8 -1.1558604115595017 2316 9 -1.032123549437543 2316 10 -1.3453441997693076 2316 11 -1.3017750697348163 2316 12 -1.2282110629145109 2316 13 -1.0515953798998865 2316 14 -1.5188091548263358 2316 15 -2.117533108534822 2316 16 -1.6379656780167196 2316 17 -1.5373979688155401 2316 18 -1.4485694926705364 2316 19 -1.4526490693203726 2316 20 -1.5272852793092895 2316 21 -1.3702757133738714 2316 22 -1.4296167582116581 2316 23 -1.3695546138901733 2316 24 -1.3929487318305007 2316 25 -1.557236249904939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50490.0 3 1.0 4 489.0 5 2293.0 6 2841.0 7 2565.0 8 2273.0 9 2318.0 10 2193.0 11 2172.0 12 2139.0 13 2071.0 14 2156.0 15 2082.0 16 2285.0 17 2536.0 18 2868.0 19 3479.0 20 4565.0 21 5888.0 22 7158.0 23 8654.0 24 10016.0 25 11792.0 26 13396.0 27 15377.0 28 18139.0 29 22139.0 30 28322.0 31 36192.0 32 48320.0 33 65523.0 34 90804.0 35 133407.0 36 208767.0 37 284412.0 38 661003.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83320320817659 19.998637234722125 13.343800127759245 24.824359429342042 2 15.554599041524916 21.619478307642527 40.919730263043355 21.906192387789197 3 17.989637305699482 25.77943076158705 30.497551281141316 25.733380651572148 4 11.758336290723259 17.60681382638938 39.84191922776634 30.792930655121015 5 12.400255065393157 38.51901946321568 36.47162567847565 12.609099792915519 6 29.939106517033593 38.38140534254572 19.000651862350292 12.678836278070396 7 26.57818156008233 32.265001064660375 23.55324011640287 17.603577258854425 8 23.76702391936972 38.09002768116971 21.452196749236993 16.69075165022358 9 25.91264106749947 15.283071900063879 21.57688977216268 37.22739726027397 10 14.618496699552844 29.455830789978 35.5101994463766 20.415473064092556 11 32.569749449925474 22.754205408474697 26.379558520831853 18.296486620767975 12 22.87049471218681 25.16544822201718 33.00123500603308 18.962822059762935 13 28.270468178277053 23.029497071188626 26.5566195225322 22.143415228002116 14 19.868947405777558 23.535808077223365 28.816750656540563 27.778493860458514 15 21.769777840868763 32.796479523032154 24.139456313436014 21.29428632266307 16 20.60177443395557 28.91089502448719 29.65002484207538 20.837305699481863 17 20.492552153098636 29.709961825277535 29.00387425451973 20.793611767104096 18 20.15081269075165 27.842998083611327 31.991709844559583 20.014479381077436 19 22.570876811845196 27.340685098508395 29.002696580980615 21.085741508665798 20 22.63218322927053 27.978622646094863 29.836194776493997 19.55299934814061 21 21.751326293459073 27.27039919687061 28.667812534246846 22.31046197542347 22 22.11492597656871 29.07128872090963 28.797590547532625 20.01619475498903 23 20.833856201653607 28.776590338301954 29.577388012962214 20.812165447082222 24 22.084864342768952 27.397701313899947 30.259503615084494 20.257930728246606 25 21.46446192204807 28.234109036852306 29.709882000623473 20.591547040476154 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 239.0 2 503.5 3 768.0 4 768.0 5 768.0 6 1957.5 7 3147.0 8 3147.0 9 3147.0 10 4427.0 11 5707.0 12 5707.0 13 5707.0 14 8716.0 15 11725.0 16 11725.0 17 11725.0 18 20006.0 19 28287.0 20 28287.0 21 28287.0 22 42953.0 23 57619.0 24 57619.0 25 57619.0 26 81390.5 27 105162.0 28 105162.0 29 105162.0 30 133385.5 31 161609.0 32 161609.0 33 161609.0 34 178480.0 35 195351.0 36 195351.0 37 195351.0 38 203698.5 39 212046.0 40 212046.0 41 212046.0 42 217154.5 43 222263.0 44 222263.0 45 222263.0 46 222000.0 47 221737.0 48 221737.0 49 221737.0 50 207839.5 51 193942.0 52 193942.0 53 193942.0 54 167378.0 55 140814.0 56 140814.0 57 140814.0 58 118024.0 59 95234.0 60 95234.0 61 95234.0 62 75920.0 63 56606.0 64 56606.0 65 56606.0 66 42813.0 67 29020.0 68 29020.0 69 29020.0 70 20824.0 71 12628.0 72 12628.0 73 12628.0 74 8770.5 75 4913.0 76 4913.0 77 4913.0 78 3261.5 79 1610.0 80 1610.0 81 1610.0 82 1049.5 83 489.0 84 489.0 85 489.0 86 316.5 87 144.0 88 144.0 89 144.0 90 94.0 91 44.0 92 44.0 93 44.0 94 29.5 95 15.0 96 15.0 97 15.0 98 10.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.414380012775925 3 0.0 4 0.0 5 4.542550926254524E-4 6 9.652920718290865E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 5.678188657818155E-5 14 0.0 15 0.0 16 0.0 17 6.813826389381787E-4 18 0.0 19 3.406913194690894E-4 20 5.110369792036341E-4 21 7.949464120945418E-4 22 0.0034636950812690754 23 3.406913194690894E-4 24 0.0018170203705018097 25 7.949464120945418E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1761125.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.58294664802798 #Duplication Level Percentage of deduplicated Percentage of total 1 87.94661681568668 67.35211063470294 2 8.862412861320456 13.574193826626027 3 1.775936988551692 4.080194629335413 4 0.5295213434363516 1.6220921917351288 5 0.2566210113185136 0.9826396609284357 6 0.13840934124857643 0.6359877117857056 7 0.09519003679432642 0.5102953456470605 8 0.0630420954000231 0.38623595428798874 9 0.05419270262951305 0.37352131687695994 >10 0.24145819967234675 3.3198982182023724 >50 0.018316053057956725 0.9831022044528619 >100 0.014761450470476663 2.2986864545557184 >500 0.0021725938719966056 1.2160349180139223 >1k 0.001273589511170424 2.3065936215576186 >5k 7.491703006884845E-5 0.35841331129195436 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6247 0.35471644545390024 No Hit TATCAACGCAGAGTACTTTTTTTTT 4137 0.23490666477393712 No Hit GTCCTACAGTGGACATTTCTAAATT 4094 0.23246504365107531 No Hit GGTATCAACGCAGAGTACTTTTTTT 4090 0.23223791610476255 No Hit CTGTAGGACGTGGAATATGGCAAGA 3936 0.22349350557172265 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3754 0.21315920221449358 No Hit CTTTAGGACGTGAAATATGGCGAGG 3489 0.19811200227127546 No Hit CTGAAGGACCTGGAATATGGCGAGA 1865 0.1058982184683086 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1840 0.10447867130385408 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1356377315636312E-4 0.0 10 0.0 0.0 0.0 1.1356377315636312E-4 0.0 11 0.0 0.0 0.0 1.1356377315636312E-4 0.0 12 0.0 0.0 0.0 1.1356377315636312E-4 0.0 13 0.0 0.0 0.0 1.1356377315636312E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1200 0.0 13.648616 1 GTCAACG 60 2.6829058E-4 13.315723 2 GGCGAGG 1170 0.0 11.46446 19 ACCGTGA 60 0.006131767 11.020102 8 TAGGACC 735 0.0 10.923716 4 ATAGGAC 290 0.0 10.74867 3 GCTCTAT 115 5.659509E-6 10.421001 1 TAGAAAT 700 0.0 10.390381 4 TGGCGAG 2545 0.0 10.169834 18 GAAATGT 675 0.0 10.075521 6 TAGGACT 300 0.0 10.075521 4 TGACGAG 85 6.948063E-4 10.001719 18 TTAGGAC 1615 0.0 9.942948 3 GGACGTG 2595 0.0 9.9372015 6 AGGACGT 2610 0.0 9.88009 5 TAGGACA 785 0.0 9.866952 4 GTAGCAC 135 6.966038E-7 9.795646 3 AAATGTC 695 0.0 9.785578 7 GTATCAA 2190 0.0 9.713181 1 GACGTGG 1325 0.0 9.6953125 7 >>END_MODULE