FastQCFastQC Report
Thu 26 May 2016
SRR1511857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761125
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79250.44999645113208886No Hit
TATCAACGCAGAGTACTTTTTTTTT56030.3181489104975513No Hit
GGTATCAACGCAGAGTACTTTTTTT55860.3171836184257222No Hit
GTCCTACAGTGGACATTTCTAAATT41560.23598552061892256No Hit
CTGTAGGACGTGGAATATGGCAAGA40320.22894456668322805No Hit
CTTTAGGACGTGAAATATGGCGAGG38220.21702037050180994No Hit
GTCCTAAAGTGTGTATTTCTCATTT37090.2106040173184754No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25270.1434878273830648No Hit
CTGAAGGACCTGGAATATGGCGAGA20850.11839023351550855No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20290.11521044786713039No Hit
GTCCTACAGTGTGCATTTCTCATTT17780.1009581943360068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAA602.5741881E-514.24674119
GGTATCA13450.013.9656391
TAGGACC5500.012.9541584
TGGCGAA751.4823281E-512.66377118
TCCAACG658.0397597E-411.68963418
AGTCGTC2150.011.04367358
CCACCTT7750.011.02942313
ACCTTTT7950.010.99088615
TCGAACT1053.4759705E-610.85466119
GGCGAGG10700.010.74053519
TAGACTG2300.010.738815
ATTAGAC1701.382432E-1010.617333
AAGTCGT2250.010.5528427
TCCACCT8350.010.464375512
CTAGACT2001.8189894E-1210.4496884
TTCCACC7850.010.40496911
CACCTTT8500.010.39144614
CAAGTCG2200.010.3609726
TCGTCAA2400.010.289021510
GACCGAG750.002653522610.1307297
Produced by FastQC (version 0.11.5)