##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511857_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1761125 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.533321030591242 31.0 31.0 34.0 27.0 34.0 2 30.741819575555397 31.0 31.0 34.0 27.0 34.0 3 30.84122876002555 31.0 31.0 34.0 27.0 34.0 4 34.43983987507985 37.0 35.0 37.0 31.0 37.0 5 34.03283866846476 37.0 35.0 37.0 30.0 37.0 6 33.963672652423874 37.0 35.0 37.0 30.0 37.0 7 33.78969068067286 37.0 35.0 37.0 29.0 37.0 8 33.686219320036905 37.0 35.0 37.0 29.0 37.0 9 35.14339640854568 39.0 35.0 39.0 29.0 39.0 10 34.927102846192064 38.0 35.0 39.0 28.0 39.0 11 34.90723202498403 38.0 35.0 39.0 28.0 39.0 12 34.72738902690042 38.0 35.0 39.0 27.0 39.0 13 34.71156902548087 38.0 35.0 39.0 27.0 39.0 14 35.653331251330826 39.0 36.0 41.0 27.0 41.0 15 35.588874724962736 39.0 36.0 41.0 27.0 41.0 16 35.46937213428916 39.0 35.0 41.0 27.0 41.0 17 35.42505387181489 39.0 35.0 41.0 27.0 41.0 18 35.338475122435945 39.0 35.0 41.0 27.0 41.0 19 35.37019859464831 39.0 35.0 41.0 27.0 41.0 20 35.23308112712045 39.0 35.0 40.0 26.0 41.0 21 35.10621733267088 39.0 35.0 40.0 26.0 41.0 22 35.18302051245653 39.0 35.0 40.0 26.0 41.0 23 35.06535822272695 39.0 35.0 41.0 25.0 41.0 24 34.924200156150185 39.0 35.0 41.0 25.0 41.0 25 34.47129987933849 39.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5292852308081812 1101 2 0.45940150913078526 1101 3 0.4763596111305475 1101 4 0.3915231440558813 1101 5 0.4521739249412775 1101 6 0.5389472886278313 1101 7 0.29696063096528036 1101 8 0.3397821003833599 1101 9 0.47185196649824235 1101 10 0.628480333775812 1101 11 0.3595016416864141 1101 12 0.31581031286538774 1101 13 0.6011944505563278 1101 14 0.5590609505074084 1101 15 0.55517549277981 1101 16 0.5128216407424873 1101 17 0.5062878530766426 1101 18 0.4945564049539115 1101 19 0.6361253404208895 1101 20 0.5033092747200882 1101 21 0.44000674854909505 1101 22 0.5418404471690934 1101 23 0.41780622431846837 1101 24 0.4291142208583807 1101 25 0.6100480295896418 1102 1 0.63541713260771 1102 2 0.7193588149322743 1102 3 0.7092761132585821 1102 4 0.6846102322085272 1102 5 0.7311101182899122 1102 6 0.7935950996440013 1102 7 0.7420249488787221 1102 8 0.852556212208583 1102 9 0.8262409033974407 1102 10 0.9079930323429437 1102 11 0.8802819649642899 1102 12 1.0166815568537615 1102 13 1.050900570248885 1102 14 1.0992058971205836 1102 15 1.2524469176176112 1102 16 1.1870870353585943 1102 17 1.2952659584691233 1102 18 1.2938062995538004 1102 19 1.0720613410923931 1102 20 1.1396264327134205 1102 21 1.168838686404051 1102 22 0.9528695910149665 1102 23 1.1906898759505964 1102 24 0.972195872082267 1102 25 1.0503557025268933 1103 1 0.5844566807120017 1103 2 0.5782751588455568 1103 3 0.5485763776548751 1103 4 0.551191682823827 1103 5 0.6575574114632516 1103 6 0.6321069468682055 1103 7 0.6994381308089643 1103 8 0.6784996558383796 1103 9 0.9050790056726044 1103 10 0.8335430988215862 1103 11 0.8853417885121218 1103 12 0.8967582802194727 1103 13 0.7371983786588103 1103 14 1.0076620237473932 1103 15 1.0362713156768208 1103 16 0.8068364336527765 1103 17 0.9149510109326613 1103 18 0.9798528700294753 1103 19 0.9209252756881128 1103 20 0.8429834776058485 1103 21 0.8476481583995152 1103 22 1.1048515510468064 1103 23 1.1673337854008352 1103 24 1.2942655521099695 1103 25 1.0506365829224578 1104 1 0.34264013982703645 1104 2 0.3566010732138736 1104 3 0.40684077093372295 1104 4 0.4115433987810704 1104 5 0.5151000722503696 1104 6 0.6353234541001314 1104 7 0.7211881443675239 1104 8 0.7293894449228375 1104 9 0.6704825709476765 1104 10 0.7433279279768001 1104 11 0.8097663841541873 1104 12 0.8981259563381911 1104 13 0.9227580272766005 1104 14 0.865267262496225 1104 15 0.9257356900432185 1104 16 1.0799176195190583 1104 17 1.024151640049773 1104 18 0.9730874970578753 1104 19 1.149253484581692 1104 20 1.0659007787435115 1104 21 1.2345544847533176 1104 22 1.274836935616193 1104 23 1.2738567730674148 1104 24 1.3272142395719868 1104 25 1.4014430737867727 1105 1 0.47781803287278635 1105 2 0.5719354557987479 1105 3 0.6020597358491209 1105 4 0.5644003637495416 1105 5 0.6397895325798899 1105 6 0.6376066989411413 1105 7 0.5774662001085034 1105 8 0.8490949604340017 1105 9 0.8194701656916195 1105 10 0.828075403342865 1105 11 0.8802724132678605 1105 12 0.9407972201147174 1105 13 0.9799728564262011 1105 14 0.9072730476691149 1105 15 1.036919609140618 1105 16 0.7195456455584619 1105 17 0.9325187066160083 1105 18 1.0158087737969481 1105 19 0.9128299625668035 1105 20 1.0934170941967025 1105 21 1.042734359945463 1105 22 0.9970384041600866 1105 23 0.9498891324748513 1105 24 1.05986841728744 1105 25 1.3114350512993056 1106 1 0.12067684719684024 1106 2 0.25484099806962135 1106 3 0.3343429700958076 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0.6951324156250962 1107 17 0.8644737644935461 1107 18 0.7280196873261531 1107 19 0.8076366477433368 1107 20 0.9603857023156408 1107 21 0.8546035377541941 1107 22 0.6418885634348541 1107 23 0.8530838762447033 1107 24 0.5168909332881881 1107 25 0.5903143899980279 1108 1 0.22534838619302633 1108 2 0.13999408329326357 1108 3 0.23830050921836587 1108 4 0.2060192331286146 1108 5 0.20396766434756586 1108 6 0.3280744443029562 1108 7 0.40749914154733347 1108 8 0.36385516656070394 1108 9 0.5543333967209776 1108 10 0.42378100088826187 1108 11 0.6376092608673218 1108 12 0.5162996323277582 1108 13 0.6810074309991023 1108 14 0.9256172092869122 1108 15 0.9468155941684628 1108 16 0.7593339437058759 1108 17 0.7574455808512255 1108 18 0.6928106126347799 1108 19 0.8125561074187786 1108 20 0.7324704339510717 1108 21 0.9137042724060151 1108 22 0.7924881216057855 1108 23 1.0039238579612615 1108 24 1.012680551117917 1108 25 0.9765887379624942 1109 1 -0.07180283734013315 1109 2 -0.08227143355609456 1109 3 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-1.2193277340371083 2315 12 -1.101464818662791 2315 13 -1.1999311859082695 2315 14 -1.4155002300932438 2315 15 -1.464399852393413 2315 16 -1.578105503472628 2315 17 -1.6923728654107109 2315 18 -1.7198425183503403 2315 19 -1.5694096663084594 2315 20 -1.4760038611562791 2315 21 -1.5810594061223853 2315 22 -1.5797565260358652 2315 23 -1.4933155533589115 2315 24 -1.399530215429273 2315 25 -1.3089466151666471 2316 1 -1.7406895656121435 2316 2 -1.4551257318448627 2316 3 -1.2994001744471184 2316 4 -1.1114350781322244 2316 5 -1.4849876018583146 2316 6 -1.3397547270188568 2316 7 -1.4942724893530297 2316 8 -1.6130353911568136 2316 9 -1.8720726274618542 2316 10 -1.8478071917316186 2316 11 -1.7580190041393209 2316 12 -1.7916570472195659 2316 13 -1.9501229802137914 2316 14 -2.074437741337249 2316 15 -2.215157207265605 2316 16 -1.8451488314088351 2316 17 -2.082051333372277 2316 18 -2.1199740344455478 2316 19 -1.9689451208557927 2316 20 -2.0070616145057016 2316 21 -2.3762220538970595 2316 22 -2.3569892495563565 2316 23 -2.328237005577833 2316 24 -2.138421137716911 2316 25 -2.0972031170452965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34534.0 3 2.0 4 611.0 5 5498.0 6 6708.0 7 5441.0 8 4244.0 9 3828.0 10 3523.0 11 3323.0 12 3128.0 13 3108.0 14 3079.0 15 3048.0 16 3287.0 17 3253.0 18 3557.0 19 3957.0 20 4809.0 21 6127.0 22 7984.0 23 10001.0 24 12097.0 25 14126.0 26 16525.0 27 18905.0 28 22099.0 29 26776.0 30 33583.0 31 43084.0 32 55405.0 33 72458.0 34 94161.0 35 125982.0 36 185634.0 37 329149.0 38 588091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.70027744540448 21.06612686329358 14.203186717279884 24.03040897402206 2 14.641777273049897 21.875846404996807 41.27708141102988 22.205294910923413 3 18.193711406061468 26.417772730498974 31.08541415288523 24.303101710554333 4 12.502406132443749 18.587720917027468 39.163489246930226 29.74638370359855 5 12.365958442573415 38.877617786178725 36.388988599651945 12.367435171595913 6 29.95766910355597 39.003080417346865 18.516232521825536 12.523017957271632 7 26.527759244800908 32.5366456100504 23.48720278231244 17.448392362836255 8 23.972290439349848 38.305174249414435 21.203037830931933 16.519497480303784 9 25.966072822769537 15.236737880616083 21.393370714741998 37.403818581872386 10 14.672780183121583 29.873745475193413 35.28539995741358 20.168074384271417 11 32.74435375115338 22.81348569806232 26.182298246859254 18.259862303925047 12 22.834778905529134 25.480133437433462 32.86745688125488 18.817630775782526 13 28.435630633827806 22.981560082333736 26.54888210660799 22.033927177230463 14 19.93191851799276 23.64437504436085 28.866094115977003 27.55761232166939 15 21.8735183476471 32.80300943998864 24.168471857477464 21.15500035488679 16 20.67073111872368 29.124030519163057 29.45569389531566 20.749544466797605 17 20.4746965717936 29.967151678614524 28.948484633401943 20.609667116189936 18 20.245116604708024 27.980644146540754 31.91240124897721 19.861837999774007 19 22.589424373624816 27.484534033643264 29.09640144793811 20.82964014479381 20 22.722918588970117 27.978933920079495 29.80946837958691 19.488679111363474 21 21.79391014266449 27.340308041734684 28.77933139328554 22.08645042231528 22 22.219887855774008 29.041890836823054 28.927418553481438 19.8108027539215 23 20.842870324366526 28.8437220526652 29.641905032294698 20.671502590673573 24 22.112893196937044 27.496514853396704 30.305301681671963 20.08529026799429 25 21.569544869691025 28.26936464367602 29.782774844672883 20.378315641960068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 261.0 1 261.0 2 561.0 3 861.0 4 861.0 5 861.0 6 2112.5 7 3364.0 8 3364.0 9 3364.0 10 4705.0 11 6046.0 12 6046.0 13 6046.0 14 9069.5 15 12093.0 16 12093.0 17 12093.0 18 20395.5 19 28698.0 20 28698.0 21 28698.0 22 44202.5 23 59707.0 24 59707.0 25 59707.0 26 84035.5 27 108364.0 28 108364.0 29 108364.0 30 135605.5 31 162847.0 32 162847.0 33 162847.0 34 179880.5 35 196914.0 36 196914.0 37 196914.0 38 204433.5 39 211953.0 40 211953.0 41 211953.0 42 217084.5 43 222216.0 44 222216.0 45 222216.0 46 221056.0 47 219896.0 48 219896.0 49 219896.0 50 206322.0 51 192748.0 52 192748.0 53 192748.0 54 165832.0 55 138916.0 56 138916.0 57 138916.0 58 116123.5 59 93331.0 60 93331.0 61 93331.0 62 74307.5 63 55284.0 64 55284.0 65 55284.0 66 41833.5 67 28383.0 68 28383.0 69 28383.0 70 20337.5 71 12292.0 72 12292.0 73 12292.0 74 8552.5 75 4813.0 76 4813.0 77 4813.0 78 3163.0 79 1513.0 80 1513.0 81 1513.0 82 985.0 83 457.0 84 457.0 85 457.0 86 297.5 87 138.0 88 138.0 89 138.0 90 82.5 91 27.0 92 27.0 93 27.0 94 14.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3921357087089219 2 0.0 3 0.0 4 0.0 5 0.027084959897792606 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.135637731563631E-4 17 0.0 18 2.271275463127262E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004542550926254525 25 1.703456597345447E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1761125.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.18284998467468 #Duplication Level Percentage of deduplicated Percentage of total 1 87.46632685736986 65.75967730828161 2 9.176517978396024 13.798335491028372 3 1.8335569398949874 4.13556109051452 4 0.5945358976899645 1.7879561282611398 5 0.2735599637399428 1.028350885783658 6 0.13797628628434677 0.6224070259895143 7 0.09810097881074734 0.5162857821294717 8 0.07142700645861871 0.4296068729146177 9 0.04896547988657449 0.33132278958659417 >10 0.2598546494371635 3.494787937837481 >50 0.01907799457709225 1.0216141067019353 >100 0.01626118019769575 2.5494103016461143 >500 0.0021075694745007153 1.176627297051144 >1k 0.0015054067675005106 2.266388352231688 >5k 2.258110151250766E-4 1.0816686300421106 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7925 0.44999645113208886 No Hit TATCAACGCAGAGTACTTTTTTTTT 5603 0.3181489104975513 No Hit GGTATCAACGCAGAGTACTTTTTTT 5586 0.3171836184257222 No Hit GTCCTACAGTGGACATTTCTAAATT 4156 0.23598552061892256 No Hit CTGTAGGACGTGGAATATGGCAAGA 4032 0.22894456668322805 No Hit CTTTAGGACGTGAAATATGGCGAGG 3822 0.21702037050180994 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3709 0.2106040173184754 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2527 0.1434878273830648 No Hit CTGAAGGACCTGGAATATGGCGAGA 2085 0.11839023351550855 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2029 0.11521044786713039 No Hit GTCCTACAGTGTGCATTTCTCATTT 1778 0.1009581943360068 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.678188657818156E-5 0.0 10 0.0 0.0 0.0 5.678188657818156E-5 0.0 11 0.0 0.0 0.0 5.678188657818156E-5 0.0 12 0.0 0.0 0.0 5.678188657818156E-5 0.0 13 0.0 0.0 0.0 1.1356377315636312E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAAA 60 2.5741881E-5 14.246741 19 GGTATCA 1345 0.0 13.965639 1 TAGGACC 550 0.0 12.954158 4 TGGCGAA 75 1.4823281E-5 12.663771 18 TCCAACG 65 8.0397597E-4 11.689634 18 AGTCGTC 215 0.0 11.0436735 8 CCACCTT 775 0.0 11.029423 13 ACCTTTT 795 0.0 10.990886 15 TCGAACT 105 3.4759705E-6 10.854661 19 GGCGAGG 1070 0.0 10.740535 19 TAGACTG 230 0.0 10.73881 5 ATTAGAC 170 1.382432E-10 10.61733 3 AAGTCGT 225 0.0 10.552842 7 TCCACCT 835 0.0 10.4643755 12 CTAGACT 200 1.8189894E-12 10.449688 4 TTCCACC 785 0.0 10.404969 11 CACCTTT 850 0.0 10.391446 14 CAAGTCG 220 0.0 10.360972 6 TCGTCAA 240 0.0 10.2890215 10 GACCGAG 75 0.0026535226 10.130729 7 >>END_MODULE