FastQCFastQC Report
Thu 26 May 2016
SRR1511856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469078
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61870.42114850266629816No Hit
GTCCTACAGTGGACATTTCTAAATT58410.39759631551217844No Hit
CTGTAGGACGTGGAATATGGCAAGA53490.36410592221788085No Hit
GTCCTAAAGTGTGTATTTCTCATTT47010.31999662373270854No Hit
CTTTAGGACGTGAAATATGGCGAGG42390.28854832759050236No Hit
GGTATCAACGCAGAGTACTTTTTTT37700.2566235421128082No Hit
TATCAACGCAGAGTACTTTTTTTTT36210.2464811262574213No Hit
GTCCTACAGTGTGCATTTCTCATTT29360.1998532412846697No Hit
CTGAAGGACCTGGAATATGGCGAGA24260.1651375896991174No Hit
CTGTAGGACCTGGAATATGGCGAGA22970.15635657194512478No Hit
GTCCTTCAGTGTGCATTTCTCATTT17700.1204837319733874No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17420.1185777746314355No Hit
TTTCTAAATTTTCCACCTTTTTCAG16950.1153784890931591No Hit
ATTTAGAAATGTCCACTGTAGGACG16220.11040938602307024No Hit
GAATATGGCAAGAAAACTGAAAATC15700.10686975095944531No Hit
GGAATATGGCGAGAAAACTGAAAAT15330.10435116447186601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGA250.00622879818.89462517
CGATGTC250.00622879818.8946254
GTCAACG454.6767705E-415.5131642
GGTATCA9900.014.9087551
TGCCGGT1007.166818E-1014.17096910
GCCGGTT955.298716E-913.92235611
TAGGACC9900.013.4552644
CCGGTTT1001.0884833E-813.22623712
CGTTTCT1154.110916E-1013.14408814
CGGTAGG706.926551E-512.8221051
TAAGACG751.5654276E-512.5964174
AATACGG751.5654276E-512.59641714
ATACGGC701.1489328E-412.14654515
CGGTTTC1104.1196472E-812.02385313
ACGTTTC803.0339123E-511.80914113
GGCGAGG13500.011.68667619
TCCAACG1800.011.54671618
CGTTAGG709.937544E-411.3974271
GTGGTAT2650.011.2899051
CACCTTT7550.011.26169814
Produced by FastQC (version 0.11.5)