##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511856_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1469078 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.42179176326921 31.0 31.0 34.0 26.0 34.0 2 27.66413696209459 31.0 28.0 34.0 10.0 34.0 3 29.43296611888545 31.0 30.0 34.0 16.0 34.0 4 33.87624618978706 35.0 35.0 37.0 30.0 37.0 5 34.25481288263796 37.0 35.0 37.0 32.0 37.0 6 34.48747854096243 37.0 35.0 37.0 32.0 37.0 7 34.50748088256716 37.0 35.0 37.0 32.0 37.0 8 34.53131487912827 37.0 35.0 37.0 32.0 37.0 9 36.18853661956683 39.0 37.0 39.0 32.0 39.0 10 36.07828855921878 39.0 37.0 39.0 32.0 39.0 11 36.08667000663001 39.0 37.0 39.0 32.0 39.0 12 36.00223609638154 39.0 37.0 39.0 32.0 39.0 13 36.00260571596607 39.0 37.0 39.0 32.0 39.0 14 37.204750870954435 40.0 38.0 41.0 32.0 41.0 15 37.17501385222568 40.0 38.0 41.0 32.0 41.0 16 37.13763326385665 40.0 38.0 41.0 32.0 41.0 17 37.12406148618385 40.0 38.0 41.0 32.0 41.0 18 37.1095571508116 40.0 38.0 41.0 32.0 41.0 19 37.14842915080071 40.0 38.0 41.0 32.0 41.0 20 37.11081440195824 40.0 38.0 41.0 32.0 41.0 21 37.01449684768269 40.0 38.0 41.0 31.0 41.0 22 36.943701423613994 40.0 38.0 41.0 31.0 41.0 23 36.847673847134054 40.0 38.0 41.0 31.0 41.0 24 36.7801471399068 40.0 37.0 41.0 31.0 41.0 25 36.69860551992474 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.546899089935355 1101 2 3.33198382394016 1101 3 1.8182580743824914 1101 4 0.952424498374917 1101 5 0.32840206615136225 1101 6 0.3551916298011264 1101 7 0.1770818379746899 1101 8 0.1238913278637952 1101 9 0.024615841758716783 1101 10 0.03947689156133549 1101 11 0.10770277918383897 1101 12 0.08712548560765754 1101 13 0.0019486654069993392 1101 14 -0.1795811715946769 1101 15 -0.0029409271820171057 1101 16 -0.028170523399523972 1101 17 0.043458419976964535 1101 18 0.09499062186115026 1101 19 0.2568967955345016 1101 20 0.12555996230410216 1101 21 0.15118606903417486 1101 22 0.25183460646044153 1101 23 0.02625290636861166 1101 24 0.10885084644339571 1101 25 0.0015384774223008435 1102 1 1.1751198330344614 1102 2 4.026843455631383 1102 3 2.2207837423917667 1102 4 1.3140549645123798 1102 5 0.906317784624143 1102 6 0.8065835606265992 1102 7 0.7728120273997874 1102 8 0.780723487751537 1102 9 0.8091772865034983 1102 10 0.7951909839483662 1102 11 0.7918482650686585 1102 12 0.7582277409736307 1102 13 0.8118825322218157 1102 14 0.9297540421006261 1102 15 0.7384140374365487 1102 16 0.8646159023813809 1102 17 0.8862193825228601 1102 18 0.8944881236150906 1102 19 0.966138703389646 1102 20 1.0007384720460166 1102 21 0.9141500608147126 1102 22 0.9321766329065966 1102 23 0.9193669709775634 1102 24 0.7128841872554403 1102 25 0.7721645881001251 1103 1 0.8607331359407908 1103 2 3.5303915597684927 1103 3 1.9053605397437927 1103 4 1.0487592263490981 1103 5 0.6085734262981717 1103 6 0.3625994045991021 1103 7 0.38830615786585554 1103 8 0.2772165011901322 1103 9 0.32107739820624914 1103 10 0.5028330280401079 1103 11 0.5242430897364585 1103 12 0.38482875657775395 1103 13 0.5433282261836965 1103 14 0.6295357763029088 1103 15 0.4125401176295256 1103 16 0.400513259206555 1103 17 0.44303187952470324 1103 18 0.470906851300235 1103 19 0.5471488936954927 1103 20 0.6849434979843565 1103 21 0.7017332382439037 1103 22 0.5595211913380922 1103 23 0.7469335643178852 1103 24 0.8553223224363222 1103 25 0.7253659340154357 1104 1 1.0814486625533384 1104 2 3.8300083810273797 1104 3 2.159977140882326 1104 4 1.3106668909398635 1104 5 1.082339313061489 1104 6 0.80469420535632 1104 7 0.740618945503897 1104 8 0.7338113070270467 1104 9 0.7498515310167093 1104 10 0.8960289385425568 1104 11 0.878952649394158 1104 12 0.882348169499835 1104 13 0.9348029648331035 1104 14 0.9361727436539837 1104 15 0.9904974345994546 1104 16 1.058512263585257 1104 17 0.993925398842805 1104 18 1.0041932820656143 1104 19 1.013474712458546 1104 20 0.9677973075904092 1104 21 0.978546437337684 1104 22 0.925339209374421 1104 23 1.027098519125481 1104 24 0.9842747527343647 1104 25 1.015150994954908 1105 1 1.1875641054434887 1105 2 4.031822862312918 1105 3 2.2412378479830295 1105 4 1.4259948449251283 1105 5 1.0252395300139838 1105 6 0.9495297124093725 1105 7 0.6921695769816409 1105 8 0.7409272818115653 1105 9 0.925370799183753 1105 10 0.9368765801699226 1105 11 0.9456835796388106 1105 12 0.7325938979853746 1105 13 0.8748390061295837 1105 14 1.024469725279296 1105 15 0.9855635690361737 1105 16 0.9364047538072953 1105 17 0.8423002685957997 1105 18 0.9314881881283696 1105 19 0.9056897588608805 1105 20 0.9229931772712519 1105 21 0.9688641141841714 1105 22 1.0311434017758785 1105 23 0.9944239294140331 1105 24 0.8995542154036045 1105 25 1.1025897306235422 1106 1 0.6967044473724044 1106 2 3.5066123555966477 1106 3 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1107 16 1.0445880066536901 1107 17 1.0275926630879226 1107 18 0.8899310198237416 1107 19 1.0430718223518696 1107 20 1.116315075363275 1107 21 1.1165641405992375 1107 22 1.246670043743329 1107 23 1.1843643600473825 1107 24 1.1422739853893162 1107 25 1.2107458910916975 1108 1 1.2151320645152346 1108 2 3.9772600904452347 1108 3 2.314166001648193 1108 4 1.5407463489631112 1108 5 1.0189365956222787 1108 6 0.8565950276398198 1108 7 0.8773975585773215 1108 8 0.787695431792514 1108 9 0.7791490012042104 1108 10 0.7116359550260043 1108 11 0.9677063798916805 1108 12 0.9019986045671402 1108 13 0.7854319550488142 1108 14 0.9974221612556349 1108 15 0.9817784591168461 1108 16 1.0542509965383005 1108 17 0.8733368302205378 1108 18 1.015648585353489 1108 19 0.9723894997100047 1108 20 0.8608714893994289 1108 21 0.8950664025826711 1108 22 0.856361837911308 1108 23 1.0486932202449495 1108 24 1.094156006433387 1108 25 1.0752708745715935 1109 1 0.569260887983237 1109 2 2.7151763656305334 1109 3 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-0.9465493721463858 2315 8 -0.970711716314085 2315 9 -0.9640761564177325 2315 10 -0.8419219815126269 2315 11 -0.9115481231318228 2315 12 -0.9930296964681986 2315 13 -1.1054769014346348 2315 14 -1.1359150401007554 2315 15 -1.053559799499176 2315 16 -1.0955356175232538 2315 17 -0.970409221172595 2315 18 -1.076405205953776 2315 19 -1.0922890031072043 2315 20 -1.0545103489429906 2315 21 -1.225490850605226 2315 22 -1.2487375058141126 2315 23 -1.2374205769932871 2315 24 -1.3226299242774289 2315 25 -1.3458201657976687 2316 1 -1.2886373957799044 2316 2 -1.2717586689637699 2316 3 -0.8178570577310609 2316 4 -1.0022655232817996 2316 5 -1.251350790230184 2316 6 -1.0701427643209485 2316 7 -1.1542820440585473 2316 8 -1.2742209847186459 2316 9 -1.333260514620946 2316 10 -1.3901737284709412 2316 11 -1.4374815297097783 2316 12 -1.3904354310045193 2316 13 -1.1737183275592926 2316 14 -1.5052889835100345 2316 15 -1.665745226777986 2316 16 -1.4877115747898202 2316 17 -1.4225579151617254 2316 18 -1.4159634076872294 2316 19 -1.3195045605616968 2316 20 -1.3023416791467852 2316 21 -1.4697995951997385 2316 22 -1.3540423773215835 2316 23 -1.3328281759382676 2316 24 -1.340992869895146 2316 25 -1.5463236301205114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34611.0 3 0.0 4 334.0 5 1579.0 6 1891.0 7 1678.0 8 1551.0 9 1421.0 10 1360.0 11 1307.0 12 1333.0 13 1366.0 14 1355.0 15 1344.0 16 1444.0 17 1509.0 18 1722.0 19 2132.0 20 2867.0 21 3520.0 22 4343.0 23 5086.0 24 6141.0 25 7044.0 26 8014.0 27 9217.0 28 11093.0 29 13548.0 30 17474.0 31 23615.0 32 32427.0 33 46175.0 34 68208.0 35 106198.0 36 176547.0 37 251780.0 38 617844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.91743256654855 20.078784108127685 13.809273571587077 25.1945097537367 2 14.52515853198484 21.163777462410405 43.23681894120395 21.074245064400813 3 18.170035900067933 25.39409071540109 30.41118306856409 26.024690315966886 4 11.213972300994229 17.64269834549289 40.49744125226843 30.645888101244456 5 11.608039927355303 38.79051207976758 37.154304633958404 12.447143358918716 6 29.230689640854173 39.140571341441415 19.104208175381824 12.524530842322587 7 26.192959121299207 32.05990423925755 23.71977526040142 18.02736137904182 8 22.4341389633498 40.12060625780251 21.54085759911999 15.904397179727695 9 25.210914600858498 15.750491124365078 22.15280604569669 36.885788229079736 10 14.119263919274537 29.802093558000326 36.12109091552661 19.95755160719853 11 31.952217649437266 22.654413176155387 27.37533337236008 18.01803580204727 12 23.2027162614919 25.049452786033143 33.74225194305544 18.005579009419513 13 28.2405106890463 23.492962233352436 26.758080260082203 21.508446817519065 14 18.73249752565895 24.683236696758105 29.14739721103985 27.436868566543097 15 20.627767892514896 34.912101331583486 24.168151725095605 20.291979050806017 16 19.118794236929556 29.35419358264163 31.797903174644233 19.729109005784583 17 19.272111422797128 30.66060115542407 30.323517379350623 19.743770042428174 18 18.83650834060547 28.049565782075558 34.31465177478664 18.799274102532337 19 21.957490257474944 27.404999744737335 29.74361417899018 20.893895818797542 20 21.923393711667927 28.597343897840133 30.898595710211225 18.58066668028072 21 20.325089801650158 27.845829098797815 29.24017967817757 22.58890142137446 22 21.008932497763812 30.61344381076338 29.584457099913003 18.793166591559803 23 19.210945125977315 30.05212807523651 30.804642924726732 19.932283874059443 24 21.465962171265353 27.520111746294525 31.956744599541743 19.05718148289838 25 20.23297859450452 29.084681245583916 31.24966815559861 19.432672004312955 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 154.0 1 154.0 2 369.0 3 584.0 4 584.0 5 584.0 6 1588.0 7 2592.0 8 2592.0 9 2592.0 10 3792.0 11 4992.0 12 4992.0 13 4992.0 14 7654.5 15 10317.0 16 10317.0 17 10317.0 18 17513.5 19 24710.0 20 24710.0 21 24710.0 22 39020.0 23 53330.0 24 53330.0 25 53330.0 26 75989.5 27 98649.0 28 98649.0 29 98649.0 30 126422.5 31 154196.0 32 154196.0 33 154196.0 34 167548.0 35 180900.0 36 180900.0 37 180900.0 38 185338.5 39 189777.0 40 189777.0 41 189777.0 42 191120.5 43 192464.0 44 192464.0 45 192464.0 46 188929.5 47 185395.0 48 185395.0 49 185395.0 50 168561.5 51 151728.0 52 151728.0 53 151728.0 54 124950.5 55 98173.0 56 98173.0 57 98173.0 58 79562.5 59 60952.0 60 60952.0 61 60952.0 62 47467.5 63 33983.0 64 33983.0 65 33983.0 66 25020.5 67 16058.0 68 16058.0 69 16058.0 70 11333.5 71 6609.0 72 6609.0 73 6609.0 74 4492.0 75 2375.0 76 2375.0 77 2375.0 78 1577.0 79 779.0 80 779.0 81 779.0 82 516.5 83 254.0 84 254.0 85 254.0 86 161.5 87 69.0 88 69.0 89 69.0 90 48.0 91 27.0 92 27.0 93 27.0 94 17.5 95 8.0 96 8.0 97 8.0 98 5.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.268814862110793 3 0.0 4 0.0 5 6.806990506971038E-4 6 6.126291456273936E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.722796202788416E-4 14 0.0 15 0.0 16 0.0 17 4.7648933548797266E-4 18 0.0 19 2.0420971520913116E-4 20 5.445592405576832E-4 21 4.7648933548797266E-4 22 0.004084194304182624 23 2.722796202788416E-4 24 0.0024505165825095742 25 0.0010891184811153663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1469078.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.26732064262173 #Duplication Level Percentage of deduplicated Percentage of total 1 90.79704765127975 69.24847546623556 2 6.856965190510852 10.459247256399738 3 1.158176241893517 2.6499299640347846 4 0.3918884503502959 1.1955312839602465 5 0.190690935211493 0.7271743349708171 6 0.11304635933468275 0.517304576091557 7 0.08084430226038138 0.4316044825835075 8 0.05994952381468408 0.36577516441175983 9 0.04286515160576316 0.2942289235720197 >10 0.2582087913976179 3.6476901111874716 >50 0.024411030227678727 1.3307849548213107 >100 0.02068924550037806 3.2304913847083743 >500 0.0029683651418545773 1.6311002884969295 >1k 0.0019789100945697183 3.0785508683672487 >5k 2.698513765322343E-4 1.1921109401586112 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6187 0.42114850266629816 No Hit GTCCTACAGTGGACATTTCTAAATT 5841 0.39759631551217844 No Hit CTGTAGGACGTGGAATATGGCAAGA 5349 0.36410592221788085 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4701 0.31999662373270854 No Hit CTTTAGGACGTGAAATATGGCGAGG 4239 0.28854832759050236 No Hit GGTATCAACGCAGAGTACTTTTTTT 3770 0.2566235421128082 No Hit TATCAACGCAGAGTACTTTTTTTTT 3621 0.2464811262574213 No Hit GTCCTACAGTGTGCATTTCTCATTT 2936 0.1998532412846697 No Hit CTGAAGGACCTGGAATATGGCGAGA 2426 0.1651375896991174 No Hit CTGTAGGACCTGGAATATGGCGAGA 2297 0.15635657194512478 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1770 0.1204837319733874 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1742 0.1185777746314355 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1695 0.1153784890931591 No Hit ATTTAGAAATGTCCACTGTAGGACG 1622 0.11040938602307024 No Hit GAATATGGCAAGAAAACTGAAAATC 1570 0.10686975095944531 No Hit GGAATATGGCGAGAAAACTGAAAAT 1533 0.10435116447186601 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.0420971520913116E-4 0.0 5 0.0 6.80699050697104E-5 0.0 2.0420971520913116E-4 0.0 6 0.0 6.80699050697104E-5 0.0 2.0420971520913116E-4 0.0 7 0.0 6.80699050697104E-5 0.0 2.0420971520913116E-4 0.0 8 0.0 6.80699050697104E-5 0.0 2.0420971520913116E-4 0.0 9 0.0 6.80699050697104E-5 0.0 4.084194304182623E-4 0.0 10 0.0 6.80699050697104E-5 0.0 4.084194304182623E-4 0.0 11 0.0 6.80699050697104E-5 0.0 4.084194304182623E-4 0.0 12 0.0 6.80699050697104E-5 0.0 4.764893354879727E-4 0.0 13 0.0 6.80699050697104E-5 0.0 4.764893354879727E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGCGA 25 0.006228798 18.894625 17 CGATGTC 25 0.006228798 18.894625 4 GTCAACG 45 4.6767705E-4 15.513164 2 GGTATCA 990 0.0 14.908755 1 TGCCGGT 100 7.166818E-10 14.170969 10 GCCGGTT 95 5.298716E-9 13.922356 11 TAGGACC 990 0.0 13.455264 4 CCGGTTT 100 1.0884833E-8 13.226237 12 CGTTTCT 115 4.110916E-10 13.144088 14 CGGTAGG 70 6.926551E-5 12.822105 1 TAAGACG 75 1.5654276E-5 12.596417 4 AATACGG 75 1.5654276E-5 12.596417 14 ATACGGC 70 1.1489328E-4 12.146545 15 CGGTTTC 110 4.1196472E-8 12.023853 13 ACGTTTC 80 3.0339123E-5 11.809141 13 GGCGAGG 1350 0.0 11.686676 19 TCCAACG 180 0.0 11.546716 18 CGTTAGG 70 9.937544E-4 11.397427 1 GTGGTAT 265 0.0 11.289905 1 CACCTTT 755 0.0 11.261698 14 >>END_MODULE