FastQCFastQC Report
Thu 26 May 2016
SRR1511856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469078
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82660.562665835306226No Hit
GGTATCAACGCAGAGTACTTTTTTT58100.3954861484550174No Hit
TATCAACGCAGAGTACTTTTTTTTT57710.3928314221572987No Hit
CTGTAGGACGTGGAATATGGCAAGA52200.35532490446388826No Hit
GTCCTACAGTGGACATTTCTAAATT49160.3346316533226963No Hit
CTTTAGGACGTGAAATATGGCGAGG41270.2809244982226948No Hit
GTCCTAAAGTGTGTATTTCTCATTT40970.27888240107060347No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28770.1958371168855568No Hit
CTGAAGGACCTGGAATATGGCGAGA26650.18140629701077818No Hit
GTCCTACAGTGTGCATTTCTCATTT25290.1721487899212976No Hit
CTGTAGGACCTGGAATATGGCGAGA21980.14961765134322344No Hit
TTTCTAAATTTTCCACCTTTTTCAG19610.13348508384170207No Hit
GAATATGGCAAGAAAACTGAAAATC18290.1244998563725003No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17740.12075601159366622No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17480.11898619406185376No Hit
ATTTAGAAATGTCCACTGTAGGACG17440.11871391444157492No Hit
GGAATATGGCGAGAAAACTGAAAAT16310.11102201516869764No Hit
GTCCTTCAGTGTGCATTTCTCATTT15130.10298976637047183No Hit
ATTCCAGGTCCTTCAGTGTGCATTT14940.10169643817414732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8000.015.2007084
GGTATCA13700.014.4007711
ACCGTGC400.005288025414.2462998
CGGGGAA500.00150241713.2965459
CCGGTTT655.465396E-513.1504314
AGGACCT17150.012.1871575
GCCGGTT550.00307428412.08776913
TTAAGCG550.00307428412.08776912
TATGTCG1103.826608E-812.08776916
GGACCTG16300.011.7699476
TGTAGGA30150.011.6274072
CCACCTT6850.011.50795913
CTGTAGG29500.011.4048251
AAAGGCG752.07277E-411.4005315
CGGTTTC752.0786768E-411.39703815
GGACGGG752.0786768E-411.3970386
GTAGGAC30200.011.2935723
CGGCAAG600.005882296211.08158618
CACCTTT7050.011.04677514
GTGGTAT3850.010.89250851
Produced by FastQC (version 0.11.5)