##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511856_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1469078 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.173291002928366 31.0 30.0 33.0 25.0 34.0 2 29.400272143480468 31.0 30.0 34.0 25.0 34.0 3 29.4414108713084 31.0 30.0 34.0 25.0 34.0 4 33.00235794151161 35.0 33.0 37.0 27.0 37.0 5 32.48699660603453 35.0 33.0 37.0 25.0 37.0 6 32.44947375156391 35.0 33.0 37.0 25.0 37.0 7 32.23066031892112 35.0 32.0 37.0 23.0 37.0 8 32.083514966530025 35.0 32.0 37.0 22.0 37.0 9 33.313073233688065 37.0 33.0 39.0 22.0 39.0 10 33.00613990543729 37.0 32.0 39.0 19.0 39.0 11 33.014526798440926 37.0 32.0 39.0 19.0 39.0 12 32.74535116583326 37.0 32.0 39.0 17.0 39.0 13 32.73910847483932 37.0 32.0 39.0 17.0 39.0 14 33.37867968889331 38.0 32.0 40.0 17.0 41.0 15 33.316711570114045 38.0 32.0 40.0 17.0 41.0 16 33.15508706821557 38.0 32.0 40.0 16.0 41.0 17 33.08393563854336 38.0 32.0 40.0 13.0 41.0 18 32.969567987540486 38.0 32.0 40.0 11.0 41.0 19 33.00214079851444 38.0 32.0 40.0 10.0 41.0 20 32.83031534064222 38.0 32.0 40.0 10.0 41.0 21 32.642342339889375 38.0 32.0 40.0 10.0 41.0 22 32.7139477958284 38.0 32.0 40.0 2.0 41.0 23 32.54024224717816 38.0 32.0 40.0 2.0 41.0 24 32.328898805917724 38.0 32.0 40.0 2.0 41.0 25 31.79506057540852 37.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1096288639779317 1101 2 0.0025881466684616328 1101 3 0.14475142662363183 1101 4 0.2718510661858602 1101 5 0.11849627107729788 1101 6 0.2764757667928279 1101 7 0.04367659408256941 1101 8 -0.11669087786384225 1101 9 0.03422294376373003 1101 10 0.024946103563799227 1101 11 -0.1276098101140022 1101 12 -0.016638606967447345 1101 13 -0.030449329963154526 1101 14 -0.2196523239592949 1101 15 -0.3904530980463434 1101 16 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0.47716779245646634 1103 4 0.5125382072665943 1103 5 0.4512828485152909 1103 6 0.44566335602445406 1103 7 0.6495695125899559 1103 8 0.2783142767570723 1103 9 0.5414916677913482 1103 10 0.39726561212117417 1103 11 0.4476937102618024 1103 12 0.7310577557478126 1103 13 0.3502309382896982 1103 14 0.4299149358725671 1103 15 0.4796283130798429 1103 16 0.16237958514998496 1103 17 0.45134203457767796 1103 18 0.5082731253561903 1103 19 0.48885206570317763 1103 20 0.4940086488265649 1103 21 0.31518794128373173 1103 22 0.373229036994033 1103 23 0.4229977206098994 1103 24 0.5355121949936432 1103 25 0.5128207887411413 1104 1 0.45378269686514017 1104 2 0.3805365722096603 1104 3 0.4363320801300645 1104 4 0.49500308113763225 1104 5 0.6814728631786906 1104 6 0.6060218812858054 1104 7 0.777693105089611 1104 8 0.5478296394646236 1104 9 0.7878258172632329 1104 10 0.2995643459211621 1104 11 0.5260734889900505 1104 12 0.7670728322989007 1104 13 0.773385229112705 1104 14 0.9808186145916693 1104 15 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1.2492883723686816 1107 15 0.9017487319280093 1107 16 1.0622452776027913 1107 17 1.2398811238836558 1107 18 1.1363710844282693 1107 19 1.1941922431395113 1107 20 1.167455055672491 1107 21 1.0609861313784634 1107 22 1.198589924449088 1107 23 1.0994395616827148 1107 24 1.12929167088663 1107 25 0.9657533410629888 1108 1 0.08301216811040035 1108 2 0.18617418821711595 1108 3 0.16282522357005647 1108 4 0.24810395882768432 1108 5 0.18671255945007914 1108 6 0.37230070213377786 1108 7 0.5906934954539054 1108 8 0.3045734975231227 1108 9 0.29968029952684105 1108 10 0.049206092234740595 1108 11 0.2474478231670716 1108 12 0.39396767319396986 1108 13 0.5323377935542979 1108 14 0.7407508674973684 1108 15 0.5658742677724007 1108 16 0.5085070746511988 1108 17 0.4150086251967906 1108 18 0.47355826143906654 1108 19 0.5388719225647804 1108 20 0.37081456701629634 1108 21 0.6584670273611124 1108 22 0.6544638802938181 1108 23 0.716389188074217 1108 24 0.40625078574795026 1108 25 0.6717120153882057 1109 1 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-0.32291742508592236 2314 3 -0.37714585678524415 2314 4 -0.2925750503302069 2314 5 -0.4806775286859768 2314 6 -0.6278499210157129 2314 7 -0.49563422609122654 2314 8 -0.6029753043773098 2314 9 -0.4579580155595764 2314 10 -0.3891472954950643 2314 11 -0.4947348353745511 2314 12 -0.4886887166703815 2314 13 -0.5491114593548829 2314 14 -0.6066420609609366 2314 15 -0.7617280698988793 2314 16 -0.6694266339489516 2314 17 -0.5618134845601475 2314 18 -0.4012110699662941 2314 19 -0.6060159277416304 2314 20 -0.233263447168099 2314 21 -0.12160897277659899 2314 22 -0.2703480559452487 2314 23 -0.42525897346265396 2314 24 -0.7011465322992869 2314 25 -0.627095049267858 2315 1 -0.6796265972166395 2315 2 -0.5693448360096021 2315 3 -0.6660469898416714 2315 4 -0.8273676176495783 2315 5 -0.9700171559923554 2315 6 -0.9981011854614756 2315 7 -0.8611032012544051 2315 8 -0.8041166609368879 2315 9 -1.0632911157827962 2315 10 -1.2026653862526047 2315 11 -1.191244011436261 2315 12 -1.2931066831025255 2315 13 -1.145592556243919 2315 14 -1.2350804351238622 2315 15 -1.340092802458006 2315 16 -1.5431542412502424 2315 17 -1.6719845026155866 2315 18 -1.5665815031272636 2315 19 -1.7625166977113942 2315 20 -1.7585347025720175 2315 21 -1.7995081915045255 2315 22 -1.8591850837206714 2315 23 -1.6617165121024193 2315 24 -1.7012622043628056 2315 25 -1.6206280096250119 2316 1 -2.364441654536108 2316 2 -2.0227299954646334 2316 3 -1.8904511374110946 2316 4 -1.9732723922373943 2316 5 -2.1832219043156833 2316 6 -2.009921129455229 2316 7 -2.135276702236549 2316 8 -1.9252301642125502 2316 9 -2.2744788624747656 2316 10 -2.272587184667117 2316 11 -2.00444524549723 2316 12 -2.4598464094339683 2316 13 -2.317761817915894 2316 14 -2.640375688650149 2316 15 -2.6471126339953663 2316 16 -2.520158942963352 2316 17 -2.3590301917254415 2316 18 -2.4216653460286395 2316 19 -2.4636305339171756 2316 20 -2.4428808833086784 2316 21 -2.47665660805189 2316 22 -2.5274433413124058 2316 23 -2.33642066670037 2316 24 -2.1378297688310255 2316 25 -2.3026788221594607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54498.0 3 4.0 4 961.0 5 7106.0 6 8540.0 7 6912.0 8 5585.0 9 4960.0 10 4735.0 11 4406.0 12 4161.0 13 3964.0 14 3818.0 15 3891.0 16 4061.0 17 4196.0 18 4409.0 19 5124.0 20 6203.0 21 7698.0 22 9892.0 23 12136.0 24 14583.0 25 17163.0 26 19713.0 27 22410.0 28 26167.0 29 31340.0 30 38896.0 31 49484.0 32 62376.0 33 79737.0 34 103206.0 35 137148.0 36 191308.0 37 271449.0 38 236838.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.09805252958025 23.27216563437778 15.65060157838758 21.979180257654388 2 15.362628805277867 23.43660445531143 41.67457412063893 19.526192618771773 3 19.933046441373435 27.425705102111664 31.174791263636102 21.4664571928788 4 13.88163188067618 20.249231150422236 38.811213563881566 27.057923405020016 5 12.050124596592998 39.901344009150705 36.517355011778804 11.531176382477499 6 29.531719895063436 40.37260104637058 18.207270138141066 11.88840892042492 7 26.32481052741924 32.95291332386708 23.58860455333209 17.133671595381593 8 23.159968361108124 40.88925162584968 20.850152272377642 15.100627740664551 9 25.746897033377397 15.779556973829845 21.97881936833851 36.49472662445425 10 14.572201067608393 31.159543604900485 35.41541020966893 18.8528451178222 11 32.75047342618976 22.909947599787078 26.892785815320902 17.446793158702263 12 23.362204049070236 26.07839747106689 33.33342409320676 17.22597438665612 13 29.059791243215134 23.68539995834122 26.58354423658921 20.671264561854443 14 19.260719988999902 25.32540818118575 29.118127151859873 26.295744677954474 15 21.330657732264726 35.38185174646956 23.952029776499273 19.335460744766443 16 19.62305609368597 30.2720481826016 30.971806806718227 19.133088916994197 17 19.782748090979513 31.428555869735987 29.728101571189548 19.060594468094955 18 19.3809578265522 28.502950153702056 33.90988621419177 18.206205805553967 19 22.563403713077182 27.704315223561988 30.01256570447587 19.719715358884958 20 22.49546994781761 28.97497614149827 30.787541573694522 17.742012336989596 21 21.031898918913768 28.053105417139186 29.47243100774772 21.44256465619933 22 21.634249508875637 30.63520112614851 29.804271794962556 17.9262775700133 23 19.860824272094472 30.297165977572327 30.751736803627853 19.090272946705348 24 21.844215731456856 28.219875792464634 31.70743495714411 18.2284735189344 25 20.834048068309606 29.625220206442687 30.984646131309752 18.556085593937958 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 165.0 1 165.0 2 441.0 3 717.0 4 717.0 5 717.0 6 1885.0 7 3053.0 8 3053.0 9 3053.0 10 4480.0 11 5907.0 12 5907.0 13 5907.0 14 8732.5 15 11558.0 16 11558.0 17 11558.0 18 19364.5 19 27171.0 20 27171.0 21 27171.0 22 42777.5 23 58384.0 24 58384.0 25 58384.0 26 82304.5 27 106225.0 28 106225.0 29 106225.0 30 133307.0 31 160389.0 32 160389.0 33 160389.0 34 173547.0 35 186705.0 36 186705.0 37 186705.0 38 188659.0 39 190613.0 40 190613.0 41 190613.0 42 190539.0 43 190465.0 44 190465.0 45 190465.0 46 184391.5 47 178318.0 48 178318.0 49 178318.0 50 161739.0 51 145160.0 52 145160.0 53 145160.0 54 118798.0 55 92436.0 56 92436.0 57 92436.0 58 74539.0 59 56642.0 60 56642.0 61 56642.0 62 43636.0 63 30630.0 64 30630.0 65 30630.0 66 22842.0 67 15054.0 68 15054.0 69 15054.0 70 10639.0 71 6224.0 72 6224.0 73 6224.0 74 4242.5 75 2261.0 76 2261.0 77 2261.0 78 1503.0 79 745.0 80 745.0 81 745.0 82 461.0 83 177.0 84 177.0 85 177.0 86 116.5 87 56.0 88 56.0 89 56.0 90 37.0 91 18.0 92 18.0 93 18.0 94 9.5 95 1.0 96 1.0 97 1.0 98 2.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35253403835603014 2 0.0 3 0.0 4 0.0 5 0.02300762791356211 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.361398101394208E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005785941930925383 25 1.361398101394208E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1469078.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.80910454601386 #Duplication Level Percentage of deduplicated Percentage of total 1 91.38168208880141 69.27563491060549 2 6.351684826503357 9.630310781114458 3 1.0861682797121626 2.470243340137901 4 0.382419922947018 1.1596364767667622 5 0.18802994171267165 0.712719075453841 6 0.11285266623495953 0.5133155743741473 7 0.07872694249517574 0.41777533099433595 8 0.05661245320879896 0.3433391507129726 9 0.04721430580390967 0.3221346820280452 >10 0.2639874954631925 3.7772097415285066 >50 0.02292006672492592 1.2285218916206813 >100 0.022356902053572717 3.554419245593138 >500 0.002761122641734738 1.5910886741336001 >1k 0.0022267118078505947 3.3110768678275906 >5k 3.562738892560952E-4 1.692574257108596 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8266 0.562665835306226 No Hit GGTATCAACGCAGAGTACTTTTTTT 5810 0.3954861484550174 No Hit TATCAACGCAGAGTACTTTTTTTTT 5771 0.3928314221572987 No Hit CTGTAGGACGTGGAATATGGCAAGA 5220 0.35532490446388826 No Hit GTCCTACAGTGGACATTTCTAAATT 4916 0.3346316533226963 No Hit CTTTAGGACGTGAAATATGGCGAGG 4127 0.2809244982226948 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4097 0.27888240107060347 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2877 0.1958371168855568 No Hit CTGAAGGACCTGGAATATGGCGAGA 2665 0.18140629701077818 No Hit GTCCTACAGTGTGCATTTCTCATTT 2529 0.1721487899212976 No Hit CTGTAGGACCTGGAATATGGCGAGA 2198 0.14961765134322344 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1961 0.13348508384170207 No Hit GAATATGGCAAGAAAACTGAAAATC 1829 0.1244998563725003 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1774 0.12075601159366622 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1748 0.11898619406185376 No Hit ATTTAGAAATGTCCACTGTAGGACG 1744 0.11871391444157492 No Hit GGAATATGGCGAGAAAACTGAAAAT 1631 0.11102201516869764 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1513 0.10298976637047183 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1494 0.10169643817414732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.80699050697104E-5 0.0 10 0.0 0.0 0.0 6.80699050697104E-5 0.0 11 0.0 0.0 0.0 6.80699050697104E-5 0.0 12 0.0 0.0 0.0 6.80699050697104E-5 0.0 13 0.0 0.0 0.0 1.361398101394208E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 800 0.0 15.200708 4 GGTATCA 1370 0.0 14.400771 1 ACCGTGC 40 0.0052880254 14.246299 8 CGGGGAA 50 0.001502417 13.296545 9 CCGGTTT 65 5.465396E-5 13.15043 14 AGGACCT 1715 0.0 12.187157 5 GCCGGTT 55 0.003074284 12.087769 13 TTAAGCG 55 0.003074284 12.087769 12 TATGTCG 110 3.826608E-8 12.087769 16 GGACCTG 1630 0.0 11.769947 6 TGTAGGA 3015 0.0 11.627407 2 CCACCTT 685 0.0 11.507959 13 CTGTAGG 2950 0.0 11.404825 1 AAAGGCG 75 2.07277E-4 11.400531 5 CGGTTTC 75 2.0786768E-4 11.397038 15 GGACGGG 75 2.0786768E-4 11.397038 6 GTAGGAC 3020 0.0 11.293572 3 CGGCAAG 60 0.0058822962 11.081586 18 CACCTTT 705 0.0 11.046775 14 GTGGTAT 385 0.0 10.8925085 1 >>END_MODULE