FastQCFastQC Report
Thu 26 May 2016
SRR1511855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282900
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61460.2692189758640326No Hit
CTGTAGGACGTGGAATATGGCAAGA55860.24468877305182007No Hit
GTCCTAAAGTGTGTATTTCTCATTT55790.2443821455166674No Hit
CTTTAGGACGTGAAATATGGCGAGG54280.23776775154408866No Hit
GTATCAACGCAGAGTACTTTTTTTT52190.2286127294231022No Hit
GGTATCAACGCAGAGTACTTTTTTT34290.15020368829120856No Hit
TATCAACGCAGAGTACTTTTTTTTT32920.14420254938893512No Hit
GTCCTACAGTGTGCATTTCTCATTT29550.12944062376801438No Hit
CTGAAGGACCTGGAATATGGCGAGA28110.12313285733058829No Hit
CTGTAGGACCTGGAATATGGCGAGA22960.1005738315300714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.014.1334071
CGCTTAC450.00822393913.3275541
GCGCCAC604.2993322E-412.59313513
GGCGAAA854.208814E-612.22328419
TCCAACG2100.012.14391118
TAGGACC11850.011.8758044
GGCGAGG16800.011.80658119
GCCCGGA709.7521645E-411.4236181
GCCGGTT1105.3599433E-711.16209611
GGACGTG34400.011.009846
AGGACGT34800.010.9918525
AATGTCC8800.010.947448
TGGCGAA1053.7164318E-610.79458718
GTATCAA23250.010.791021
CCGGTTT1252.42846E-710.57823412
TGTCGAG1804.0017767E-1110.49473918
GACGTGA20350.010.4891227
TTAGGAC23000.010.4714653
GTCGAGG1451.5801561E-810.42236119
TGTAGGA34600.010.3712242
Produced by FastQC (version 0.11.5)