##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511855_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2282900 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.562953261202857 31.0 30.0 34.0 25.0 34.0 2 26.797936834727757 31.0 26.0 34.0 10.0 34.0 3 28.551983880152438 31.0 28.0 34.0 16.0 34.0 4 32.93413728152788 35.0 33.0 37.0 27.0 37.0 5 33.309462963774145 35.0 35.0 37.0 28.0 37.0 6 33.514874501730255 37.0 35.0 37.0 28.0 37.0 7 33.50380393359324 37.0 35.0 37.0 28.0 37.0 8 33.51876210083665 37.0 35.0 37.0 28.0 37.0 9 35.075035262166544 39.0 35.0 39.0 28.0 39.0 10 34.94695343641859 39.0 35.0 39.0 28.0 39.0 11 34.94787945157475 39.0 35.0 39.0 27.0 39.0 12 34.83954487712997 39.0 35.0 39.0 27.0 39.0 13 34.836430855490825 39.0 35.0 39.0 27.0 39.0 14 35.93927329274169 40.0 36.0 41.0 27.0 41.0 15 35.89640106881598 40.0 36.0 41.0 27.0 41.0 16 35.852058784878885 40.0 36.0 41.0 27.0 41.0 17 35.81036576284551 40.0 36.0 41.0 27.0 41.0 18 35.77467738402908 40.0 36.0 41.0 27.0 41.0 19 35.79937141355294 40.0 36.0 41.0 27.0 41.0 20 35.737381838889135 40.0 36.0 41.0 27.0 41.0 21 35.63062814840773 40.0 36.0 41.0 27.0 41.0 22 35.537156686670464 40.0 36.0 41.0 27.0 41.0 23 35.430597047614874 40.0 36.0 41.0 26.0 41.0 24 35.32640063077665 40.0 36.0 41.0 25.0 41.0 25 35.22930351745587 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8495022317742631 1101 2 3.307002362380981 1101 3 1.9881172564861131 1101 4 1.0527279171827146 1101 5 0.3616099528256811 1101 6 0.4593779151784503 1101 7 0.1827738694839951 1101 8 0.17755615620470167 1101 9 0.27856524814331607 1101 10 0.2493963476822998 1101 11 0.22824180549411466 1101 12 0.06078539737037403 1101 13 0.03849543563622149 1101 14 0.095609517746432 1101 15 0.28244685920356716 1101 16 0.1852609337515858 1101 17 0.16557568582599913 1101 18 0.35903449922261643 1101 19 0.3008854408794548 1101 20 0.29949575608462453 1101 21 0.3854524741507177 1101 22 0.25706402494184033 1101 23 0.0012358159164023164 1101 24 0.2327777728037006 1101 25 0.441434963297759 1102 1 1.24520126386291 1102 2 3.987644848498551 1102 3 2.344780440553297 1102 4 1.4937049875507498 1102 5 1.0660292034673944 1102 6 1.0001113394227445 1102 7 0.9622214045727517 1102 8 0.9102809939989385 1102 9 0.9678688114487741 1102 10 0.8953665080363962 1102 11 0.9036876452989517 1102 12 0.9759037845277803 1102 13 1.1268366400519056 1102 14 1.2469304179788665 1102 15 1.116957246638087 1102 16 1.1832233328839763 1102 17 1.287126218582216 1102 18 1.1530391643098028 1102 19 1.2216640285469822 1102 20 1.2014351955304363 1102 21 1.0364437924428245 1102 22 1.305432849037281 1102 23 1.3691659139810355 1102 24 1.1071910074288596 1102 25 1.2082048004883532 1103 1 1.4835835698157283 1103 2 4.076662023114469 1103 3 2.5901961559417046 1103 4 1.6452067602247524 1103 5 1.2640805213797748 1103 6 1.1135343262562145 1103 7 1.0754088250320635 1103 8 0.9134164466394736 1103 9 0.9552805790015597 1103 10 1.1427099662791633 1103 11 1.2022648145220316 1103 12 1.014685071767147 1103 13 1.246460899338878 1103 14 1.3646494045792394 1103 15 1.2441376554173544 1103 16 1.179315005242259 1103 17 1.2569011267631822 1103 18 1.3896836126721226 1103 19 1.476759694668928 1103 20 1.4451179918267627 1103 21 1.3747534036990388 1103 22 1.4592253441987921 1103 23 1.5066117324803585 1103 24 1.6167257663091377 1103 25 1.4425422293184198 1104 1 1.3898629717588733 1104 2 3.944674589300398 1104 3 2.1652474994385784 1104 4 1.5061151553478709 1104 5 1.2131520902796495 1104 6 1.0290113350248262 1104 7 0.9294630635430607 1104 8 0.9638476345145435 1104 9 0.9994070849207688 1104 10 1.1050079263980308 1104 11 1.0889024126561822 1104 12 1.076559121590364 1104 13 1.1728312953993694 1104 14 1.3384487258492044 1104 15 1.2708690054884286 1104 16 1.2603656509272128 1104 17 1.1330534391879254 1104 18 1.2114302735167257 1104 19 1.097546272166099 1104 20 1.028133583953604 1104 21 1.137996683080786 1104 22 1.141458370202379 1104 23 1.181701587952503 1104 24 1.1215502095046759 1104 25 1.104343712273213 1105 1 1.301539120717056 1105 2 3.893479890939304 1105 3 2.2811698326100647 1105 4 1.380693378095522 1105 5 0.9760147571433464 1105 6 0.7674735940972113 1105 7 0.7380577756262099 1105 8 0.7607526515087812 1105 9 0.8514659648492753 1105 10 0.8466218599129149 1105 11 0.8648430331041581 1105 12 0.7488555689856327 1105 13 0.9037316908760218 1105 14 0.9744048187932535 1105 15 1.0348237191900722 1105 16 1.0092157691479926 1105 17 0.9489722978648345 1105 18 1.0827831537407775 1105 19 0.9563087275694215 1105 20 1.011842162700603 1105 21 1.1552924091885828 1105 22 0.9390285989736569 1105 23 0.9931053629248865 1105 24 0.9870034859090708 1105 25 1.108213595530195 1106 1 1.051196250877343 1106 2 3.7928293959808315 1106 3 2.214997869019392 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0.16640007352966535 2314 5 -0.09163231750503797 2314 6 -0.23933772153084476 2314 7 -0.40625569218254753 2314 8 -0.49810458089445575 2314 9 -0.5589343811137724 2314 10 -0.6646350539675723 2314 11 -0.5545931090043368 2314 12 -0.6472811994074377 2314 13 -0.5945593431391956 2314 14 -1.3792892648620096 2314 15 -0.9007393181825449 2314 16 -0.7804304257965384 2314 17 -0.8678162584523506 2314 18 -0.8538354610081171 2314 19 -0.8725656684748699 2314 20 -0.7394208983459976 2314 21 -0.8471829348913076 2314 22 -0.6846780749386809 2314 23 -0.6862139964785356 2314 24 -0.7354718043662984 2314 25 -0.6904909262014485 2315 1 -4.689893846028166 2315 2 -13.749514348390361 2315 3 -7.373900866525837 2315 4 -3.430730423946901 2315 5 -2.2700509668681725 2315 6 -1.716636447798571 2315 7 -1.522876696748753 2315 8 -1.4697844655375718 2315 9 -1.455214121560779 2315 10 -1.6322341887909317 2315 11 -1.6515313450052957 2315 12 -1.6023544990950143 2315 13 -1.5645329071862975 2315 14 -1.6318775849773672 2315 15 -1.7208642881416907 2315 16 -1.7298896903963907 2315 17 -1.5914836460995545 2315 18 -1.5908025362304201 2315 19 -1.836761775179994 2315 20 -1.6474285888050275 2315 21 -1.7890238385202437 2315 22 -1.6453894423984252 2315 23 -1.8056420185885997 2315 24 -1.8777068440202243 2315 25 -1.8985466820293695 2316 1 -1.368110904566933 2316 2 -1.2234370524610512 2316 3 -0.8491215588434287 2316 4 -0.8977631647230453 2316 5 -1.1470576645414496 2316 6 -1.2806029655599644 2316 7 -1.235106170187855 2316 8 -1.4991092444775802 2316 9 -1.4232391964729985 2316 10 -1.609413812667249 2316 11 -1.7620052069216996 2316 12 -1.4090297446661566 2316 13 -1.4073835545871987 2316 14 -1.8211060350757151 2316 15 -2.3478673704195074 2316 16 -2.019521471560793 2316 17 -2.030018369215412 2316 18 -1.8524737068219324 2316 19 -1.9179052205905833 2316 20 -2.0550594017787773 2316 21 -2.010040928923516 2316 22 -1.967958579176802 2316 23 -1.9254363699927879 2316 24 -1.7210956338431984 2316 25 -1.8265983515454636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83008.0 3 2.0 4 744.0 5 3791.0 6 4548.0 7 4031.0 8 3602.0 9 3598.0 10 3419.0 11 3384.0 12 3259.0 13 3276.0 14 3357.0 15 3395.0 16 3561.0 17 3863.0 18 4332.0 19 5497.0 20 7095.0 21 9084.0 22 11280.0 23 13401.0 24 15907.0 25 17900.0 26 20029.0 27 22807.0 28 26091.0 29 31299.0 30 38912.0 31 49806.0 32 63987.0 33 83519.0 34 113682.0 35 162147.0 36 257136.0 37 342889.0 38 855262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13500372333436 19.687546541679442 13.059047702483683 25.11840203250252 2 15.33496946394316 21.654964346012882 41.34077720221666 21.669288987827297 3 18.076394060186605 25.852599763458763 30.352052214288843 25.718953962065793 4 11.656620964562618 17.710061763546367 40.07783958999518 30.55547768189583 5 12.230292405806853 38.715154350474336 36.74751597646666 12.307037267252152 6 29.841288475454732 38.597493346135856 19.200581369881256 12.36063680852816 7 26.733015024749225 32.10933461824872 23.624206053703624 17.533444303298438 8 23.598186517149237 38.60528275439135 21.55898199658329 16.23754873187612 9 26.17013447807613 15.295369924219196 21.350300056945112 37.18419554075956 10 14.386044066757195 29.483464015068556 35.96123351876999 20.169258399404267 11 32.91042095580183 22.412282622979543 26.60668448026633 18.070611940952297 12 23.100661439397257 24.93696613955933 33.29011345218801 18.672258968855402 13 28.693353798549037 22.919289429488305 26.32307707133652 22.064279700626134 14 19.74541153795611 23.60804240220772 28.739541810854615 27.907004248981558 15 21.6554382583556 33.295895571422314 23.81256296815454 21.236103202067547 16 20.540890972009286 28.78330194051426 30.011564238468612 20.66424284900784 17 20.537398007524676 29.788220101809593 29.064969869319096 20.609412021346635 18 20.175434754040914 27.898681501598844 32.158219808138774 19.76766393622147 19 22.882291409317652 27.254946795555814 28.5119050747911 21.350856720335436 20 22.88014129471091 28.11027084990591 29.442180255886402 19.56740759949678 21 21.80796588178297 27.42914013227117 28.083268284093027 22.679625701852828 22 22.269211073540475 29.555797654733574 28.340760579828995 19.834230691896966 23 20.86301060234124 29.10010635632347 29.190780816613316 20.846102224721978 24 22.361745429186445 27.439774948376023 30.065041391170876 20.133438231266652 25 21.66156638845109 28.402300250297653 29.369454925834976 20.566678435416286 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 206.0 1 206.0 2 389.5 3 573.0 4 573.0 5 573.0 6 1507.0 7 2441.0 8 2441.0 9 2441.0 10 3741.0 11 5041.0 12 5041.0 13 5041.0 14 8521.5 15 12002.0 16 12002.0 17 12002.0 18 22001.5 19 32001.0 20 32001.0 21 32001.0 22 51262.0 23 70523.0 24 70523.0 25 70523.0 26 102886.5 27 135250.0 28 135250.0 29 135250.0 30 174388.0 31 213526.0 32 213526.0 33 213526.0 34 237261.5 35 260997.0 36 260997.0 37 260997.0 38 272475.5 39 283954.0 40 283954.0 41 283954.0 42 290690.0 43 297426.0 44 297426.0 45 297426.0 46 295731.0 47 294036.0 48 294036.0 49 294036.0 50 273953.5 51 253871.0 52 253871.0 53 253871.0 54 215699.0 55 177527.0 56 177527.0 57 177527.0 58 147502.0 59 117477.0 60 117477.0 61 117477.0 62 92984.5 63 68492.0 64 68492.0 65 68492.0 66 51402.5 67 34313.0 68 34313.0 69 34313.0 70 24660.0 71 15007.0 72 15007.0 73 15007.0 74 10310.5 75 5614.0 76 5614.0 77 5614.0 78 3713.0 79 1812.0 80 1812.0 81 1812.0 82 1202.5 83 593.0 84 593.0 85 593.0 86 375.5 87 158.0 88 158.0 89 158.0 90 97.5 91 37.0 92 37.0 93 37.0 94 27.5 95 18.0 96 18.0 97 18.0 98 11.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.509571159490123 3 0.0 4 0.0 5 4.818432695256034E-4 6 7.008629374917868E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 8.760786718647335E-5 14 0.0 15 0.0 16 0.0 17 4.818432695256034E-4 18 0.0 19 3.504314687458934E-4 20 5.256472031188401E-4 21 6.132550703053135E-4 22 0.0038985500897980637 23 3.504314687458934E-4 24 0.0020149809452888866 25 7.884708046782601E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2282900.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.8929940644819 #Duplication Level Percentage of deduplicated Percentage of total 1 87.36056711963693 65.4269443476074 2 9.234591247837818 13.832123750244685 3 1.8360416214834776 4.125199627797114 4 0.6199281341946685 1.8571309629858659 5 0.2593791395838957 0.971284018065356 6 0.1522956818795099 0.6843527759429014 7 0.10365170721029644 0.5433950685011903 8 0.07272478421641901 0.4357261466128797 9 0.05190434047802935 0.349854431700771 >10 0.2677274966653012 3.634292467911275 >50 0.019483908099869756 1.0246093996434005 >100 0.017676331369922596 2.67926220191337 >500 0.0020734861328750047 1.088757462152465 >1k 0.001658788906298098 2.106614136800818 >5k 2.962123046960889E-4 1.2404532021204147 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6146 0.2692189758640326 No Hit CTGTAGGACGTGGAATATGGCAAGA 5586 0.24468877305182007 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5579 0.2443821455166674 No Hit CTTTAGGACGTGAAATATGGCGAGG 5428 0.23776775154408866 No Hit GTATCAACGCAGAGTACTTTTTTTT 5219 0.2286127294231022 No Hit GGTATCAACGCAGAGTACTTTTTTT 3429 0.15020368829120856 No Hit TATCAACGCAGAGTACTTTTTTTTT 3292 0.14420254938893512 No Hit GTCCTACAGTGTGCATTTCTCATTT 2955 0.12944062376801438 No Hit CTGAAGGACCTGGAATATGGCGAGA 2811 0.12313285733058829 No Hit CTGTAGGACCTGGAATATGGCGAGA 2296 0.1005738315300714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.3803933593236675E-5 0.0 0.0 0.0 0.0 7 4.3803933593236675E-5 0.0 0.0 0.0 0.0 8 4.3803933593236675E-5 0.0 0.0 0.0 0.0 9 4.3803933593236675E-5 0.0 0.0 0.0 0.0 10 4.3803933593236675E-5 0.0 0.0 4.3803933593236675E-5 0.0 11 4.3803933593236675E-5 0.0 0.0 4.3803933593236675E-5 0.0 12 4.3803933593236675E-5 0.0 0.0 4.3803933593236675E-5 0.0 13 4.3803933593236675E-5 0.0 0.0 8.760786718647335E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1075 0.0 14.133407 1 CGCTTAC 45 0.008223939 13.327554 1 GCGCCAC 60 4.2993322E-4 12.593135 13 GGCGAAA 85 4.208814E-6 12.223284 19 TCCAACG 210 0.0 12.143911 18 TAGGACC 1185 0.0 11.875804 4 GGCGAGG 1680 0.0 11.806581 19 GCCCGGA 70 9.7521645E-4 11.423618 1 GCCGGTT 110 5.3599433E-7 11.162096 11 GGACGTG 3440 0.0 11.00984 6 AGGACGT 3480 0.0 10.991852 5 AATGTCC 880 0.0 10.94744 8 TGGCGAA 105 3.7164318E-6 10.794587 18 GTATCAA 2325 0.0 10.79102 1 CCGGTTT 125 2.42846E-7 10.578234 12 TGTCGAG 180 4.0017767E-11 10.494739 18 GACGTGA 2035 0.0 10.489122 7 TTAGGAC 2300 0.0 10.471465 3 GTCGAGG 145 1.5801561E-8 10.422361 19 TGTAGGA 3460 0.0 10.371224 2 >>END_MODULE