FastQCFastQC Report
Thu 26 May 2016
SRR1511855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282900
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68780.3012834552542818No Hit
GTATCAACGCAGAGTACTTTTTTTT67020.29357396294187216No Hit
CTGTAGGACGTGGAATATGGCAAGA63390.2776731350475273No Hit
GTCCTAAAGTGTGTATTTCTCATTT62910.2755705462350519No Hit
CTTTAGGACGTGAAATATGGCGAGG59810.2619913268211485No Hit
GGTATCAACGCAGAGTACTTTTTTT44370.19435805335319112No Hit
TATCAACGCAGAGTACTTTTTTTTT43360.18993385606027421No Hit
GTCCTACAGTGTGCATTTCTCATTT33710.1476630601428008No Hit
CTGAAGGACCTGGAATATGGCGAGA31830.13942792062727233No Hit
CTGTAGGACCTGGAATATGGCGAGA27550.12067983704936702No Hit
TTTCTAAATTTTCCACCTTTTTCAG25260.11064873625651585No Hit
ATTTAGAAATGTCCACTGTAGGACG23470.10280783214332648No Hit
GTCCTTCAGTGTGCATTTCTCATTT23340.10223838100661439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT456.778489E-414.7736228
GGTATCA12050.013.69636251
TAGGACC12100.012.7964444
GCGTGCG658.044912E-411.6890199
GGCGAGG15950.011.61166319
GGCCGTG855.340443E-511.17332657
AGGCCGT855.340443E-511.17332656
GTACCGT600.00588940811.0802156
ATAGGAC3150.010.55466653
TCCAACG1451.4495527E-810.48026918
TAGAAAT10300.010.42145254
GTATCAA24600.010.3931191
CCACCTT8900.010.35102113
GGACCTG23650.010.2804066
AGGACCT24550.010.2537195
CTGTAGG38350.010.1991671
TGGCGAG36150.010.19398318
TGTAGGA38450.010.1785972
GCGCCAC856.612545E-410.05599413
ACCTTTT9300.010.00793715
Produced by FastQC (version 0.11.5)