FastQCFastQC Report
Thu 26 May 2016
SRR1511854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028611
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54860.27043134440264793No Hit
GGTATCAACGCAGAGTACTTTTTTT35220.17361633156874334No Hit
TATCAACGCAGAGTACTTTTTTTTT33980.16750377475030945No Hit
GTCCTACAGTGGACATTTCTAAATT32650.16094756461440857No Hit
CTGTAGGACGTGGAATATGGCAAGA31890.15720115882246521No Hit
GTCCTAAAGTGTGTATTTCTCATTT29930.14753937546429552No Hit
CTTTAGGACGTGAAATATGGCGAGG28410.14004656388040881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4950.013.7381794
GGTATCA12000.013.3250751
CGGTAGG955.634265E-712.62375451
GTACTAG1551.2914825E-1011.605711
GGACCGT700.001565756310.7942846
GGCGAGG8350.010.74633719
CGTTTAT750.001737770510.660061
GTATAGA2600.010.3781831
GTATCAA24350.010.3016221
GGACGTG19950.010.1314776
GACGTGG9450.010.0946557
TATACTG3650.010.0919165
AACAGCG750.002778801810.0746667
TTATACT2350.010.0478724
GTCCTAA10100.09.9938071
TAGGACT2650.09.9796214
GTAGGAC17700.09.9786153
ATAGGAC1804.3837645E-109.9697223
AGGACGT20500.09.9518045
CTAAGAC1901.10958354E-109.9421043
Produced by FastQC (version 0.11.5)