FastQCFastQC Report
Thu 26 May 2016
SRR1511854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028611
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71680.3533452199559206No Hit
GGTATCAACGCAGAGTACTTTTTTT47370.2335095294267851No Hit
TATCAACGCAGAGTACTTTTTTTTT46800.2306997250828276No Hit
GTCCTACAGTGGACATTTCTAAATT36130.178102159556465No Hit
CTGTAGGACGTGGAATATGGCAAGA34920.17213748717718674No Hit
GTCCTAAAGTGTGTATTTCTCATTT33000.1626728830712246No Hit
CTTTAGGACGTGAAATATGGCGAGG30740.1515322553214983No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22400.11042038123622519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.013.0504041
TAGGACC4900.011.8259794
TTATACA3750.010.8928244
GACAGCG700.001495395310.853967
GTATTAG1553.417881E-910.4632371
CTAGGAC2200.010.36315253
ACTGTTC4150.010.2981858
AGGACGT20700.010.1879295
GTAGGAC19650.010.1521983
GGACGTG20250.010.13036256
TGTAGGA20800.010.093282
AGGACCT11400.010.0828625
GACGTGG11800.010.0606087
TAGAAAT6250.010.031534
CTTAGAC2201.8189894E-129.9313543
TAGGACT2500.09.8795394
GGCGAGG8850.09.87329819
AGTCGTC1554.0825398E-89.8035768
GTATCAA23200.09.7867351
TAGGACG21950.09.7375864
Produced by FastQC (version 0.11.5)