FastQCFastQC Report
Thu 26 May 2016
SRR1511853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911021
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57400.30036299967399627No Hit
GGTATCAACGCAGAGTACTTTTTTT38900.2035561095351647No Hit
TATCAACGCAGAGTACTTTTTTTTT36390.19042176930551782No Hit
GTCCTACAGTGGACATTTCTAAATT35570.18613086931017503No Hit
CTGTAGGACGTGGAATATGGCAAGA33660.17613621200394972No Hit
GTCCTAAAGTGTGTATTTCTCATTT31610.16540896201559271No Hit
CTTTAGGACGTGAAATATGGCGAGG30470.15944356446109173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.014.2353871
CCAACGA802.1330216E-612.98782819
TACGGTG550.00320411712.0214775
TATGTCG1052.930974E-711.69466816
GCGCCAC1157.667222E-811.49850213
GTCGAGG1105.3520125E-711.16309319
GTCCTAG3050.011.1378371
TTACACG600.006133806411.0196874
CCGTCCA600.00613500111.0193989
GCGACTA700.001564725110.79507716
GTCTAAG2051.8189894E-1210.7223661
GGCGAGG9500.010.63883419
TGCGCCA1606.020855E-1010.62584912
GTATCAA23750.010.6012911
GGCGTGC901.0091456E-410.4946668
TAGGACC6500.010.4626474
GTGCGCC1451.5796104E-810.42228811
CCAAGAC2500.010.2010823
ATGTCGA1304.1562816E-710.17228517
GTATAAG2102.3646862E-119.9912961
Produced by FastQC (version 0.11.5)