##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511853_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1911021 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.029907572967538 31.0 30.0 34.0 25.0 34.0 2 27.23961013510579 31.0 27.0 34.0 10.0 34.0 3 29.008693782014955 31.0 28.0 34.0 16.0 34.0 4 33.459971397488566 35.0 33.0 37.0 28.0 37.0 5 33.83236866575511 35.0 35.0 37.0 30.0 37.0 6 34.049746182799666 37.0 35.0 37.0 30.0 37.0 7 34.053453101771254 37.0 35.0 37.0 30.0 37.0 8 34.07591282356395 37.0 35.0 37.0 30.0 37.0 9 35.67754985424022 39.0 37.0 39.0 30.0 39.0 10 35.56070393784265 39.0 35.0 39.0 30.0 39.0 11 35.56378762975394 39.0 35.0 39.0 30.0 39.0 12 35.46117441932873 39.0 35.0 39.0 30.0 39.0 13 35.45546490593248 39.0 35.0 39.0 30.0 39.0 14 36.60161452961532 40.0 37.0 41.0 30.0 41.0 15 36.55637588493271 40.0 37.0 41.0 30.0 41.0 16 36.51089234498208 40.0 37.0 41.0 30.0 41.0 17 36.471745208451395 40.0 37.0 41.0 30.0 41.0 18 36.44632842862533 40.0 37.0 41.0 30.0 41.0 19 36.482022437220735 40.0 37.0 41.0 30.0 41.0 20 36.42407697246655 40.0 37.0 41.0 30.0 41.0 21 36.32599275465837 40.0 37.0 41.0 29.0 41.0 22 36.24378015730858 40.0 37.0 41.0 29.0 41.0 23 36.13900527519059 40.0 36.0 41.0 29.0 41.0 24 36.03585674882694 40.0 36.0 41.0 29.0 41.0 25 35.9378159632992 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5094951063050459 1101 2 3.2120327041337013 1101 3 1.7134136654626708 1101 4 0.7972053356558746 1101 5 0.05422824345136945 1101 6 0.11443082032269558 1101 7 -0.05763409904945149 1101 8 -0.016238285927251184 1101 9 0.03717093347162148 1101 10 -0.12153434754760184 1101 11 -0.0885764194224663 1101 12 -0.1170359926109441 1101 13 -0.2402060937306132 1101 14 -0.2019915292062251 1101 15 0.08764593217340888 1101 16 0.04790557696392028 1101 17 0.08053076647001944 1101 18 0.14440383138915536 1101 19 0.31533256118859043 1101 20 0.19004685091076112 1101 21 0.150079530590304 1101 22 0.23866342461425916 1101 23 0.04650721590542162 1101 24 0.2718061111217054 1101 25 0.20971528769860726 1102 1 1.2133266454506781 1102 2 3.8697315417985827 1102 3 2.1766561388919 1102 4 1.2517349599647858 1102 5 0.9371551637297344 1102 6 0.6616487410550462 1102 7 0.5785469242283412 1102 8 0.5285790925064262 1102 9 0.6452917317452247 1102 10 0.6261661692530822 1102 11 0.5810753775637991 1102 12 0.6610631012776196 1102 13 0.7646260859742284 1102 14 1.0137649836640392 1102 15 0.7316318274326505 1102 16 0.6910270282347781 1102 17 0.8482440944549907 1102 18 0.8054174075130831 1102 19 0.8616860379325573 1102 20 0.9730030005901753 1102 21 0.9403990017247139 1102 22 0.8753046554829922 1102 23 0.9812696803792633 1102 24 0.6875912314908703 1102 25 0.7516346021136826 1103 1 0.90165883961361 1103 2 3.6400890887786375 1103 3 2.1288894689523055 1103 4 1.1749647257087759 1103 5 0.8340807382481614 1103 6 0.7286713627230483 1103 7 0.7185835365611908 1103 8 0.5542868477941951 1103 9 0.5597421002994167 1103 10 0.7724890320693234 1103 11 0.7781496320903685 1103 12 0.7579434267403542 1103 13 0.8472078369410951 1103 14 1.1190074871443088 1103 15 0.7984529891195393 1103 16 0.8192134085867764 1103 17 0.9417939750248152 1103 18 1.041542804594208 1103 19 1.0690655742377757 1103 20 1.1378754341600157 1103 21 1.1921569307161093 1103 22 0.8777358389459877 1103 23 1.1859279181382618 1103 24 1.1108971008081596 1103 25 1.1517897326543434 1104 1 1.2104701297884688 1104 2 3.6614254166843097 1104 3 2.113263535637291 1104 4 1.421089103996536 1104 5 1.0901445481246768 1104 6 0.7826931291225137 1104 7 0.6667997351268227 1104 8 0.6407232668909657 1104 9 0.87535887468308 1104 10 0.952939890420474 1104 11 0.9143031198512475 1104 12 0.9046097748354924 1104 13 0.9405731867127187 1104 14 1.1113737169697728 1104 15 1.0319420242858541 1104 16 0.9941131581099114 1104 17 0.9478627625814084 1104 18 0.933606789685058 1104 19 1.0157128080413997 1104 20 1.1121229084851407 1104 21 1.011988804593642 1104 22 0.8717108549741184 1104 23 1.013019240648127 1104 24 1.0927783453078277 1104 25 1.1440683553103526 1105 1 1.2685029306554725 1105 2 3.897709886931043 1105 3 2.238328274190586 1105 4 1.4622838519757053 1105 5 1.1146427048588805 1105 6 0.9485305835187816 1105 7 0.7945522448945894 1105 8 0.8181102850648188 1105 9 0.8449058003921905 1105 10 0.7553494439340014 1105 11 0.8414933122361461 1105 12 0.715274100473728 1105 13 0.8785603434072229 1105 14 1.0045407667004511 1105 15 1.134664058039597 1105 16 0.9695944144422342 1105 17 0.9847048446262221 1105 18 1.0490624299901583 1105 19 0.956802709709379 1105 20 1.0641130032291457 1105 21 1.2605960250194101 1105 22 1.1077665161602468 1105 23 0.9312764684998953 1105 24 0.990705682255637 1105 25 1.2212346521474657 1106 1 0.9406846661495756 1106 2 3.604490581971696 1106 3 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-1.0933606229792474 2315 8 -1.117059424860912 2315 9 -1.1265582592956846 2315 10 -1.204168591706349 2315 11 -1.1901808041484472 2315 12 -1.1807436098575863 2315 13 -1.1200775834024697 2315 14 -1.2792554630944792 2315 15 -1.3087380830853803 2315 16 -1.3251659975595587 2315 17 -1.1747439044126367 2315 18 -1.1791220075631799 2315 19 -1.314892023663944 2315 20 -1.2225949537455634 2315 21 -1.3761039048490318 2315 22 -1.3933361547537402 2315 23 -1.3010127777125788 2315 24 -1.5144747586437362 2315 25 -1.5312279795604482 2316 1 -1.5001250359476437 2316 2 -1.2792804490100522 2316 3 -0.868489160560582 2316 4 -1.138097451028699 2316 5 -1.3171895218191807 2316 6 -1.320103957274064 2316 7 -1.2876514235032843 2316 8 -1.499594007881079 2316 9 -1.3945393379945017 2316 10 -1.6120162782407377 2316 11 -1.7089898907090912 2316 12 -1.5553326972384554 2316 13 -1.4957214263646534 2316 14 -1.8372842283689081 2316 15 -2.477642825137046 2316 16 -2.0260929170941537 2316 17 -1.8812737235152568 2316 18 -1.9310572202629501 2316 19 -1.8815511131819491 2316 20 -2.1086829996634933 2316 21 -2.033809339519408 2316 22 -2.1258144449862897 2316 23 -2.0079648700051322 2316 24 -1.896244551128099 2316 25 -2.117388712522839 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50996.0 3 1.0 4 483.0 5 2382.0 6 2949.0 7 2672.0 8 2477.0 9 2395.0 10 2349.0 11 2270.0 12 2225.0 13 2320.0 14 2230.0 15 2293.0 16 2452.0 17 2651.0 18 3068.0 19 3724.0 20 4749.0 21 6015.0 22 7606.0 23 9374.0 24 10835.0 25 12601.0 26 14462.0 27 16507.0 28 19661.0 29 23916.0 30 30389.0 31 39052.0 32 51693.0 33 69970.0 34 97651.0 35 141793.0 36 224189.0 37 304922.0 38 735699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18205870055849 19.607424512865112 13.198232777138502 25.012284009437884 2 15.716748816188945 21.628027332128536 40.60797104056369 22.047252811118828 3 18.001686009729877 25.737917061089334 30.42337054380878 25.83702638537201 4 11.817504883515147 17.67651951496085 39.64577050696983 30.860205094554168 5 12.51207476674638 38.349563843203335 36.4443409505968 12.694020439453482 6 29.98024083574567 38.28437498037689 19.12525928670254 12.610124897174902 7 26.68986892347075 32.1898608126232 23.499951073274445 17.620319190631605 8 23.97749684592686 37.5587709397228 21.585058458279633 16.878673756070707 9 26.08333451071443 15.37251552965666 21.377839385333807 37.1663105742951 10 14.66865094627427 29.358861048622696 35.40400654937858 20.568481455724452 11 32.821408032669446 22.8321928435114 25.942833699891317 18.403565423927837 12 22.740409446050045 25.25142319210516 32.77326622784365 19.234901134001145 13 28.334711306004124 22.78317338384737 26.439807014055322 22.442308296093184 14 20.31589396453519 23.09351911883752 28.781734999249093 27.808851917378195 15 22.204779539314327 31.90603347634589 24.099578183599238 21.789608800740545 16 21.04131770399174 28.775455633402245 28.962319095394555 21.22090756721145 17 20.805028115963566 29.35535794086281 28.70177821826528 21.137835724908346 18 20.51317070822351 27.874942242916223 31.153032855211954 20.458854193648317 19 22.593519576225642 27.40111678755343 28.8116222932606 21.19374134296032 20 22.760824608480515 27.86800319829871 29.238897005571907 20.132275187648872 21 22.195348749589876 27.30248302863455 28.27798659663749 22.22418162513808 22 22.285937062552822 28.77194267757576 28.47737637660948 20.464743883261946 23 21.33035900237465 28.445362628714122 28.94043907488051 21.28383929403072 24 22.130657705112252 27.37493576587486 29.749112757970003 20.745293771042892 25 21.881953151327785 27.949758346921307 29.1804726730033 20.98781582874761 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 341.0 1 341.0 2 489.5 3 638.0 4 638.0 5 638.0 6 1695.0 7 2752.0 8 2752.0 9 2752.0 10 3937.0 11 5122.0 12 5122.0 13 5122.0 14 8237.5 15 11353.0 16 11353.0 17 11353.0 18 19890.0 19 28427.0 20 28427.0 21 28427.0 22 44037.5 23 59648.0 24 59648.0 25 59648.0 26 84134.5 27 108621.0 28 108621.0 29 108621.0 30 137600.0 31 166579.0 32 166579.0 33 166579.0 34 186799.0 35 207019.0 36 207019.0 37 207019.0 38 218273.5 39 229528.0 40 229528.0 41 229528.0 42 236047.5 43 242567.0 44 242567.0 45 242567.0 46 243196.5 47 243826.0 48 243826.0 49 243826.0 50 229973.5 51 216121.0 52 216121.0 53 216121.0 54 187453.5 55 158786.0 56 158786.0 57 158786.0 58 133399.0 59 108012.0 60 108012.0 61 108012.0 62 86560.0 63 65108.0 64 65108.0 65 65108.0 66 49316.0 67 33524.0 68 33524.0 69 33524.0 70 24096.5 71 14669.0 72 14669.0 73 14669.0 74 10110.5 75 5552.0 76 5552.0 77 5552.0 78 3762.0 79 1972.0 80 1972.0 81 1972.0 82 1286.0 83 600.0 84 600.0 85 600.0 86 394.0 87 188.0 88 188.0 89 188.0 90 118.0 91 48.0 92 48.0 93 48.0 94 31.0 95 14.0 96 14.0 97 14.0 98 10.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.460745852609678 3 0.0 4 0.0 5 5.7560853596062E-4 6 4.709524385132346E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 5.232804872369273E-5 14 0.0 15 0.0 16 0.0 17 3.662963410658491E-4 18 0.0 19 2.093121948947709E-4 20 2.616402436184636E-4 21 4.186243897895418E-4 22 0.003976931703000648 23 1.5698414617107818E-4 24 0.0022501060951187874 25 8.895768283027763E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1911021.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.71929007487337 #Duplication Level Percentage of deduplicated Percentage of total 1 86.88658331249046 66.65876988765608 2 9.696022058610765 14.877438577738603 3 1.949559064735002 4.4870636221651035 4 0.5946899996491676 1.8249677835084335 5 0.2622756564058104 1.0060801081687594 6 0.1504538981170954 0.692562975152453 7 0.09223762783350448 0.495348372790379 8 0.0639888496882416 0.39273432966317423 9 0.04195778168330189 0.2897074101473501 >10 0.22756879573622296 3.1236175179631713 >50 0.01789443130516004 0.9668469290242093 >100 0.013539517764214026 2.0199122812200514 >500 0.001992365392638257 1.0118859232233497 >1k 0.0011679383336155207 1.8505210375272616 >5k 6.870225491856004E-5 0.30254324405156746 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5740 0.30036299967399627 No Hit GGTATCAACGCAGAGTACTTTTTTT 3890 0.2035561095351647 No Hit TATCAACGCAGAGTACTTTTTTTTT 3639 0.19042176930551782 No Hit GTCCTACAGTGGACATTTCTAAATT 3557 0.18613086931017503 No Hit CTGTAGGACGTGGAATATGGCAAGA 3366 0.17613621200394972 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3161 0.16540896201559271 No Hit CTTTAGGACGTGAAATATGGCGAGG 3047 0.15944356446109173 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 5.232804872369273E-5 0.0 0.0 0.0 0.0 10 5.232804872369273E-5 0.0 0.0 0.0 0.0 11 5.232804872369273E-5 0.0 0.0 1.5698414617107818E-4 0.0 12 5.232804872369273E-5 0.0 0.0 1.5698414617107818E-4 0.0 13 5.232804872369273E-5 0.0 0.0 1.5698414617107818E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1130 0.0 14.235387 1 CCAACGA 80 2.1330216E-6 12.987828 19 TACGGTG 55 0.003204117 12.021477 5 TATGTCG 105 2.930974E-7 11.694668 16 GCGCCAC 115 7.667222E-8 11.498502 13 GTCGAGG 110 5.3520125E-7 11.163093 19 GTCCTAG 305 0.0 11.137837 1 TTACACG 60 0.0061338064 11.019687 4 CCGTCCA 60 0.006135001 11.019398 9 GCGACTA 70 0.0015647251 10.795077 16 GTCTAAG 205 1.8189894E-12 10.722366 1 GGCGAGG 950 0.0 10.638834 19 TGCGCCA 160 6.020855E-10 10.625849 12 GTATCAA 2375 0.0 10.601291 1 GGCGTGC 90 1.0091456E-4 10.494666 8 TAGGACC 650 0.0 10.462647 4 GTGCGCC 145 1.5796104E-8 10.422288 11 CCAAGAC 250 0.0 10.201082 3 ATGTCGA 130 4.1562816E-7 10.172285 17 GTATAAG 210 2.3646862E-11 9.991296 1 >>END_MODULE