FastQCFastQC Report
Thu 26 May 2016
SRR1511853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911021
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71270.37294200325375804No Hit
GGTATCAACGCAGAGTACTTTTTTT51560.26980341921935974No Hit
TATCAACGCAGAGTACTTTTTTTTT48730.25499458143055465No Hit
GTCCTACAGTGGACATTTCTAAATT36140.18911356808742552No Hit
CTGTAGGACGTGGAATATGGCAAGA35820.18743907052826736No Hit
GTCCTAAAGTGTGTATTTCTCATTT32560.17038012664434352No Hit
CTTTAGGACGTGAAATATGGCGAGG32510.17011848640072505No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23120.12098244864917759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG755.8631485E-815.19675419
GGTATCA14850.013.8058211
TAGGACC5700.012.8339534
TATGTCG1155.349648E-912.38768716
TGTCGAG1251.8380888E-811.39756618
GGCGAGG9000.010.86990219
CAAGTCG1803.6379788E-1110.5524746
AAGTCGT2100.010.4017247
TGGTATC3600.010.2921632
GACGTGG10700.010.2072997
TGTCCAC7000.010.03992510
GTATAAG2105.456968E-129.9897271
GGACGTG22300.09.9231116
AGGACCT12750.09.9102825
GTGGTAT4050.09.8888221
AATGTCC6350.09.8711338
AGGACGT23200.09.8690115
GTCCACT7500.09.75048611
GTAGGAC20750.09.66070653
GTATCAA28700.09.5688731
Produced by FastQC (version 0.11.5)