##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511853_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1911021 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.85198488138016 31.0 31.0 34.0 27.0 34.0 2 31.051297186163836 31.0 31.0 34.0 27.0 34.0 3 31.155406978782548 31.0 31.0 34.0 27.0 34.0 4 34.75529206638755 37.0 35.0 37.0 32.0 37.0 5 34.39603803411894 37.0 35.0 37.0 30.0 37.0 6 34.33022557051963 37.0 35.0 37.0 30.0 37.0 7 34.16913471908472 37.0 35.0 37.0 30.0 37.0 8 34.07714828879431 37.0 35.0 37.0 30.0 37.0 9 35.56988698711317 39.0 35.0 39.0 30.0 39.0 10 35.374148688057325 39.0 35.0 39.0 30.0 39.0 11 35.36171502040009 39.0 35.0 39.0 30.0 39.0 12 35.1968555028961 39.0 35.0 39.0 29.0 39.0 13 35.17948939336616 39.0 35.0 39.0 29.0 39.0 14 36.18822189813717 39.0 36.0 41.0 29.0 41.0 15 36.13125967741851 39.0 36.0 41.0 28.0 41.0 16 36.02369728014501 39.0 36.0 41.0 27.0 41.0 17 35.96618142867085 39.0 36.0 41.0 27.0 41.0 18 35.89418221987095 39.0 36.0 41.0 27.0 41.0 19 35.922264590499005 39.0 36.0 41.0 27.0 41.0 20 35.79105305488532 39.0 36.0 41.0 27.0 41.0 21 35.676057981571105 39.0 36.0 41.0 27.0 41.0 22 35.76057196650377 39.0 36.0 41.0 27.0 41.0 23 35.65383164287572 39.0 36.0 41.0 27.0 41.0 24 35.5389433187809 39.0 36.0 41.0 27.0 41.0 25 35.09491784757991 39.0 35.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3171813976213578 1101 2 0.2592133900850264 1101 3 0.3975162994774699 1101 4 0.354961028732113 1101 5 0.528325113895022 1101 6 0.5880668683057095 1101 7 0.5185021338979681 1101 8 0.42230648084890277 1101 9 0.46270955571824857 1101 10 0.5028230481636271 1101 11 0.250813919260537 1101 12 0.5741808350016413 1101 13 0.4300821947807236 1101 14 0.36979831050008727 1101 15 0.23827020373172303 1101 16 0.3001768580775277 1101 17 0.33370354090717314 1101 18 0.25698011898679596 1101 19 0.32031123201490885 1101 20 0.4425571108044295 1101 21 0.4587772167810371 1101 22 0.15920660595602953 1101 23 0.15974810753380808 1101 24 -0.07677926242971012 1101 25 -0.024120696418613363 1102 1 0.3595764671811139 1102 2 0.40738157730245206 1102 3 0.5161379155523953 1102 4 0.3925612327160124 1102 5 0.45291947821774414 1102 6 0.510814183243177 1102 7 0.648262017477883 1102 8 0.5132472322310804 1102 9 0.6678191037819516 1102 10 0.8138425323579881 1102 11 0.6902251023049928 1102 12 0.8015917941613253 1102 13 0.8804699507712002 1102 14 0.9706117585121774 1102 15 1.0881368057205663 1102 16 1.128463455464292 1102 17 1.1756968616780838 1102 18 1.059457777152062 1102 19 1.063689528873681 1102 20 1.0801560709840388 1102 21 1.0042600305151268 1102 22 0.691822264208362 1102 23 0.9821079774318804 1102 24 0.937760809835325 1102 25 1.0063711659917516 1103 1 0.556896350359743 1103 2 0.48378098537870784 1103 3 0.5577371773318482 1103 4 0.486567416965471 1103 5 0.7284407876949075 1103 6 0.6015677738542564 1103 7 0.6050126268625533 1103 8 0.4673432727162776 1103 9 0.7246305006972023 1103 10 0.715354657929204 1103 11 0.8690186112262737 1103 12 0.8590507864102008 1103 13 0.5449412120600243 1103 14 0.9335434397465647 1103 15 1.0073405494444003 1103 16 0.8000480970959032 1103 17 0.8292517882802386 1103 18 0.967267910334904 1103 19 1.0150506071230936 1103 20 0.8633606637631885 1103 21 0.819738575821539 1103 22 1.0088306661064053 1103 23 0.9590878381067327 1103 24 1.1574770690221712 1103 25 1.0146173615280532 1104 1 0.5560601001058814 1104 2 0.5390332342945712 1104 3 0.5593857849751736 1104 4 0.5348748066302136 1104 5 0.4883971433959431 1104 6 0.5084652455909904 1104 7 0.40565454848599103 1104 8 0.48854818986777104 1104 9 0.7338292534896809 1104 10 0.6048621065296729 1104 11 0.6998292562937891 1104 12 0.6760394094246038 1104 13 0.8251328328324803 1104 14 0.7555376178519353 1104 15 0.9138995389355244 1104 16 0.920766794456938 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0.81965509514513 1107 17 0.8107918311241065 1107 18 0.8680035443090759 1107 19 0.842697235301415 1107 20 0.8444402851653834 1107 21 0.7926896335275941 1107 22 0.800051092054197 1107 23 0.7755791294833614 1107 24 0.7540804131264096 1107 25 0.7595947105170993 1108 1 0.01809930603953447 1108 2 0.035908622875581386 1108 3 0.01247040405656108 1108 4 0.07362340493798314 1108 5 -0.08556963719847488 1108 6 -0.01788124712581407 1108 7 -0.022422817083182167 1108 8 -0.04126458549963985 1108 9 -0.0022902126966286573 1108 10 0.04893164614729528 1108 11 0.12129192283050827 1108 12 0.09542936384229961 1108 13 -0.08892649025519717 1108 14 0.017825739679572905 1108 15 0.13875421663873055 1108 16 -0.04586564907489077 1108 17 0.06634000095636594 1108 18 0.05203559846894734 1108 19 -0.08112168608828085 1108 20 -0.0315109965988114 1108 21 0.17137206276505879 1108 22 0.3238677850449605 1108 23 0.16780044102113578 1108 24 0.31129238169403095 1108 25 0.38064704745536915 1109 1 -0.023014481786791663 1109 2 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-0.9216412094604394 2315 7 -0.918837296181934 2315 8 -1.005523402516367 2315 9 -1.184001265221248 2315 10 -1.1805153919806202 2315 11 -1.1365627693865719 2315 12 -0.9897887707102555 2315 13 -1.1712456969282883 2315 14 -1.3269604661134977 2315 15 -1.4088413054487532 2315 16 -1.4634696587450335 2315 17 -1.5482076513488465 2315 18 -1.5537663033748714 2315 19 -1.4915429754835827 2315 20 -1.397435927010747 2315 21 -1.6222143868965873 2315 22 -1.6138569910640115 2315 23 -1.5377395228031858 2315 24 -1.2900995174922514 2315 25 -1.299498675970824 2316 1 -1.7382722920028932 2316 2 -1.4601043740410304 2316 3 -1.2512066442051477 2316 4 -0.9979931113705263 2316 5 -1.157572876505597 2316 6 -1.14250739914457 2316 7 -1.1307475434477254 2316 8 -1.0803531382153935 2316 9 -1.3504727327226007 2316 10 -1.6011598727834695 2316 11 -1.2161228303802147 2316 12 -1.548753585193424 2316 13 -1.36606655437987 2316 14 -1.720847788623253 2316 15 -1.700655573666218 2316 16 -1.652173840609791 2316 17 -1.7610457043252765 2316 18 -1.6467154539751263 2316 19 -1.6676273922014744 2316 20 -1.8279276784635101 2316 21 -1.9245704404785045 2316 22 -1.9592743995596393 2316 23 -1.899607243923171 2316 24 -1.61211260445765 2316 25 -1.7401047874117879 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29495.0 3 2.0 4 554.0 5 4637.0 6 5986.0 7 4772.0 8 3992.0 9 3410.0 10 3343.0 11 3142.0 12 2941.0 13 2999.0 14 2873.0 15 2844.0 16 3036.0 17 3234.0 18 3318.0 19 3731.0 20 4559.0 21 6060.0 22 7805.0 23 9558.0 24 11785.0 25 13969.0 26 16247.0 27 18445.0 28 21732.0 29 26672.0 30 33799.0 31 43756.0 32 56540.0 33 74592.0 34 96944.0 35 130551.0 36 193514.0 37 348278.0 38 711906.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.11255356897993 20.577318222726117 13.899538034041422 24.41059017425253 2 14.679221212116456 21.644869417970813 41.124875132193736 22.551034237719 3 18.086980729149495 26.283541625131278 30.867112396985696 24.76236524873353 4 12.39839855239686 18.465155537275624 39.10595435633622 30.030491553991297 5 12.492947027597404 38.58360611805446 36.415791958512294 12.50765489583584 6 30.036456951545798 38.70653436042827 18.72935985528155 12.527648832744381 7 26.595678435768104 32.40838274409334 23.49042736840673 17.505511451731824 8 24.147667660376314 37.696707676158454 21.32713350612055 16.828491157344686 9 26.091863982656392 15.308361341921412 21.27993360617178 37.319841069250415 10 14.637411101186224 29.640909231243402 35.27193055439998 20.449749113170395 11 32.88974846430259 22.903829942214134 25.85617845120488 18.350243142278394 12 22.678923988799703 25.477323378445345 32.67122653283245 19.1725260999225 13 28.360180238731026 22.778137969179827 26.469149213954218 22.392532578134936 14 20.25712956581848 23.28169078204792 28.71878435663449 27.742395295499104 15 22.259200709986963 32.031934761575094 24.11302649212123 21.59583803631671 16 21.099842544736607 28.911172521047675 28.853506951003627 21.1354779832121 17 20.799195822547215 29.41752079124196 28.659130381089483 21.124153005121347 18 20.53919924396356 27.98590699516855 31.105760853241126 20.36913290762677 19 22.664795415644306 27.438735628755516 28.91318305764301 20.983285897957167 20 22.802522839885068 27.865261553902336 29.351011841314147 19.98120376489845 21 22.23178081245575 27.274739524055462 28.459603531306037 22.033876132182744 22 22.417911681765926 28.728098749307307 28.598848468959787 20.25514109996698 23 21.360257160962647 28.445213317907026 29.10564562084875 21.088883900281576 24 22.14166811883841 27.459651866027606 29.79066224787813 20.608017767255856 25 21.879613044545298 28.03595564883902 29.209883093906345 20.874548212709332 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 311.0 1 311.0 2 525.5 3 740.0 4 740.0 5 740.0 6 1813.5 7 2887.0 8 2887.0 9 2887.0 10 4094.0 11 5301.0 12 5301.0 13 5301.0 14 8460.0 15 11619.0 16 11619.0 17 11619.0 18 20172.0 19 28725.0 20 28725.0 21 28725.0 22 45130.0 23 61535.0 24 61535.0 25 61535.0 26 86415.5 27 111296.0 28 111296.0 29 111296.0 30 140257.0 31 169218.0 32 169218.0 33 169218.0 34 188394.0 35 207570.0 36 207570.0 37 207570.0 38 218127.5 39 228685.0 40 228685.0 41 228685.0 42 235406.5 43 242128.0 44 242128.0 45 242128.0 46 242386.5 47 242645.0 48 242645.0 49 242645.0 50 229004.0 51 215363.0 52 215363.0 53 215363.0 54 186475.5 55 157588.0 56 157588.0 57 157588.0 58 131949.5 59 106311.0 60 106311.0 61 106311.0 62 85136.5 63 63962.0 64 63962.0 65 63962.0 66 48252.0 67 32542.0 68 32542.0 69 32542.0 70 23559.0 71 14576.0 72 14576.0 73 14576.0 74 9998.5 75 5421.0 76 5421.0 77 5421.0 78 3637.5 79 1854.0 80 1854.0 81 1854.0 82 1212.0 83 570.0 84 570.0 85 570.0 86 352.5 87 135.0 88 135.0 89 135.0 90 85.0 91 35.0 92 35.0 93 35.0 94 19.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39947232395667026 2 0.0 3 0.0 4 0.0 5 0.025065135338648816 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0465609744738546E-4 17 0.0 18 1.0465609744738546E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004709524385132346 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1911021.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.12485832955225 #Duplication Level Percentage of deduplicated Percentage of total 1 86.15442681882976 64.72339109228365 2 10.155749513043709 15.25898486795656 3 2.090419310130223 4.711273635686801 4 0.6611852308943214 1.9868578720211278 5 0.274904911720817 1.0326096273562224 6 0.1620962025049969 0.7306472549367784 7 0.0948848147502857 0.49897457860187167 8 0.07003325353496444 0.42089906001374544 9 0.053357955802840785 0.3607657983398632 >10 0.24555837550715898 3.3341785810165234 >50 0.01882767527195855 0.9849337794155123 >100 0.015151613999342683 2.2870546828978306 >500 0.0020842800059087878 1.1409553033211757 >1k 0.0011810920033483087 1.8873780751782674 >5k 1.3895200039391867E-4 0.6410957909741063 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7127 0.37294200325375804 No Hit GGTATCAACGCAGAGTACTTTTTTT 5156 0.26980341921935974 No Hit TATCAACGCAGAGTACTTTTTTTTT 4873 0.25499458143055465 No Hit GTCCTACAGTGGACATTTCTAAATT 3614 0.18911356808742552 No Hit CTGTAGGACGTGGAATATGGCAAGA 3582 0.18743907052826736 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3256 0.17038012664434352 No Hit CTTTAGGACGTGAAATATGGCGAGG 3251 0.17011848640072505 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2312 0.12098244864917759 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.232804872369273E-5 0.0 3 0.0 0.0 0.0 5.232804872369273E-5 0.0 4 0.0 0.0 0.0 1.5698414617107818E-4 0.0 5 0.0 0.0 0.0 1.5698414617107818E-4 0.0 6 0.0 0.0 0.0 1.5698414617107818E-4 0.0 7 5.232804872369273E-5 0.0 0.0 1.5698414617107818E-4 1.0465609744738546E-4 8 5.232804872369273E-5 0.0 0.0 1.5698414617107818E-4 1.0465609744738546E-4 9 5.232804872369273E-5 0.0 0.0 2.093121948947709E-4 1.0465609744738546E-4 10 5.232804872369273E-5 0.0 0.0 2.093121948947709E-4 1.0465609744738546E-4 11 5.232804872369273E-5 0.0 0.0 2.616402436184636E-4 1.0465609744738546E-4 12 5.232804872369273E-5 0.0 0.0 3.1396829234215637E-4 1.0465609744738546E-4 13 5.232804872369273E-5 0.0 0.0 3.6629634106584907E-4 1.5698414617107818E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGAGG 75 5.8631485E-8 15.196754 19 GGTATCA 1485 0.0 13.805821 1 TAGGACC 570 0.0 12.833953 4 TATGTCG 115 5.349648E-9 12.387687 16 TGTCGAG 125 1.8380888E-8 11.397566 18 GGCGAGG 900 0.0 10.869902 19 CAAGTCG 180 3.6379788E-11 10.552474 6 AAGTCGT 210 0.0 10.401724 7 TGGTATC 360 0.0 10.292163 2 GACGTGG 1070 0.0 10.207299 7 TGTCCAC 700 0.0 10.039925 10 GTATAAG 210 5.456968E-12 9.989727 1 GGACGTG 2230 0.0 9.923111 6 AGGACCT 1275 0.0 9.910282 5 GTGGTAT 405 0.0 9.888822 1 AATGTCC 635 0.0 9.871133 8 AGGACGT 2320 0.0 9.869011 5 GTCCACT 750 0.0 9.750486 11 GTAGGAC 2075 0.0 9.6607065 3 GTATCAA 2870 0.0 9.568873 1 >>END_MODULE