FastQCFastQC Report
Thu 26 May 2016
SRR1511852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552385
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78130.30610585785451644No Hit
CTGTAGGACGTGGAATATGGCAAGA70350.2756245629088088No Hit
GTATCAACGCAGAGTACTTTTTTTT69390.2718633748435287No Hit
GTCCTAAAGTGTGTATTTCTCATTT66290.25971787171606164No Hit
CTTTAGGACGTGAAATATGGCGAGG59090.23150896122646072No Hit
GGTATCAACGCAGAGTACTTTTTTT44180.17309300908757888No Hit
TATCAACGCAGAGTACTTTTTTTTT41680.16329824850091187No Hit
GTCCTACAGTGTGCATTTCTCATTT38580.15115274537344484No Hit
CTGAAGGACCTGGAATATGGCGAGA33260.1303094948450175No Hit
CTGTAGGACCTGGAATATGGCGAGA32850.12870315410880412No Hit
ATTTAGAAATGTCCACTGTAGGACG26380.10335431371050997No Hit
TTTCTAAATTTTCCACCTTTTTCAG26200.10264909094826996No Hit
GTCCTTCAGTGTGCATTTCTCATTT26130.10237483765184328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13200.013.7380564
TCCAACG2900.013.6788418
CGGTAGG1101.2860255E-913.6302721
CCAACGA1650.013.16563719
GGTATCA12850.012.9125171
ATACGGC1052.1644155E-812.59321815
GGCGAGG17700.012.16632919
GGACCTG25500.011.1857416
AAATGTC10150.011.1664017
AGGACCT27100.011.0829615
CACCTTT10000.010.956099514
GTATCAA26900.010.813011
CGTGATA700.001566114310.7941879
CCACCTT10200.010.74127513
AATGTCC10300.010.7286878
GAAATGT10200.010.556086
ATGTCGA2250.010.49434917
ATCCCGT2250.010.49434910
ACTGTTC7400.010.4659858
TAGAAAT12450.010.3931984
Produced by FastQC (version 0.11.5)