FastQCFastQC Report
Thu 26 May 2016
SRR1511852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552385
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87230.3417587863899843No Hit
GTCCTACAGTGGACATTTCTAAATT84510.3311020868716906No Hit
CTGTAGGACGTGGAATATGGCAAGA78640.3081039890141965No Hit
GTCCTAAAGTGTGTATTTCTCATTT70890.2777402311955289No Hit
CTTTAGGACGTGAAATATGGCGAGG67240.2634398807389951No Hit
TATCAACGCAGAGTACTTTTTTTTT59170.23182239356523407No Hit
GGTATCAACGCAGAGTACTTTTTTT58620.22966754623616734No Hit
GTCCTACAGTGTGCATTTCTCATTT42590.16686354135445866No Hit
CTGAAGGACCTGGAATATGGCGAGA38200.14966394176427145No Hit
CTGTAGGACCTGGAATATGGCGAGA36350.14241581893013788No Hit
ATTTAGAAATGTCCACTGTAGGACG29590.11593078630379038No Hit
TTTCTAAATTTTCCACCTTTTTCAG29110.11405019227115033No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27690.10848676825792347No Hit
GTCCTTCAGTGTGCATTTCTCATTT27580.10805579879211012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.013.6975531
TAGGACC15950.013.1032184
ACCTTTT11500.011.31431115
ACTGTTC8100.011.0216918
GATATAC7200.010.9932011
CCACCTT11700.010.8773813
TGTAGGA47300.010.724972
CACCTTT12050.010.71907414
CTGTAGG46000.010.61428451
GGACCTG30150.010.5526826
GGCGAGG17750.010.48769719
GTAGGAC46900.010.4518423
AGGACCT31300.010.3496585
CCAACGA1752.3646862E-1010.31188219
GGCGAAA1201.5292335E-610.289268519
GGACGTG44100.010.2727136
AGGACGT44950.010.2289645
GACGTGA24800.010.0335587
TCCAACG2400.09.89352718
GACGTGG22100.09.8841877
Produced by FastQC (version 0.11.5)