FastQCFastQC Report
Thu 26 May 2016
SRR1511851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177324
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63900.2934795188956719No Hit
GGTATCAACGCAGAGTACTTTTTTT42550.19542337291096776No Hit
TATCAACGCAGAGTACTTTTTTTTT41810.1920247055559944No Hit
GTCCTACAGTGGACATTTCTAAATT23710.1088951391708354No Hit
GTCCTAAAGTGTGTATTTCTCATTT22340.10260301177041176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.013.3243931
CGGTAGG706.797385E-512.8485211
ACGAAAT1403.6379788E-1212.81827712
GGACCGT701.1519993E-412.1436316
AGGACCG1052.9343028E-711.6938675
TAGTACC1800.011.5439464
TAGGACC6300.011.5439454
TGGTATC4550.010.9816422
GGCGAGG7800.010.89863219
GTAGTAC1658.913048E-1110.8761153
ACGGTAC700.001565770410.7943393
GTGGTAT5050.010.6858991
TGGCGAG16350.010.28315318
GTATCAA28550.010.25581551
GTCCTAC14200.010.1340451
AGGACGG750.002778825610.07471755
TATACTG3000.010.07471755
TGTAGGA14300.09.7836452
CGAAATC1857.203198E-109.70031713
TAAGACA3850.09.567714
Produced by FastQC (version 0.11.5)