FastQCFastQC Report
Thu 26 May 2016
SRR1511851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177324
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85630.3932809264950921No Hit
GGTATCAACGCAGAGTACTTTTTTT57560.26436120669225155No Hit
TATCAACGCAGAGTACTTTTTTTTT56450.25926320565979155No Hit
GTCCTACAGTGGACATTTCTAAATT25570.11743773549549816No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25510.11716216787212193No Hit
GTCCTAAAGTGTGTATTTCTCATTT25440.11684067231151633No Hit
CTGTAGGACGTGGAATATGGCAAGA23960.11004333760156963No Hit
CTTTAGGACGTGAAATATGGCGAGG22930.10531276006694457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.014.3615511
TAGGACC5400.013.017484
CGAACCG751.48324125E-512.66332812
GCGAACC751.48324125E-512.66332811
AATACCG550.003070959612.0899455
GGCGAAC907.486222E-611.60805110
TTAGACT1354.7366484E-911.2583614
GTAGGAC17050.010.6970953
TGTAGGA17300.010.5425122
GACGTGG8050.010.2643757
CTGTAGG17400.010.2508651
AGGACGT15150.010.2203065
ACCGTCC750.002654096810.1306638
TAGACTG2350.010.10557655
GGACGTG15150.09.967676
TAGGACA6300.09.9515874
GTCCTAC15300.09.9122861
TAGGACG16200.09.9097024
GGCGAGG6250.09.87739719
CTTAGAC1451.6195372E-79.8268013
Produced by FastQC (version 0.11.5)