##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511851_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2177324 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.469161227267968 33.0 31.0 34.0 28.0 34.0 2 31.657057929825786 33.0 31.0 34.0 28.0 34.0 3 31.77393810016332 34.0 31.0 34.0 28.0 34.0 4 35.33164425689516 37.0 35.0 37.0 33.0 37.0 5 35.044292902664004 37.0 35.0 37.0 32.0 37.0 6 35.010382928769445 37.0 35.0 37.0 32.0 37.0 7 34.87884807222076 37.0 35.0 37.0 32.0 37.0 8 34.812958934912764 37.0 35.0 37.0 32.0 37.0 9 36.41197221910933 39.0 37.0 39.0 32.0 39.0 10 36.26109067828215 39.0 37.0 39.0 32.0 39.0 11 36.244586933318146 39.0 37.0 39.0 32.0 39.0 12 36.11104364807443 39.0 37.0 39.0 32.0 39.0 13 36.10050180864217 39.0 37.0 39.0 32.0 39.0 14 37.2549946631737 40.0 37.0 41.0 32.0 41.0 15 37.191324304513245 40.0 37.0 41.0 31.0 41.0 16 37.11166872730012 40.0 37.0 41.0 31.0 41.0 17 37.07174816426035 40.0 37.0 41.0 31.0 41.0 18 37.01565913019835 40.0 37.0 41.0 31.0 41.0 19 37.03859003069823 40.0 37.0 41.0 31.0 41.0 20 36.9190028677404 40.0 37.0 41.0 31.0 41.0 21 36.829876490591204 40.0 37.0 41.0 31.0 41.0 22 36.91170997058775 40.0 37.0 41.0 31.0 41.0 23 36.82505084222651 40.0 37.0 41.0 31.0 41.0 24 36.733859545019484 40.0 37.0 41.0 31.0 41.0 25 36.33991220415519 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19970471949130086 1101 2 0.22752439294618299 1101 3 0.3010316157143329 1101 4 0.1455558902667633 1101 5 0.28935706069083267 1101 6 0.25050745243798644 1101 7 0.25623882518892316 1101 8 0.2127014663182223 1101 9 0.14374456295008287 1101 10 0.2349201217659811 1101 11 0.13593953509294465 1101 12 0.23707016283496785 1101 13 0.29042081209328074 1101 14 0.14593236866684833 1101 15 0.11408869871421956 1101 16 0.14541115222047551 1101 17 0.20502764148402974 1101 18 0.15319488492076516 1101 19 0.24909917111418167 1101 20 0.21648167123578332 1101 21 0.22754142354840212 1101 22 0.023079685284976392 1101 23 -0.17226989515143742 1101 24 -0.1718212475193397 1101 25 0.04131774875850169 1102 1 0.3891004378424192 1102 2 0.4147916574519357 1102 3 0.39845771099090044 1102 4 0.26963267991136775 1102 5 0.3328293004745504 1102 6 0.3605299329830345 1102 7 0.35401770135872823 1102 8 0.47639081550999407 1102 9 0.33134326086289434 1102 10 0.5224343602655708 1102 11 0.34433998984913927 1102 12 0.4299033738307827 1102 13 0.5301391238027051 1102 14 0.7378064754250744 1102 15 0.788725914607916 1102 16 0.8107958749741044 1102 17 0.8891636676659047 1102 18 0.8067585740510097 1102 19 0.8125263841232311 1102 20 0.8696405791859689 1102 21 0.9441677763084968 1102 22 0.8424364362632701 1102 23 0.8525941788356661 1102 24 0.6338520385101489 1102 25 0.8568743735129374 1103 1 0.28999344755022705 1103 2 0.35928143729225326 1103 3 0.32105927780347443 1103 4 0.3390434776848039 1103 5 0.41754012896250003 1103 6 0.4031802490976091 1103 7 0.3997980228869338 1103 8 0.35420695537226976 1103 9 0.475018227899362 1103 10 0.4890537574627274 1103 11 0.44520468046034267 1103 12 0.5637608766081286 1103 13 0.3884672537681908 1103 14 0.5620037062219865 1103 15 0.6083122428720245 1103 16 0.40078592602832686 1103 17 0.40223223873858416 1103 18 0.7300393733308965 1103 19 0.7446246961027967 1103 20 0.6254365454181041 1103 21 0.5438565856342095 1103 22 0.6510405975735267 1103 23 0.6097832104024121 1103 24 0.6581500363180837 1103 25 0.5691496904925231 1104 1 0.270230936451874 1104 2 0.25651861769078366 1104 3 0.34501697345561766 1104 4 0.20780248893868247 1104 5 0.28849350531671547 1104 6 0.3959697200109602 1104 7 0.31315188409715944 1104 8 0.4086009120684011 1104 9 0.40475378248902416 1104 10 0.2710071645909551 1104 11 0.4989526372312483 1104 12 0.40879058115981337 1104 13 0.46966791838512734 1104 14 0.6219804756577503 1104 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2314 17 -0.46574030462493 2314 18 -0.4518305412596888 2314 19 -0.620944684841092 2314 20 -0.4157098781654298 2314 21 -0.5392628417503644 2314 22 -0.48131795584719583 2314 23 -0.528950795541455 2314 24 -0.6000721520845076 2314 25 -0.6520897616061916 2315 1 -0.5530379224869577 2315 2 -0.41944862031601104 2315 3 -0.5555758919490579 2315 4 -0.4910318780210332 2315 5 -0.5427577367066334 2315 6 -0.6340909076329808 2315 7 -0.7443309285536941 2315 8 -0.7055559189003446 2315 9 -0.6801179693264388 2315 10 -0.7771651217500377 2315 11 -0.854957806358442 2315 12 -0.8070237710515258 2315 13 -0.8079376822432778 2315 14 -0.9400395492334326 2315 15 -0.9469746734317397 2315 16 -0.9767691974045434 2315 17 -0.9937060507685374 2315 18 -1.0532193479258254 2315 19 -1.0544098891725042 2315 20 -0.9606841517381213 2315 21 -1.1402157963213568 2315 22 -1.2100755285628537 2315 23 -1.0939562479825256 2315 24 -1.1570023290936504 2315 25 -1.198068296748481 2316 1 -1.3940733986022735 2316 2 -0.9556960418502456 2316 3 -0.7526194591928075 2316 4 -0.5892851438413231 2316 5 -0.5451574505901675 2316 6 -0.5914998859833673 2316 7 -0.6682478594838557 2316 8 -0.7584366958619597 2316 9 -0.9146385952090341 2316 10 -0.9574752154951582 2316 11 -0.7293765613944814 2316 12 -0.7784912552262071 2316 13 -0.9135964236995591 2316 14 -0.939390058062969 2316 15 -0.9871408807748452 2316 16 -1.030729717984478 2316 17 -1.130694374392121 2316 18 -1.1455499065988448 2316 19 -0.8904133686300071 2316 20 -1.0458827868423555 2316 21 -1.2414075133519518 2316 22 -1.132960789566127 2316 23 -1.071038143713949 2316 24 -0.8931442721273228 2316 25 -1.0266292880818852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19531.0 3 0.0 4 361.0 5 3180.0 6 4317.0 7 3520.0 8 2917.0 9 2584.0 10 2426.0 11 2389.0 12 2368.0 13 2288.0 14 2327.0 15 2229.0 16 2399.0 17 2529.0 18 2776.0 19 3136.0 20 3787.0 21 4834.0 22 6283.0 23 8022.0 24 10237.0 25 12138.0 26 14612.0 27 16734.0 28 19641.0 29 24402.0 30 31435.0 31 41506.0 32 54507.0 33 72254.0 34 96477.0 35 130671.0 36 193922.0 37 351372.0 38 1023213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.493876816774986 19.33989547922269 13.480827863597156 24.685399840405168 2 15.097661165724533 20.833601246300503 40.34181408003586 23.726923507939105 3 18.349221337752216 25.299082727237654 30.155824305431807 26.195871629578328 4 12.628896755834226 17.532944109374625 38.17470436186806 31.663454772923096 5 13.267076739028866 37.81440527333027 35.55482661969624 13.363691367944616 6 31.201419724395635 37.46011158651629 18.22778787171776 13.110680817370312 7 27.49099353150932 31.873804725433608 22.578633221330406 18.05656852172667 8 25.167177691514908 36.34181224291837 20.780324839114435 17.710685226452288 9 26.464182638872302 15.125906847120593 20.38653870530982 38.023371808697284 10 15.200126393683256 29.02677782452221 34.26398643472446 21.509109347070073 11 33.79768008803467 22.68546160332592 24.60506566776465 18.91179264087476 12 22.952348846565783 24.981996248606087 31.76422066720433 20.3014342376238 13 28.713641148492368 22.02143548686369 26.09813697915423 23.16678638548971 14 21.207225015661425 22.1271615983657 27.95927477949997 28.706338606472897 15 23.122833349561205 30.371915250095988 23.882022151962683 22.623229248380124 16 22.244185982426135 28.229468834220356 27.425270653334096 22.101074530019417 17 21.697138322087113 28.412216096456017 27.782222581480752 22.108422999976117 18 21.52859268009386 27.397496834415474 29.647277872874167 21.4266326126165 19 23.298415853589084 26.868118846804613 28.05416189781585 21.779303401790454 20 23.442399936803156 27.04154273778271 28.445881274445146 21.07017605096899 21 23.24564465371254 26.418208773705704 27.702124258952733 22.634022313629025 22 23.146853660732162 27.42954195149642 27.821582823686324 21.60202156408509 23 22.576290896531706 27.20771001467857 28.059213970911085 22.156785117878645 24 22.868583428179313 26.65429305525318 28.595489464976804 21.881634051590705 25 22.707298231497226 27.063015943438774 28.26513487669715 21.96455094836685 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 358.0 1 358.0 2 555.5 3 753.0 4 753.0 5 753.0 6 1918.0 7 3083.0 8 3083.0 9 3083.0 10 4236.0 11 5389.0 12 5389.0 13 5389.0 14 8213.0 15 11037.0 16 11037.0 17 11037.0 18 19348.0 19 27659.0 20 27659.0 21 27659.0 22 42658.0 23 57657.0 24 57657.0 25 57657.0 26 81918.0 27 106179.0 28 106179.0 29 106179.0 30 134608.0 31 163037.0 32 163037.0 33 163037.0 34 184553.5 35 206070.0 36 206070.0 37 206070.0 38 222091.0 39 238112.0 40 238112.0 41 238112.0 42 251051.0 43 263990.0 44 263990.0 45 263990.0 46 271530.0 47 279070.0 48 279070.0 49 279070.0 50 272310.5 51 265551.0 52 265551.0 53 265551.0 54 237618.0 55 209685.0 56 209685.0 57 209685.0 58 180749.5 59 151814.0 60 151814.0 61 151814.0 62 124100.0 63 96386.0 64 96386.0 65 96386.0 66 74416.5 67 52447.0 68 52447.0 69 52447.0 70 38424.5 71 24402.0 72 24402.0 73 24402.0 74 17084.0 75 9766.0 76 9766.0 77 9766.0 78 6578.5 79 3391.0 80 3391.0 81 3391.0 82 2249.0 83 1107.0 84 1107.0 85 1107.0 86 702.0 87 297.0 88 297.0 89 297.0 90 182.0 91 67.0 92 67.0 93 67.0 94 40.0 95 13.0 96 13.0 97 13.0 98 8.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4286913660989361 2 0.0 3 0.0 4 0.0 5 0.02916424014064971 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.592793722936963E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005052073095230659 25 9.185587445873926E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2177324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.55064960367864 #Duplication Level Percentage of deduplicated Percentage of total 1 80.91727870200354 56.27849297886256 2 12.726486180840324 17.702707620993674 3 3.46941307294038 7.238997988994949 4 1.2254967539610573 3.4093638130076425 5 0.560658826296986 1.94970927874957 6 0.307856791868202 1.284698391560276 7 0.1835156506310581 0.8934542899682281 8 0.12967315081845102 0.7215081500463247 9 0.08314906161308605 0.5204764124213791 >10 0.3599209259424642 4.238370105604126 >50 0.019250693261636993 0.9104382698812965 >100 0.014595399217699897 2.0221375672899815 >500 0.001517321559003782 0.7299053812930689 >1k 9.895575384805554E-4 1.1837332745774043 >5k 1.979115076961111E-4 0.9160064767496142 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8563 0.3932809264950921 No Hit GGTATCAACGCAGAGTACTTTTTTT 5756 0.26436120669225155 No Hit TATCAACGCAGAGTACTTTTTTTTT 5645 0.25926320565979155 No Hit GTCCTACAGTGGACATTTCTAAATT 2557 0.11743773549549816 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2551 0.11716216787212193 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2544 0.11684067231151633 No Hit CTGTAGGACGTGGAATATGGCAAGA 2396 0.11004333760156963 No Hit CTTTAGGACGTGAAATATGGCGAGG 2293 0.10531276006694457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 4.592793722936963E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1760 0.0 14.361551 1 TAGGACC 540 0.0 13.01748 4 CGAACCG 75 1.48324125E-5 12.663328 12 GCGAACC 75 1.48324125E-5 12.663328 11 AATACCG 55 0.0030709596 12.089945 5 GGCGAAC 90 7.486222E-6 11.608051 10 TTAGACT 135 4.7366484E-9 11.258361 4 GTAGGAC 1705 0.0 10.697095 3 TGTAGGA 1730 0.0 10.542512 2 GACGTGG 805 0.0 10.264375 7 CTGTAGG 1740 0.0 10.250865 1 AGGACGT 1515 0.0 10.220306 5 ACCGTCC 75 0.0026540968 10.130663 8 TAGACTG 235 0.0 10.1055765 5 GGACGTG 1515 0.0 9.96767 6 TAGGACA 630 0.0 9.951587 4 GTCCTAC 1530 0.0 9.912286 1 TAGGACG 1620 0.0 9.909702 4 GGCGAGG 625 0.0 9.877397 19 CTTAGAC 145 1.6195372E-7 9.826801 3 >>END_MODULE