FastQCFastQC Report
Thu 26 May 2016
SRR1511850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802769
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33440.4165581879718824No Hit
GTCCTACAGTGGACATTTCTAAATT31660.39438493514323547No Hit
CTGTAGGACGTGGAATATGGCAAGA28220.3515332555193337No Hit
GTCCTAAAGTGTGTATTTCTCATTT28080.34978929181370977No Hit
CTTTAGGACGTGAAATATGGCGAGG22600.2815255696221453No Hit
GGTATCAACGCAGAGTACTTTTTTT20860.25985059213796247No Hit
TATCAACGCAGAGTACTTTTTTTTT20240.2521273242987709No Hit
GTCCTACAGTGTGCATTTCTCATTT16210.20192608334402548No Hit
CTGAAGGACCTGGAATATGGCGAGA12420.15471449445606394No Hit
CTGTAGGACCTGGAATATGGCGAGA11500.14325416153339254No Hit
TTTCTAAATTTTCCACCTTTTTCAG10210.12718478167442937No Hit
GAATATGGCAAGAAAACTGAAAATC10120.12606366214938544No Hit
ATTTAGAAATGTCCACTGTAGGACG10080.12556538680492146No Hit
GTCCTTCAGTGTGCATTTCTCATTT10040.1250671114604575No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9480.11809125663796184No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9070.1129839343572061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4950.014.5014281
TAAGGCC400.005473735714.171244
CGGTAGG500.001040967413.9576241
GTATACT751.0278491E-613.8563234
GTATAAG706.9381604E-512.8182271
TAGGACC4800.012.2030114
ATGTCGA701.1468113E-412.14753317
GTGGTAT1153.6056008E-812.1370641
GTCGAGG550.003194659212.02483119
AACAACC550.003196149612.0240817
CTCTAGG1352.2064341E-911.8159791
TATGTCG658.3930296E-411.62840816
TGAACAG752.180826E-411.3369915
TAGGACT1355.136826E-911.1970284
GGCGAGG7900.011.12242619
TGTAGGA15100.011.0261282
GCGCCAC600.006118688711.02207513
CGCCACC600.006118688711.02207514
AGGACCT8800.010.8431445
CTGTAGG15450.010.8408731
Produced by FastQC (version 0.11.5)