##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511850_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 802769 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.30596223820302 31.0 30.0 34.0 26.0 34.0 2 27.574168907867644 31.0 28.0 34.0 10.0 34.0 3 29.34664268301342 31.0 30.0 34.0 16.0 34.0 4 33.77451794974644 35.0 35.0 37.0 28.0 37.0 5 34.14031059993597 37.0 35.0 37.0 31.0 37.0 6 34.357092762675194 37.0 35.0 37.0 32.0 37.0 7 34.375849092329176 37.0 35.0 37.0 32.0 37.0 8 34.3951622446806 37.0 35.0 37.0 32.0 37.0 9 36.0335924780354 39.0 37.0 39.0 32.0 39.0 10 35.9175005512171 39.0 37.0 39.0 32.0 39.0 11 35.92472554371183 39.0 37.0 39.0 32.0 39.0 12 35.84209405196265 39.0 37.0 39.0 31.0 39.0 13 35.8429199433461 39.0 37.0 39.0 31.0 39.0 14 37.030506907964806 40.0 38.0 41.0 31.0 41.0 15 37.00091433525709 40.0 38.0 41.0 31.0 41.0 16 36.96156303992805 40.0 37.0 41.0 31.0 41.0 17 36.93396855135163 40.0 38.0 41.0 31.0 41.0 18 36.918801049866154 40.0 38.0 41.0 31.0 41.0 19 36.9579630005643 40.0 38.0 41.0 31.0 41.0 20 36.92149049103789 40.0 38.0 41.0 31.0 41.0 21 36.811884863516156 40.0 37.0 41.0 31.0 41.0 22 36.750650560746614 40.0 37.0 41.0 31.0 41.0 23 36.651557297304706 40.0 37.0 41.0 31.0 41.0 24 36.580984068891546 40.0 37.0 41.0 31.0 41.0 25 36.49504029179004 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0579228871738344 1101 2 3.6795553928641844 1101 3 2.1710655428013297 1101 4 1.2580291959888044 1101 5 0.6331289599109695 1101 6 0.6492800868507587 1101 7 0.413907920418616 1101 8 0.4740952704245629 1101 9 0.4167733263924944 1101 10 0.4750002960946702 1101 11 0.5221047521319093 1101 12 0.44735912449418436 1101 13 0.3650165740394087 1101 14 0.410600963790948 1101 15 0.5208153580783659 1101 16 0.6601530676198593 1101 17 0.3929010916278344 1101 18 0.6709358529638081 1101 19 0.6090530083375398 1101 20 0.3885301314268972 1101 21 0.5473951619138191 1101 22 0.3699667071050925 1101 23 0.13850127967049986 1101 24 0.2204045060106523 1101 25 0.2109686210652768 1102 1 0.9715818570298431 1102 2 3.896882041033816 1102 3 2.1359568606716053 1102 4 1.074982335228647 1102 5 0.8511253781599635 1102 6 0.6564667342681645 1102 7 0.6113497748282484 1102 8 0.38601833510728056 1102 9 0.46354584469906257 1102 10 0.48495281908817844 1102 11 0.42571861738516503 1102 12 0.4377234041947986 1102 13 0.5924187051523262 1102 14 0.5751329492903565 1102 15 0.5863530113081623 1102 16 0.6510873028523676 1102 17 0.6240874960185181 1102 18 0.6246855527968691 1102 19 0.7607118103893811 1102 20 0.7181365727923605 1102 21 0.8563891759341473 1102 22 0.8422037970336618 1102 23 0.8770855332746734 1102 24 0.6617121055141837 1102 25 0.7153069374108512 1103 1 1.4209618931707233 1103 2 4.1217879951871765 1103 3 2.5176466173457364 1103 4 1.7259671174173334 1103 5 1.3941961029096461 1103 6 1.3550654227405872 1103 7 1.375669746642906 1103 8 1.1487699290565274 1103 9 1.111905298721723 1103 10 1.3228984713856278 1103 11 1.42815642211076 1103 12 1.1203655526451755 1103 13 1.1721531037703343 1103 14 1.2972516232155087 1103 15 1.0131109014989832 1103 16 1.1126194261859723 1103 17 1.0528967789970736 1103 18 1.2830831934110378 1103 19 1.053086425398078 1103 20 1.3112305959088886 1103 21 1.4605641296215381 1103 22 1.41014410775432 1103 23 1.3463686254793785 1103 24 1.4830335206910803 1103 25 1.3234539785182662 1104 1 1.5984138298442971 1104 2 4.211171930406298 1104 3 2.4890337154232043 1104 4 1.7906845090980923 1104 5 1.3927604076291118 1104 6 1.14870045889991 1104 7 1.0081389693762404 1104 8 1.0326979336276665 1104 9 1.1891933208386476 1104 10 1.0645252656440007 1104 11 1.1463647898334415 1104 12 1.1695197189030182 1104 13 1.2000892809827448 1104 14 1.236167478485882 1104 15 1.1378215980781334 1104 16 1.1762041336504296 1104 17 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-1.0703490409033378 2315 16 -1.1158324661471895 2315 17 -0.9922176226900419 2315 18 -1.0460966065844275 2315 19 -1.1095193026229637 2315 20 -0.9421424964514813 2315 21 -1.164183597084282 2315 22 -1.122566384547092 2315 23 -1.2213823439303582 2315 24 -1.3460385671113642 2315 25 -1.2331175990685281 2316 1 -1.4379714712825375 2316 2 -1.1725345628709398 2316 3 -0.9246045204766489 2316 4 -1.1797103245245495 2316 5 -1.3390866605399978 2316 6 -1.1995033717511845 2316 7 -1.2582898752499645 2316 8 -1.4016793649100947 2316 9 -1.5009608466861408 2316 10 -1.497669856307219 2316 11 -1.7724854264129917 2316 12 -1.4657004994029066 2316 13 -1.7017936811520613 2316 14 -1.8498250485081513 2316 15 -2.373620339019162 2316 16 -2.075640580816014 2316 17 -1.838379916066529 2316 18 -1.8002637548909775 2316 19 -1.57681788082202 2316 20 -1.7391428432322016 2316 21 -1.9826381110133084 2316 22 -2.0505689276056955 2316 23 -1.97757815944302 2316 24 -1.8834619863692552 2316 25 -2.0003352617664873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20831.0 3 0.0 4 200.0 5 938.0 6 1170.0 7 972.0 8 926.0 9 844.0 10 795.0 11 781.0 12 743.0 13 741.0 14 801.0 15 809.0 16 825.0 17 844.0 18 995.0 19 1280.0 20 1671.0 21 1874.0 22 2567.0 23 2903.0 24 3371.0 25 3922.0 26 4671.0 27 5113.0 28 6282.0 29 7570.0 30 9862.0 31 13263.0 32 17925.0 33 25715.0 34 37675.0 35 58548.0 36 97046.0 37 140031.0 38 328265.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.97762743703357 19.701807120105535 13.559442380062011 26.761123062798887 2 14.174558437665963 20.99977915310474 42.92540633199598 21.900256077233315 3 17.23634071569779 25.28971597059677 30.908144185936425 26.565799127769008 4 11.054861361113845 17.334750096229428 40.088742838849036 31.521645703807692 5 11.386342119687079 38.43303104290199 37.4246350092182 12.755991828192734 6 28.903441351527093 39.00264834335286 19.19675222916765 12.897158075952394 7 25.603629437609076 32.00733959582395 24.12686588545397 18.262165081112997 8 22.049306836711434 39.58274422654587 21.801539421676722 16.566409515065978 9 24.81062422689466 15.06211625012924 22.116698577050183 38.01056094592592 10 14.005647951029498 29.09143228002078 36.25825112828223 20.64466864066749 11 31.37104198094346 22.178360150927602 27.896069728651707 18.554528139477235 12 22.863613318401683 24.616296842553712 33.832771320267724 18.687318518776884 13 27.730213125352687 23.173349178528763 27.027892277584904 22.068545418533645 14 18.465585990490414 24.151281377332705 29.063279723058567 28.319852909118314 15 20.284415566620037 34.46720040260648 24.213316657718472 21.035067373055014 16 18.99213846075272 28.679732276657422 31.80229929157703 20.525829971012833 17 19.036805245862432 30.19497587092627 30.324901652536358 20.443317230674943 18 18.858226961928025 27.29203544232525 34.26191096068732 19.5878266350594 19 21.743544515407333 26.868692908403062 29.596881785125696 21.79088079106391 20 21.778949282854533 28.007912642040147 30.804618033150383 19.40852004195494 21 20.06313172207094 27.350311298684797 29.232927154363786 23.353629824880475 22 20.943805443680247 30.105801584345404 29.452903781973745 19.49748919000061 23 18.976341857174177 29.601578538257627 30.87168397345186 20.550395631116338 24 21.417110767291273 27.045665752305215 31.799722452681173 19.73750102772234 25 19.96656522459474 28.54799148434707 31.082257769163025 20.403185521895168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 166.0 1 166.0 2 265.0 3 364.0 4 364.0 5 364.0 6 943.5 7 1523.0 8 1523.0 9 1523.0 10 1969.0 11 2415.0 12 2415.0 13 2415.0 14 3736.0 15 5057.0 16 5057.0 17 5057.0 18 9009.5 19 12962.0 20 12962.0 21 12962.0 22 20517.5 23 28073.0 24 28073.0 25 28073.0 26 40755.5 27 53438.0 28 53438.0 29 53438.0 30 68460.5 31 83483.0 32 83483.0 33 83483.0 34 90545.0 35 97607.0 36 97607.0 37 97607.0 38 99503.5 39 101400.0 40 101400.0 41 101400.0 42 101840.5 43 102281.0 44 102281.0 45 102281.0 46 100339.0 47 98397.0 48 98397.0 49 98397.0 50 90595.0 51 82793.0 52 82793.0 53 82793.0 54 69195.5 55 55598.0 56 55598.0 57 55598.0 58 46350.0 59 37102.0 60 37102.0 61 37102.0 62 29545.5 63 21989.0 64 21989.0 65 21989.0 66 16391.0 67 10793.0 68 10793.0 69 10793.0 70 7702.0 71 4611.0 72 4611.0 73 4611.0 74 3211.5 75 1812.0 76 1812.0 77 1812.0 78 1226.0 79 640.0 80 640.0 81 640.0 82 406.5 83 173.0 84 173.0 85 173.0 86 123.0 87 73.0 88 73.0 89 73.0 90 42.5 91 12.0 92 12.0 93 12.0 94 8.5 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.239489815874803 3 0.0 4 0.0 5 0.0011211195250439415 6 3.7370650834798053E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.49137672231987E-4 14 0.0 15 0.0 16 0.0 17 3.7370650834798053E-4 18 0.0 19 2.49137672231987E-4 20 3.7370650834798053E-4 21 3.7370650834798053E-4 22 0.00498275344463974 23 1.245688361159935E-4 24 0.0028650832306678507 25 0.0014948260333919221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 802769.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.97633900967541 #Duplication Level Percentage of deduplicated Percentage of total 1 89.5003871804888 67.10411370741537 2 7.502112056895889 11.249617937327988 3 1.519214284127477 3.4171537558524916 4 0.5131170854469514 1.5388656220050887 5 0.24492890486163357 0.9181936302087187 6 0.14302601934410172 0.643414038812866 7 0.09853616606067828 0.517151669389536 8 0.07890528521904162 0.4732823531390396 9 0.052360379716780626 0.3533210622288608 >10 0.28977364436372566 4.016995616864376 >50 0.029581006803787533 1.5475509329626613 >100 0.02248019416540613 3.305066738895776 >500 0.00321145629126091 1.548362932980322 >1k 0.0023663362146133025 3.3669100019169416 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3344 0.4165581879718824 No Hit GTCCTACAGTGGACATTTCTAAATT 3166 0.39438493514323547 No Hit CTGTAGGACGTGGAATATGGCAAGA 2822 0.3515332555193337 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2808 0.34978929181370977 No Hit CTTTAGGACGTGAAATATGGCGAGG 2260 0.2815255696221453 No Hit GGTATCAACGCAGAGTACTTTTTTT 2086 0.25985059213796247 No Hit TATCAACGCAGAGTACTTTTTTTTT 2024 0.2521273242987709 No Hit GTCCTACAGTGTGCATTTCTCATTT 1621 0.20192608334402548 No Hit CTGAAGGACCTGGAATATGGCGAGA 1242 0.15471449445606394 No Hit CTGTAGGACCTGGAATATGGCGAGA 1150 0.14325416153339254 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1021 0.12718478167442937 No Hit GAATATGGCAAGAAAACTGAAAATC 1012 0.12606366214938544 No Hit ATTTAGAAATGTCCACTGTAGGACG 1008 0.12556538680492146 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1004 0.1250671114604575 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 948 0.11809125663796184 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 907 0.1129839343572061 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 495 0.0 14.501428 1 TAAGGCC 40 0.0054737357 14.17124 4 CGGTAGG 50 0.0010409674 13.957624 1 GTATACT 75 1.0278491E-6 13.856323 4 GTATAAG 70 6.9381604E-5 12.818227 1 TAGGACC 480 0.0 12.203011 4 ATGTCGA 70 1.1468113E-4 12.147533 17 GTGGTAT 115 3.6056008E-8 12.137064 1 GTCGAGG 55 0.0031946592 12.024831 19 AACAACC 55 0.0031961496 12.024081 7 CTCTAGG 135 2.2064341E-9 11.815979 1 TATGTCG 65 8.3930296E-4 11.628408 16 TGAACAG 75 2.180826E-4 11.336991 5 TAGGACT 135 5.136826E-9 11.197028 4 GGCGAGG 790 0.0 11.122426 19 TGTAGGA 1510 0.0 11.026128 2 GCGCCAC 60 0.0061186887 11.022075 13 CGCCACC 60 0.0061186887 11.022075 14 AGGACCT 880 0.0 10.843144 5 CTGTAGG 1545 0.0 10.840873 1 >>END_MODULE